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Ttll8 (NM_172818) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219988
Ttll8 (Myc-DDK-tagged) - Mouse tubulin tyrosine ligase-like family, member 8 (Ttll8)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $762.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ttll8
Synonyms 1700019P01Rik; 4933401B17
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219988 representing NM_172818
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGTGTCCCCCGACCCCAAACCCTCCTTTCAGGCCTCCCTCCCACACCCGGGTCCTCCGAACCCCAC
CCTTGCCTCCCTGGGTATGTCTGAACTCTAAATCTTTGTCAACAGGGGTTGGGGGCCAAAAGAACCAGCT
ACGAGAGGCGAGCATGGAGAACGGCGAGAGGAAGAAGCTGTCTTCCACTTTGTCAGATGGAGATCACAAA
GAAGAAAATAAGTTAAAACAAGGAATTCCCCAAGATTTGTCTTCTTCCCCCAAATTAGACAGATACAAAA
TAGCAAGACAGTTAACAGAAAAAGCCATCAAGGAGAGGAAGATTTTCTCCATCTATGGACACTACCCTGT
CATCCGGGCCACCTTGCGAAGAAAGGGCTGGGTGGAGAAGAAGTTCAATTTCTTCCCCAAAGCTCTCCAG
AACCTTGGAAGTGAAGATAAAAGTGCTGAGACCAAAGAAAATCAAGAAATAGCCTTGGAGAGATTCGATG
ATATCCACGATGTGATGTCCAGGTTGGTAAAGAACGAGATCCCATACCTCCTCTGGACTATCAAGAGGGA
TGTTGTGGACTACCACAGCCTGACCTGTGACCAGATGCTAAATCACTACGGGAAGACAGCATCATTCACA
ACCAAGATTGGACTCTGCCTGAACATGAGAAGTCTGCCTTGGTACGTCCAAGCCAACCCGAACACCTTCT
TCCCACGGTGCTATGGCCTTTGCACTGAGAGCGAGAAGCAGGAATTCTTGGATGACTTCCGGCGCACGGT
GGCTGCCAGCATCCTCAAGTGGGTAGTCCTTCACCAGAATTACTGCAGCAAAGTCAAGGGCAAGAGTAAG
AAGGAGGAGGCCAAGAACAGTGACCCCAGCCCCAAGAAAGATCCTGAAAATCCTGACCTCAAGCTACCAA
GCCTCTCGGGACAGGTTGTAGACACAGCTTGCAAGGTGTGCCAGGCCTACCTGGGACAGCTAGAGCATGA
AGACATAGACGTATCGGAGGCCAGCACCGAGGCCTTGTCTGAGGAGGAATGGAATGACCTGACACAGCAG
TACTATCTACTGGTTCATGGCAATGCTTCCATCACTGATTCGAAAAGTTACTTTGCGCAATGCCAAGCTC
TGCTGAGTAAGATCAGTTCTGTGAACCCTCAGACAGAGATCGATGGGATCCGGAACATCTGGATCATAAA
GCCTGCAGCCAAGTCCCGGGGTCGAGATATTGTGTGTATGGACCGTGTGGAGAACATCCTGAGTCTGGTG
GCCGCAGACAGCCAGACCACAAAGGACAACAAATGGGTGGTCCAGAAGTACATCGAGACCCCAATGCTCA
TCTACGACACCAAGTTTGACATCAGACAGTGGTTCCTCGTCACAGACTGGAATCCCCTAACCATCTGGTT
CTACAAAGAAAGCTACCTCCGCTTCTCCACACAGCGCTTCTCCCTGGACAAGCTGGACAGCGCCATCCAC
CTGTGTAACAACTCCATCCAGAGGCGCCTCAAAAATGATAAAGAGCGCAGTCCGCTGCTACCTTGTCATA
ACATGTGGACCAGCACTCGTTTCCAGGAGTACCTGCAGAAGAGGGGCCGAGGAGGAACGTGGGGTAGCAT
CATCTACCCGTCTATGAAAAGAGCTGTCACCAATGCCATGAGGGTAGCCCAGGACCACGTAGAAGCCCGT
AAGAACAGCTTCGAGCTCTACGGAGCCGACTTCATCCTGGGGCGAGACTTCAAGCCATGGCTTATCGAGA
TCAACTCCAGCCCCACCATGCACCCCTCCACTCCCGTCACAGCTCAGCTCTGTGCCCAGGTGCAGGAGGA
CACCATCAAGGTGGTGGTGGATCGCAAACTGGACCGAAACTGTGACATCGGCAACTTTGAGCTTCTGTGG
CGGCAGCCTGCCGTGGAGCTGCCACCGTTCAACGGGTCTGACCTCTGTGTGGAAGGTATCAGCGTGAAGA
AAGCCAAGAAGCAAATGCCGCCCATTGCCAGCGTCGGCCTCTCAGAATCACTCTTAGATGCTCCACCCAA
AGTGCGGAGTGCCCGGGCCCTGATGGAAACAGTGATCAGGCCTCCAAGAACGACCGTGCGGCAGGACTGG
AAACGGGAAGAAGCAAAGGTACTTTCAACCACTTGGTCCATGCCAGTAATGGATGCAGAGGTCAGAGGTC
GAGCAAAGCCCATCTATGCATTCGAGGTCAATGACTACCAACATGTAGACAATAAGTCCCACAAGAGCGG
TTATACCAGAGTCCAATCCAGTAAAGTCCCAGGGGTAACCTTAACAAGTGCACAGCACCCTGCCCTGTTT
GCACAGACTATGAAACCCACACAGATGACCTCGTCTCCACCACCCACTGCGTCAGGTAACCATAGAGACT
CTTCGCCTTTCTGTCCCATTGTATTTGAGGAACTCTGGCTCCACCCCAATTCTCAGAGACGACCTTCAAG
CTGTATTCTCCAAAGCAGGGCACAGGGCTGGATCAGGGGGATCCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219988 representing NM_172818
Red=Cloning site Green=Tags(s)

