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Cacfd1 (NM_029862) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220034
Cacfd1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 5930434B04 gene (5930434B04Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Cacfd1 or NM_029862"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cacfd1
Synonyms 5930434B04Rik; mFwe
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220034 representing NM_029862
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGGCTCGGGCGCCGCCGGAGCGGCCGCGGGCCCAGCCCCGCCGGCACAGGAAGAGGGCATGACTT
GGTGGTACCGCTGGCTGTGCCGCCTGGCGGGAGTGCTAGGAGCGGTGTCCTGTGCCATCTCTGGACTCTT
CAACTGCGTCACTATCCACCCTCTGAACATCGCAGCTGGCGTGTGGATGATCATGAACGCCTTCATCCTG
TTGTTGTGCGAGGCTCCCTTCTGCTGCCAGTTTGTGGAGTTTGCAAACACAGTAGCTGAGAAGGTTGACC
GGCTGCGCTCCTGGCAGAAGGCTGTCTTCTACTGCGGGATGGCCATCGTCCCCATCGTCATGAGCCTCAC
CCTGACTACACTGCTGGGCAATGCCATCGCCTTTGCCACGGGGGTGCTGTATGGACTGTCTGCCCTGGGC
AAAAAGGGCGATGCCATTTCTTATGCTCGGATCCAGCAGCAGAGACAGCAGGCAGATGAGGAGAAGCTGG
CGGAGACTTTCGAGGGGGAACTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220034 representing NM_029862
Red=Cloning site Green=Tags(s)

MSGSGAAGAAAGPAPPAQEEGMTWWYRWLCRLAGVLGAVSCAISGLFNCVTIHPLNIAAGVWMIMNAFIL
LLCEAPFCCQFVEFANTVAEKVDRLRSWQKAVFYCGMAIVPIVMSLTLTTLLGNAIAFATGVLYGLSALG
KKGDAISYARIQQQRQQADEEKLAETFEGEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029862
ORF Size 513 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029862.4, NP_084138.1
RefSeq Size 1608 bp
RefSeq ORF 516 bp
Locus ID 381356
UniProt ID Q8BG21
Cytogenetics 2 A3
MW 18.8 kDa
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Documents
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"NM_029862" in other vectors (4)
SKU Description Size Price
MC214706 Cacfd1 (untagged) - Mouse RIKEN cDNA 5930434B04 gene (5930434B04Rik), (10ug) 10 ug
$330.00
MG220034 Cacfd1 (tGFP-tagged) - Mouse RIKEN cDNA 5930434B04 gene (5930434B04Rik), (10ug) 10 ug
$500.00
MR220034L3 Lenti ORF clone of Cacfd1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 5930434B04 gene (5930434B04Rik) 10 ug
$600.00
MR220034L4 Lenti ORF clone of Cacfd1 (mGFP-tagged) - Mouse RIKEN cDNA 5930434B04 gene (5930434B04Rik) 10 ug
$600.00

Plasmid Map for MR220034

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220034
Circular map for MR220034

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