Npy4r (NM_008919) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR219705
Ppyr1 (Myc-DDK-tagged) - Mouse pancreatic polypeptide receptor 1 (Ppyr1)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Npy4r
Synonyms NYYR-D; Ppyr1; Y4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219705 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATACCTCTCATTTCTTGGCCCCTCTCTTCCCAGGATCCCTACAGGGTAAGAATGGGACCAATCCAT
TGGATTCCCCCTATAATTTCTCTGATGGCTGCCAGGATTCGGCAGAGCTGTTGGCCTTCATCATCACCAC
CTACAGCATTGAGACCATCTTGGGGGTCCTGGGAAACCTCTGCTTGATATTTGTGACCACAAGACAAAAG
GAAAAGTCCAATGTGACCAACCTACTCATTGCCAACCTGGCCTTCTCTGACTTCCTCATGTGCCTCATCT
GCCAACCACTCACAGTCACCTACACCATCATGGATTACTGGATCTTTGGTGAAGTCCTTTGCAAGATGTT
AACTTTCATCCAGTGTATGTCAGTGACAGTCTCCATCCTCTCACTGGTCCTTGTGGCCCTGGAGAGGCAC
CAGCTCATTATCAATCCAACAGGCTGGAAACCCAGTATTTTCCAGGCCTACCTGGGGATTGTGGTCATCT
GGTTCGTCTCTTGTTTCCTTTCCTTGCCGTTCCTGGCCAACAGCACCCTGAATGACCTCTTCCACTACAA
CCACTCTAAGGTTGTAGAGTTTCTGGAAGACAAGGTCGTCTGCTTTGTGTCCTGGTCTTCAGATCACCAC
CGTCTCATCTATACCACTTTTCTGCTGCTCTTTCAGTACTGCATCCCTCTAGCCTTCATCCTGGTCTGCT
ACATACGCATCTACCAGCGCCTGCAGAGGCAGAAGCATGTGTTCCATGCGCACGCTTGCAGCTCACGAGC
GGGACAGATGAAGCGGATCAACAGCATGCTCATGACAATGGTGACTGCCTTTGCAGTTCTCTGGCTACCC
CTGCATGTGTTCAACACTCTGGAGGACTGGTACCAGGAAGCCATCCCTGCTTGCCATGGCAACCTCATCT
TCTTGATGTGCCACCTGTTGGCCATGGCTTCCACCTGTGTCAACCCTTTCATCTATGGCTTTCTCAACAT
CAACTTCAAGAAGGATATCAAGGCTCTGGTGCTGACCTGCCATTGCAGGTCACCTCGAGGGGAGTCTGAG
CATCTGCCCCTGTCCACTGTTCACACGGACCTATCCAAGGGATCGATGAGGATGGGTAGCAAGTCTAACT
TCATA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR219705 protein sequence
Red=Cloning site Green=Tags(s)

MNTSHFLAPLFPGSLQGKNGTNPLDSPYNFSDGCQDSAELLAFIITTYSIETILGVLGNLCLIFVTTRQK
EKSNVTNLLIANLAFSDFLMCLICQPLTVTYTIMDYWIFGEVLCKMLTFIQCMSVTVSILSLVLVALERH
QLIINPTGWKPSIFQAYLGIVVIWFVSCFLSLPFLANSTLNDLFHYNHSKVVEFLEDKVVCFVSWSSDHH
RLIYTTFLLLFQYCIPLAFILVCYIRIYQRLQRQKHVFHAHACSSRAGQMKRINSMLMTMVTAFAVLWLP
LHVFNTLEDWYQEAIPACHGNLIFLMCHLLAMASTCVNPFIYGFLNINFKKDIKALVLTCHCRSPRGESE
HLPLSTVHTDLSKGSMRMGSKSNFI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008919
ORF Size 1125 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008919.4, NP_032945.3
RefSeq Size 1903 bp
RefSeq ORF 1128 bp
Locus ID 19065
UniProt ID Q61041
Cytogenetics 14 20.8 cM
MW 42.6 kDa
Summary Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PP >> PYY >= NPY.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008919" in other vectors (4)
SKU Description Size Price
MC209222 Npy4r (untagged) - Mouse pancreatic polypeptide receptor 1 (Ppyr1), (10ug) 10 ug
$457.00
MG219705 Ppyr1 (tGFP-tagged) - Mouse pancreatic polypeptide receptor 1 (Ppyr1), (10ug) 10 ug
$657.00
MR219705L3 Lenti ORF clone of Ppyr1 (Myc-DDK-tagged) - Mouse pancreatic polypeptide receptor 1 (Ppyr1) 10 ug
$757.00
MR219705L4 Lenti ORF clone of Ppyr1 (mGFP-tagged) - Mouse pancreatic polypeptide receptor 1 (Ppyr1) 10 ug
$757.00

Plasmid Map for MR219705

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Cloning scheme for MR219705

Circular map for MR219705

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