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Cryge (NM_007777) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219597
Cryge (Myc-DDK-tagged) - Mouse crystallin, gamma E (Cryge)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Cryge or NM_007777"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cryge
Synonyms Cryg-2; Cryg-6; DGcry-2; Elo
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219597 representing NM_007777
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAAGATCACCTTCTATGAGGACCGCGGCTTCCAGGGCCGCCACTATGAGTGCAGCACCGACCACT
CCAACCTGCAGCCCTACTTCAGCCGCTGCAACTCTGTGCGCGTGGACAGTGGCTGCTGGATGCTCTATGA
GCAGCCCAACTTCGCAGGCTGCCAGTACTTCCTGCGTCGCGGGGACTACCCTGACTACCAGCAGTGGATG
GGTTTCAGTGACTCTGTCCGCTCCTGCCGCCTCATCCCCCACTCCAGTTCTCACAGGATCAAGATCTACG
AGCGAGAGGACTACAGAGGCCAAATGGTGGAGATCACAGACGACTGCTCCCACCTGCAGGACCGCTTCCA
CTTCAGTGACTTCCACTCCTTCCACGTGATGGAGGGCTACTGGGTCCTCTACGAGATGCCCAACTACCGG
GGGCGGCAGTACCTGCTCAGGCCTGGGGAGTACAGGCGCTACCACGACTGGGGCGCCATGAATGCCAGGG
TGGGCTCTCTGAGGAGAATCATGGATTTCTAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219597 representing NM_007777
Red=Cloning site Green=Tags(s)

MGKITFYEDRGFQGRHYECSTDHSNLQPYFSRCNSVRVDSGCWMLYEQPNFAGCQYFLRRGDYPDYQQWM
GFSDSVRSCRLIPHSSSHRIKIYEREDYRGQMVEITDDCSHLQDRFHFSDFHSFHVMEGYWVLYEMPNYR
GRQYLLRPGEYRRYHDWGAMNARVGSLRRIMDFY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007777
ORF Size 522 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007777.3, NP_031803.3
RefSeq Size 662 bp
RefSeq ORF 525 bp
Locus ID 12968
UniProt ID Q03740
Cytogenetics 1 32.8 cM
MW 21.2 kDa
Summary Crystallins are the dominant structural components of the vertebrate eye lens.UniProtKB/Swiss-Prot Function
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Documents
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"NM_007777" in other vectors (4)
SKU Description Size Price
MC208340 Cryge (untagged) - Mouse crystallin, gamma E (Cryge), (10ug) 10 ug
$330.00
MG219597 Cryge (tGFP-tagged) - Mouse crystallin gamma E (Cryge), (10ug) 10 ug
$500.00
MR219597L3 Lenti ORF clone of Cryge (Myc-DDK-tagged) - Mouse crystallin, gamma E (Cryge) 10 ug
$600.00
MR219597L4 Lenti ORF clone of Cryge (mGFP-tagged) - Mouse crystallin, gamma E (Cryge) 10 ug
$600.00

Plasmid Map for MR219597

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219597
Circular map for MR219597

file-alt Citations

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