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Elmo1 (NM_080288) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220091
Elmo1 (Myc-DDK-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C. elegans) (Elmo1), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $665.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Elmo1
Synonyms 6330578D22Rik; C230095H21Rik; CED-12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220091 representing NM_080288
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCCGCCTTCGGACATCGTGAAGGTGGCCATAGAATGGCCTGGCGCCTACCCTAAACTCATGGAGA
TTGACCAGAAAAAGCCACTGTCTGCCATTATAAAGGAAGTCTGTGATGGGTGGTCTCTTGCCAACCATGA
ATATTTTGCACTGCAGCACGCTGATAGTTCAAACTTCTACATCACAGAAAAGAATCGCAACGAAATAAAA
AATGGCACGATCCTTCGATTAACCACATCTCCAGCTCAGAATGCCCAGCAGCTCCATGAACGAATACAGT
CATCAAGTATGGATGCCAAGTTGGAAGCCCTGAAGGACTTGGCCAGCCTCTCCCGGGATGTTACCTTTGC
TCAGGAGTTCATAAACCTGGATGGCATCTCACTTCTCACTCAGATGGTGGAAAGTGGCACTGAACGATAC
CAGAAGTTGCAGAAGATTATGAAGCCCTGCTTTGGAGACATGCTGTCCTTCACTCTCACAGCCTTTGTGG
AGTTGATGGACCACGGCATTGTATCATGGGATACATTCTCAGTGGCCTTCATTAAGAAGATAGCCAGCTT
TGTGAACAAGTCTGCCATAGACATCTCAATCCTCCAGCGGTCCTTGGCCATTCTGGAGTCAATGGTTCTC
AACAGTCATGACCTCTACCAAAAGGTAGCCCAGGAGATCACCATTGGCCAACTCATTCCTCATCTTCAGG
GGACAGACCAAGAAATACAAACTTACACCATTGCTGTAATCAACGCACTTTTCCTCAAAGCTCCTGATGA
GAGGAGACAGGAGATGGCGAATATTTTGGCTCAGAAGCAGCTGCGCTACATCATTTTAACACATGTCATC
CGAGCACAGCGGGCCATCAACAATGAGATGGCACACCAGTTATATGTTCTCCAAGTGCTGACCTTTAACC
TTCTGGAAGACAGGATGATGACTAAGATGGACCCCCAGGACCAGGCCCAAAGGGACATCATATTTGAACT
TCGAAGAATTGCTTTCGATGCAGAGTCAGAACCTAATAACAGCAGTGGCAGCATGGAGAAACGCAAGTCC
ATGTACACTCGGGATTATAAAAAACTTGGCTTCATTAATCACGTCAATCCTGCCATGGACTTCACACAGA
CCCCTCCTGGGATGCTGGCTCTGGACAACATGCTGTATTTCGCTAAGCACCACCAGGATGCATACATCCG
GATCGTGCTAGAGAACAGCAGCCGAGAAGATAAACATGAGTGCCCCTTCGGCCGCAGCAGTATAGAGCTG
ACCAAGATGCTGTGTGAGATCCTCAAAGTGGGCGAGCTGCCTAGTGAGACCTGCAACGACTTTCACCCGA
TGTTCTTCACCCATGACAGATCTTTTGAGGAATTCTTCTGCATTTGCATTCAACTCCTGAACAAAACATG
GAAGGAAATGAGGGCAACATCTGAAGACTTCAACAAGGTAATGCAGGTGGTGAAGGAGCAGGTTATGAGA
GCGCTTACAACCAAGCCTAGTTCCCTGGACCAGTTCAAAAGCAAGCTGCAAAACCTGAGCTACACCGAGA
TCCTGAAAATCCGCCAGTCTGAGAGGATGAACCAGGAAGATTTCCAGTCTCGCCCGATTTTGGAACTAAA
GGAGAAGATCCAGCCAGAAATCTTAGAGCTGATTAAACAGCAGCGCCTGAACCGCCTTGTGGAAGGGACC
TGCTTTAGGAAACTCAATGCTCGCCGGAGACAAGACAAGTTTTGGTATTGTCGGCTTTCACCAAATCACA
AGGTCTTACATTATGGCGACTTGGAAGAGAGCCCCCAAGGAGAAGTGCCCCACGATTCCCTGCAGGACAA
ATTGCCGGTGGCAGATATCAAAGCAGTGGTGACGGGAAAGGACTGCCCTCATATGAAAGAGAAAGGTGCC
CTTAAACAAAACAAGGAGGTGCTTGAACTTGCTTTCTCCATCTTATACGACTCAAATTGCCAACTGAACT
TCATTGCTCCTGATAAGCATGAGTACTGCATCTGGACAGATGGGCTGAATGCACTGCTTGGGAAGGACAT
GATGAGTGACCTGACACGCAATGACCTGGACACCCTGCTGAGCATGGAGATCAAGCTTCGCCTGCTGGAC
CTGGAAAACATCCAGATCCCGGATGCACCTCCGCCTATCCCCAAGGAACCTAGCAACTATGACTTTGTCT
ATGACTGTAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220091 representing NM_080288
Red=Cloning site Green=Tags(s)

