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Kctd19 (NM_177791) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219964
Kctd19 (Myc-DDK-tagged) - Mouse potassium channel tetramerisation domain containing 19 (Kctd19)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $871.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kctd19
Synonyms 4922504H04Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219964 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGCCTGGCGGGCTCCATGAGTCAGCAGAGGATTTGTTTCACTTCAATGTCGGGGGCTGGCATT
TCTCAGTTCCCAGGAGCAAACTTGCTCAGTTCCCAGACTCGCTGTTGTGGAAAGAGGCTTCAGCCCTGAC
CTCTTCAGAGAACCAGCGGCTCTTCATTGACAGAGACGGCTCCACGTTTAGGCATGTGCACTATTACCTC
TACACGTCCAAGCTCTCCTTCTCCAGTTGTGCAGAACTGAACTTGCTCTATGAGCAAGCCCTGGGTTTGC
AGCTGATGCCCTTGCTTCAGACCCTAGATAACCTGAAGGAAGGGAGACACCACCTCCGTGTGCGGCCTGC
CGACATTCCTGTGGCTGAGAGAGCATCCCTGAACTACTGGCGGACATGGAAGTGTATCAGCAAGCCCTCG
GATTTTCCAATAAAAAGCCCCGCCTTCACAGGTCTTCATGACAAGGCCCCTTTGGGACTCATGGACACAC
CGCTGTTGGACACAGAAGAGGAAGTGCACTACTGCTTCTTACCCCTCGACTTGGTGGCCAAGTACCCAAG
CCTAGTGACCGAAGACAATCTGCTGTGGCTGGCTGAAACGGTGGCCCTGATCGAGTGCGAATGCAGCGAG
TTCCGCTTCATTGTGAATTTTCTACGCTCACACAAGATTTTACTACCAGATAACTTCTCCAACATAGATG
TGTTAGAAGCAGAAGTAGAAATTCTGGAAATCCCTGAGCTTACAGAAGCTGTACGGTTGTACCGGATGAA
TATGGCGTTGCAACCAGCGTTGCCAGGGCCGAAGGGAAGCCAGCTTAGCCTGGAGCAGGAGGAGCCTAGC
CTGGGTGGCTGTTCCCGGACCTCCTGCCCACCTCTAAGTCCTGGAAAGGGGGGTCGTACAGCCAGTGTGG
AGTCTGTGAAACCACTGTACTTGATGGCCCTGGGCCTGCTGGTCAAGTATCCAGACTCTGCACTGGGACA
GCTGCGCATCGAGAGCACGCTGGACGGAAGCAGACTATACATCACAGGGAACGGGGCCCTTTTCCAGCAC
GTCAGGAACTGGCTGGGCACTTGTCGGCTGCCCCTGACTGAGACCATCTCCGAGGTGTATGAGCTCTGCG
CCTTCCTGGACAAGAGGGACATCACCTACGAGCCAATGAAAGTGGCTCTGAAGACTCATCTGGAGCCAAG
GACTTTGCTGCCCGTGGATGTGCTCAGTGAGGAGTGGACAGCTGAGGTCACGATATACTCACCACAGCAG
ATCATCAAGCTTTATGTTGGAAGCCATTGGTATGCAACGACCCTGCAGACCCTGATGAAGTACCCAGAAC
TGCTATCCAACACTCAGAGAGTGTACTGGATCGCCTACGGACAGACCTTGCTCATTCACGGGGACGGCCA
AATGTTCCGACACATTCTCAACTTCCTGAGACTTGGAAAACTGTTTTTACCATCAGAATTTAAGGAATGG
CCTCTCTTCTGCCAGGAGGTGGAGGAGTATCATATTCCAGCCCTCTCAGAAGCCCTGGCACAATGCGAAG
CATACAAGTCATGGACTCAGGAGAAAGAATCTGAAAATGAAGAAGCTTTTCCCATTAGGAAGCTGCATGT
GGTGACCGAAGGGACAGGGCCAATGGCAGAGTTCAGCAGAGATGCCAAGGAAACCACAGCCTGCATGCCT
GTGGACTTCCAGGAATGCAGTGACAGGACTCCATGGAACAAGGCCAAAGGGAACCTGACCAGGTCCAGCC
AGATGGAAGAGGCTGAGCAGTATACCCGGACTATTCAGGTCTCCCTCTGCAGAAATGCCAAGAGGGGAGG
CAATCCCAGCACATACTCACACTGCTCTGGTTTATGTGCCAACCCCAGACATTGGGGAGGTCACTCTGAG
AGTCCCCCCAAGAAGAAGTGCACCACTATAAACCTCACACAGAAACCTGATGCCAAAGACCCTCCTGTCA
CCCCCATGCAAAAACTCATATCTTTAGTCAGGGAATGGGACATGGTCAACTGTAAACAGTGGGAATTCCA
GCCACTGCCAGCCTCCAGGAGCAGCTCTGTGGAGGAGGCTAGCCTGCATGTCCCCTCGGGAAGTGAGGCT
GCTCCGCAGCCTGGTACCTCAGCTGCTTGGAAAGCTCATTCCATAACCTCTGAGAAGGATGCTGGTCCCC
AGACTGGACCCGGAGCTGGAGTGAAAGACAAGGGTCCAGAGCCAACCTTTAAGCCATACTTCCCTACAAA
AAGAGCTATTACCCTAAAGGACTGGGGCAAGCAGAGGCCCAAGGACAGAGAAAGCCCTGCTCCTGAGCAG
CCTCTGCCTAATGCTAACGGGACCGACAATCCAGGGGCCATCCTCAAAGTGGCTCACCCCCCTGTGGTGG
GTAATGATGGCTCCTGCATGTTCTTTGAAGACAGCATTATCTACACCACCCAAATGGATAACATCAAGCA
TACATCACTCACAGCCAGCCCCCAACTTCGAGAAGTGACTTTTCTGAGCTTCTCTCTGTCCTGGGAGGAG
ATGTTTTATGCACAGAAATGTCACCGCTTCCTGACCGACATCATCTTGGATTCCATCAGGCAAAAGGATC
CCAAAGCTATCACAGCCAAGGTGGTGTCTCTGGCCTACCGACTGTGGACACTGAACATCAGCCCCAAGCA
GTTCGTGGTGGATCTGCTGGCCATCGCTGGCTTCAAGGATGACCGGCACACTCAGGAGCGTCTGTACAGT
TGGGTAGAGCTCACACTGCCTTTCGCCAGAAAATATGGCCGTTGCGTGGACCTGCTCATCCAGAGGGGAC
TGTCCAGGTCTGTCTCTTATTCAGTCCTGGGCAAGTATTTACACGAGGGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219964 protein sequence
Red=Cloning site Green=Tags(s)

