Ascc3 (NM_198007) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR219721
Ascc3 (Myc-DDK-tagged) - Mouse activating signal cointegrator 1 complex subunit 3 (Ascc3), transcript variant 2
  $1,995.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ascc3
Synonyms ASC1p200; B630009I04Rik; BC023451; D430001L07Rik; D630041L21; Helic1; RNAH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198007
ORF Size 6594 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198007.2, NP_932124.2
RefSeq Size 7477 bp
RefSeq ORF 6597 bp
Locus ID 77987
UniProt ID E9PZJ8
Cytogenetics 10 B3
MW 250.6 kDa
Summary 3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3, enabling ALKBH3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions. Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation.UniProtKB/Swiss-Prot Function
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"NM_198007" in other vectors (2)
SKU Description Size Price
MC225222 Ascc3 (untagged) - Mouse activating signal cointegrator 1 complex subunit 3 (Ascc3), transcript variant 2, (10ug) 10 ug
$1,996.00
MG219721 Ascc3 (tGFP-tagged) - Mouse activating signal cointegrator 1 complex subunit 3 (Ascc3) transcript variant 2, (10ug) 10 ug
$2,195.00

Plasmid Map for MR219721

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Cloning scheme for MR219721

Circular map for MR219721

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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