MSCPPTPNPPFRPPSHTRVLRTPPLPPWVCLNSKSLSTGVGGQKNQLREASMENGERKKLSSTLSDGDHK
EENKLKQGIPQDLSSSPKLDRYKIARQLTEKAIKERKIFSIYGHYPVIRATLRRKGWVEKKFNFFPKALQ
NLGSEDKSAETKENQEIALERFDDIHDVMSRLVKNEIPYLLWTIKRDVVDYHSLTCDQMLNHYGKTASFT
TKIGLCLNMRSLPWYVQANPNTFFPRCYGLCTESEKQEFLDDFRRTVAASILKWVVLHQNYCSKVKGKSK
KEEAKNSDPSPKKDPENPDLKLPSLSGQVVDTACKVCQAYLGQLEHEDIDVSEASTEALSEEEWNDLTQQ
YYLLVHGNASITDSKSYFAQCQALLSKISSVNPQTEIDGIRNIWIIKPAAKSRGRDIVCMDRVENILSLV
AADSQTTKDNKWVVQKYIETPMLIYDTKFDIRQWFLVTDWNPLTIWFYKESYLRFSTQRFSLDKLDSAIH
LCNNSIQRRLKNDKERSPLLPCHNMWTSTRFQEYLQKRGRGGTWGSIIYPSMKRAVTNAMRVAQDHVEAR
KNSFELYGADFILGRDFKPWLIEINSSPTMHPSTPVTAQLCAQVQEDTIKVVVDRKLDRNCDIGNFELLW
RQPAVELPPFNGSDLCVEGISVKKAKKQMPPIASVGLSESLLDAPPKVRSARALMETVIRPPRTTVRQDW
KREEAKVLSTTWSMPVMDAEVRGRAKPIYAFEVNDYQHVDNKSHKSGYTRVQSSKVPGVTLTSAQHPALF
AQTMKPTQMTSSPPPTASGNHRDSSPFCPIVFEELWLHPNSQRRPSSCILQSRAQGWIRGIP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172818
ORF Size 2496 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172818.3, NP_766406.2
RefSeq Size 2976 bp
RefSeq ORF 2499 bp
Locus ID 239591
UniProt ID A4Q9F1
Cytogenetics 15 E3
MW 94.9 kDa
Summary Monoglycylase which modifies both tubulin and non-tubulin proteins, generating side chains of glycine on the gamma-carboxyl groups of specific glutamate residues of target proteins. Monoglycylates tubulin, with a preference for alpha-tubulin toward beta-tubulin. Has the ability to modify non-tubulin proteins such as ANP32A, ANP32B, SET and NCL. Involved in the side-chain initiation step of the glycylation reaction by adding a single glycine chain to generate monoglycine side chains. Not involved in elongation step of the polyglycylation reaction.UniProtKB/Swiss-Prot Function
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"NM_172818" in other vectors (4)
SKU Description Size Price
MC221968 Ttll8 (untagged) - Mouse tubulin tyrosine ligase-like family, member 8 (Ttll8), (10ug) 10 ug
$839.00
MG219988 Ttll8 (tGFP-tagged) - Mouse tubulin tyrosine ligase-like family member 8 (Ttll8), (10ug) 10 ug
$962.00
MR219988L3 Lenti ORF clone of Ttll8 (Myc-DDK-tagged) - Mouse tubulin tyrosine ligase-like family, member 8 (Ttll8) 10 ug
$1,062.00
MR219988L4 Lenti ORF clone of Ttll8 (mGFP-tagged) - Mouse tubulin tyrosine ligase-like family, member 8 (Ttll8) 10 ug
$1,062.00

Plasmid Map for MR219988

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219988
Circular map for MR219988

file-alt Citations

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