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Rab7b (NM_145509) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219682
5430435G22Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5430435G22 gene (5430435G22Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rab7b
Synonyms 5430435G22Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219682 representing NM_145509
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCCCCGAAAGAAAGTGGACTTGAAACTTATCATTGTTGGTGCACTTGGCGTGGGAAAGACCTCTC
TTCTTCACCAATATGTACACAAAACATTTTTCGAGGAATACCAGACCACACTGGGGGCCAGCATCCTCTC
CAAAATCATCATATTGGATGACACAACTTTGAAGCTGCAGATCTGGGACACAGGTGGTCAGGAGCGGTTC
CGCTCAATGGTATCAACATTCTACAAAGGTTCCGATGGCTGTATCCTGGCATTTGATGTTACTGATCCAG
AGTCCTTTGAAGCCCTGGATATCTGGAGGGATGATGTCCTGGCAAAGATTATCCCTATGGAGCAGTCATA
TCCCATGGTGGTGCTGGGGAACAAAATCGATCTGGAAGATAGGAAGGTGTCCCAGGAGGTGGTCCATGGG
TGGTGTAAAGAGAAGGATATGCCATATTTTGAAGTTAGTGCGAAGAATGACATCAATGTGGTACAAGCCT
TTGAGGTTCTGGCCAGCCGGGCTCTGTTGAGGTACCAGGGCACCGCAGAGAACCACCTCATAGACTCCAT
CAAGCTCTCTCCAGGCCAGCCAAAGAGCAGATGCTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219682 representing NM_145509
Red=Cloning site Green=Tags(s)

MNPRKKVDLKLIIVGALGVGKTSLLHQYVHKTFFEEYQTTLGASILSKIIILDDTTLKLQIWDTGGQERF
RSMVSTFYKGSDGCILAFDVTDPESFEALDIWRDDVLAKIIPMEQSYPMVVLGNKIDLEDRKVSQEVVHG
WCKEKDMPYFEVSAKNDINVVQAFEVLASRALLRYQGTAENHLIDSIKLSPGQPKSRCC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145509
ORF Size 597 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145509.3, NP_663484.1
RefSeq Size 4498 bp
RefSeq ORF 600 bp
Locus ID 226421
UniProt ID Q8VEA8
Cytogenetics 1 E4
MW 22.5 kDa
Summary Controls vesicular trafficking from endosomes to the trans-Golgi network (TGN). Acts as a negative regulator of TLR9 signaling and can suppress TLR9-triggered TNFA, IL6, and IFNB production in macrophages by promoting TLR9 lysosomal degradation. Also negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Promotes megakaryocytic differentiation by increasing NF-kappa-B-dependent IL6 production and subsequently enhancing the association of STAT3 with GATA1. Not involved in the regulation of the EGF- and EGFR degradation pathway.UniProtKB/Swiss-Prot Function
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Documents
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"NM_145509" in other vectors (4)
SKU Description Size Price
MC201452 5430435G22Rik (untagged) - Mouse RIKEN cDNA 5430435G22 gene (5430435G22Rik), (10ug) 10 ug
$300.00
MG219682 5430435G22Rik (tGFP-tagged) - Mouse RIKEN cDNA 5430435G22 gene (5430435G22Rik), (10ug) 10 ug
$500.00
MR219682L3 Lenti ORF clone of 5430435G22Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5430435G22 gene (5430435G22Rik) 10 ug
$600.00
MR219682L4 Lenti ORF clone of 5430435G22Rik (mGFP-tagged) - Mouse RIKEN cDNA 5430435G22 gene (5430435G22Rik) 10 ug
$600.00

Plasmid Map for MR219682

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219682
Circular map for MR219682

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