MEEPGGLHESAEDLFHFNVGGWHFSVPRSKLAQFPDSLLWKEASALTSSENQRLFIDRDGSTFRHVHYYL
YTSKLSFSSCAELNLLYEQALGLQLMPLLQTLDNLKEGRHHLRVRPADIPVAERASLNYWRTWKCISKPS
DFPIKSPAFTGLHDKAPLGLMDTPLLDTEEEVHYCFLPLDLVAKYPSLVTEDNLLWLAETVALIECECSE
FRFIVNFLRSHKILLPDNFSNIDVLEAEVEILEIPELTEAVRLYRMNMALQPALPGPKGSQLSLEQEEPS
LGGCSRTSCPPLSPGKGGRTASVESVKPLYLMALGLLVKYPDSALGQLRIESTLDGSRLYITGNGALFQH
VRNWLGTCRLPLTETISEVYELCAFLDKRDITYEPMKVALKTHLEPRTLLPVDVLSEEWTAEVTIYSPQQ
IIKLYVGSHWYATTLQTLMKYPELLSNTQRVYWIAYGQTLLIHGDGQMFRHILNFLRLGKLFLPSEFKEW
PLFCQEVEEYHIPALSEALAQCEAYKSWTQEKESENEEAFPIRKLHVVTEGTGPMAEFSRDAKETTACMP
VDFQECSDRTPWNKAKGNLTRSSQMEEAEQYTRTIQVSLCRNAKRGGNPSTYSHCSGLCANPRHWGGHSE
SPPKKKCTTINLTQKPDAKDPPVTPMQKLISLVREWDMVNCKQWEFQPLPASRSSSVEEASLHVPSGSEA
APQPGTSAAWKAHSITSEKDAGPQTGPGAGVKDKGPEPTFKPYFPTKRAITLKDWGKQRPKDRESPAPEQ
PLPNANGTDNPGAILKVAHPPVVGNDGSCMFFEDSIIYTTQMDNIKHTSLTASPQLREVTFLSFSLSWEE
MFYAQKCHRFLTDIILDSIRQKDPKAITAKVVSLAYRLWTLNISPKQFVVDLLAIAGFKDDRHTQERLYS
WVELTLPFARKYGRCVDLLIQRGLSRSVSYSVLGKYLHEG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177791
ORF Size 2850 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177791.3, NP_808459.1
RefSeq Size 3076 bp
RefSeq ORF 2853 bp
Locus ID 279499
UniProt ID Q562E2
Cytogenetics 8 D3
MW 107.2 kDa
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Documents
Resources
"NM_177791" in other vectors (4)
SKU Description Size Price
MC222818 Kctd19 (untagged) - Mouse potassium channel tetramerisation domain containing 19 (Kctd19), (10ug) 10 ug
$958.00
MG219964 Kctd19 (tGFP-tagged) - Mouse potassium channel tetramerisation domain containing 19 (Kctd19), (10ug) 10 ug
$1,071.00
MR219964L3 Lenti ORF clone of Kctd19 (Myc-DDK-tagged) - Mouse potassium channel tetramerisation domain containing 19 (Kctd19) 10 ug
$1,171.00
MR219964L4 Lenti ORF clone of Kctd19 (mGFP-tagged) - Mouse potassium channel tetramerisation domain containing 19 (Kctd19) 10 ug
$1,171.00

Plasmid Map for MR219964

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219964
Circular map for MR219964

file-alt Citations

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