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Defa34 (NM_001177528) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219627
Gm15315 (Myc-DDK-tagged) - Mouse predicted gene 15315 (Gm15315)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Defa34
Synonyms ENSMUSG00000063206; Gm15315; OTTMUSG00000019924
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219627 representing NM_001177528
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGACAATTGTCCTCCTCTCTGCCCTTGTCCTGCTGGCCTTCCAAGTCCAGGCTGATCCTATCCAAA
AAACAGATGAAGAGACTAATACTGAGGAGCAGCCAGGGGAAGAGGACCAGGCTGTGTCTATCTCCTTTGG
AGGCCAAGAAGGGTCTGCTCTTCATGAAGAATTGTCAAAAAAGCTGATATGCTATTGTAGAATAAGAGGC
TGCAAAAGAAGAGAACGCGTTTTTGGGACCTGCAGAAATCTTTTTTTAACTTTCGTATTCTGCTGCAGC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219627 representing NM_001177528
Red=Cloning site Green=Tags(s)

MKTIVLLSALVLLAFQVQADPIQKTDEETNTEEQPGEEDQAVSISFGGQEGSALHEELSKKLICYCRIRG
CKRRERVFGTCRNLFLTFVFCCS

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001177528
ORF Size 279 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001177528.1, NP_001170999.1
RefSeq Size 376 bp
RefSeq ORF 282 bp
Locus ID 100041952
Cytogenetics 8
MW 10.5 kDa
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"NM_001177528" in other vectors (4)
SKU Description Size Price
MC215570 Gm15315 (untagged) - Mouse predicted gene 15315 (Gm15315), (10ug) 10 ug
$165.00
MG219627 Gm15315 (tGFP-tagged) - Mouse predicted gene 15315 (Gm15315), (10ug) 10 ug
$350.00
MR219627L3 Lenti ORF clone of Gm15315 (Myc-DDK-tagged) - Mouse predicted gene 15315 (Gm15315) 10 ug
$450.00
MR219627L4 Lenti ORF clone of Gm15315 (mGFP-tagged) - Mouse predicted gene 15315 (Gm15315) 10 ug
$450.00

Plasmid Map for MR219627

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219627
Circular map for MR219627

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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