MPPPSDIVKVAIEWPGAYPKLMEIDQKKPLSAIIKEVCDGWSLANHEYFALQHADSSNFYITEKNRNEIK
NGTILRLTTSPAQNAQQLHERIQSSSMDAKLEALKDLASLSRDVTFAQEFINLDGISLLTQMVESGTERY
QKLQKIMKPCFGDMLSFTLTAFVELMDHGIVSWDTFSVAFIKKIASFVNKSAIDISILQRSLAILESMVL
NSHDLYQKVAQEITIGQLIPHLQGTDQEIQTYTIAVINALFLKAPDERRQEMANILAQKQLRYIILTHVI
RAQRAINNEMAHQLYVLQVLTFNLLEDRMMTKMDPQDQAQRDIIFELRRIAFDAESEPNNSSGSMEKRKS
MYTRDYKKLGFINHVNPAMDFTQTPPGMLALDNMLYFAKHHQDAYIRIVLENSSREDKHECPFGRSSIEL
TKMLCEILKVGELPSETCNDFHPMFFTHDRSFEEFFCICIQLLNKTWKEMRATSEDFNKVMQVVKEQVMR
ALTTKPSSLDQFKSKLQNLSYTEILKIRQSERMNQEDFQSRPILELKEKIQPEILELIKQQRLNRLVEGT
CFRKLNARRRQDKFWYCRLSPNHKVLHYGDLEESPQGEVPHDSLQDKLPVADIKAVVTGKDCPHMKEKGA
LKQNKEVLELAFSILYDSNCQLNFIAPDKHEYCIWTDGLNALLGKDMMSDLTRNDLDTLLSMEIKLRLLD
LENIQIPDAPPPIPKEPSNYDFVYDCN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080288
ORF Size 2181 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080288.2, NP_525027.1
RefSeq Size 5576 bp
RefSeq ORF 2184 bp
Locus ID 140580
UniProt ID Q8BPU7
Cytogenetics 13 A2
MW 83.9 kDa
Summary Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_080288" in other vectors (4)
SKU Description Size Price
MC221117 Elmo1 (untagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C. elegans) (Elmo1), transcript variant 1, (10ug) 10 ug
$733.00
MG220091 Elmo1 (tGFP-tagged) - Mouse engulfment and cell motility 1 ced-12 homolog (C. elegans) (Elmo1) transcript variant 1, (10ug) 10 ug
$866.00
MR220091L3 Lenti ORF clone of Elmo1 (Myc-DDK-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C. elegans) (Elmo1), transcript variant 1 10 ug
$966.00
MR220091L4 Lenti ORF clone of Elmo1 (mGFP-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C. elegans) (Elmo1), transcript variant 1 10 ug
$966.00

Plasmid Map for MR220091

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220091
Circular map for MR220091

file-alt Citations

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