Tead4 (NM_011567) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR219506
Tead4 (Myc-DDK-tagged) - Mouse TEA domain family member 4 (Tead4), transcript variant 1
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tead4
Synonyms Etfr; ETFR-; ETFR-2; Etfr2; FR-19; Rtef; Rtef1; Tcf13r; Tcf13r1; TEAD-; TEAD-4; Tef; TEF-3; Tef3; Tefr; Tefr1; Tefr1a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219506 representing NM_011567
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATTACCTCCAACGAGTGGAGCTCTCCCGACTCCCCCGAGGGGAGCAGCATCTCTGGGGGCAGCCAGGCAC
TGGACAAGCCCATCGACAATGATGCAGAGGGTGTATGGAGCCCCGAAATTGAGCGAAGCTTCCAGGAGGC
CCTGGCCATCTACCCACCCTGCGGCCGCCGCAAAATTATCCTGACGGAGGAAGGCAAGATGTATGGTCGG
AATGAGCTGATCGCACGCCATATCAAGCTCAGGACAGGGAAGACGCGCACAAGGAAGCAGGTCTCCAGCC
ACATCCAGGTGCTTGCCCGTCGAAAAGCCCGGGAGATCCAGGCCAAACTCAAGGACCAGGCAGCTAAGAA
CAAGGCCCTGCAGAGCATGGCTGCCATGTCGTCCGCACAGATCGTCTCGGCCACAGCCTTCCACAGTAAA
ATGGCTCTTGCCCGGGGCCCTGGCTACCCAGCAATCTCAGGGTTTTGGCAAGGAGCTTTGCCAGGCCAAC
CTGGAACATCCCACGATGTGAAACCTTTCTCTCAAAACACCTACCCTGTCCAGCCTCCGCTGCCTCTGCC
AGGCTTTGAGTCTCCTGCAGGGCCCACCCCCTCACCCTCCGCACCGCTCGCTCCTCCGTGGCAAGGCCGC
AGCATAGCTAGCTCCAAGCTCTGGATGTTGGAGTTCTCGGCTTTCCTGGAGCGCCAGCAAGATCCCGACA
CATACAACAAACACCTGTTTGTGCACATCAGCCAGTCAAGCCCAAGCTACAGTGACCCCTACCTCGAAAC
TGTGGACATCCGCCAAATCTATGACAAGTTCCCAGAGAAGAAGGGAGGCCTCAAGGAGCTATTTGAACGG
GGGCCCTCTAATGCCTTCTTCCTTGTGAAGTTCTGGGCAGACCTCAATACCAACATCGATGACGAGGGCA
GCGCCTTCTATGGGGTCTCCAGCCAGTACGAGAGCCCGGAGAACATGATTATCACCTGCTCTACGAAGGT
CTGCTCATTTGGCAAGCAAGTGGTGGAGAAAGTTGAGACGGAGTATGCCCGCTATGAGAATGGCCACTAT
TTGTACCGCATCCACCGGTCCCCTCTCTGTGAGTACATGATCAACTTTATCCACAAGCTGAAACACTTAC
CCGAGAAGTACATGATGAACAGTGTGCTGGAGAACTTCACCATCCTGCAGGTGGTCACCAACCGAGACAC
ACAGGAGACCTTGCTGTGTATTGCATATGTCTTCGAAGTTTCAGCCAGCGAGCATGGGGCTCAGCACCAC
ATCTACCGACTTGTGAAAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR219506 representing NM_011567
Red=Cloning site Green=Tags(s)

ITSNEWSSPDSPEGSSISGGSQALDKPIDNDAEGVWSPEIERSFQEALAIYPPCGRRKIILTEEGKMYGR
NELIARHIKLRTGKTRTRKQVSSHIQVLARRKAREIQAKLKDQAAKNKALQSMAAMSSAQIVSATAFHSK
MALARGPGYPAISGFWQGALPGQPGTSHDVKPFSQNTYPVQPPLPLPGFESPAGPTPSPSAPLAPPWQGR
SIASSKLWMLEFSAFLERQQDPDTYNKHLFVHISQSSPSYSDPYLETVDIRQIYDKFPEKKGGLKELFER
GPSNAFFLVKFWADLNTNIDDEGSAFYGVSSQYESPENMIITCSTKVCSFGKQVVEKVETEYARYENGHY
LYRIHRSPLCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTNRDTQETLLCIAYVFEVSASEHGAQHH
IYRLVKE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011567
ORF Size 1281 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 3076 bp
RefSeq ORF 1284 bp
Locus ID 21679
UniProt ID Q62296
Cytogenetics 6 62.92 cM
MW 48 kDa
Summary This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. This factor may play a role in the embryonic development of skeletal muscle. Alternatively spliced transcripts encoding distinct isoforms, which are translated through the use of a non-AUG (AUU) initiation codon, have been described for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_011567" in other vectors (4)
SKU Description Size Price
MC209510 Tead4 (untagged) - Mouse TEA domain family member 4 (Tead4), transcript variant 1, (10ug) 10 ug
$503.00
MG219506 Tead4 (tGFP-tagged) - Mouse TEA domain family member 4 (Tead4) transcript variant 1, (10ug) 10 ug
$657.00
MR219506L3 Lenti ORF clone of Tead4 (Myc-DDK-tagged) - Mouse TEA domain family member 4 (Tead4), transcript variant 1 10 ug
$757.00
MR219506L4 Lenti ORF clone of Tead4 (mGFP-tagged) - Mouse TEA domain family member 4 (Tead4), transcript variant 1 10 ug
$757.00

Plasmid Map for MR219506

Download sequence for

Cloning scheme for MR219506

Circular map for MR219506

file-alt Citations

Platr4 is an early embryonic lncRNA that exerts its function downstream on cardiogenic mesodermal lineage commitment
Hazra, Brine, Garcia et al
Dev Cell (2022) 57 (21), 2450-2468.e7 DOI: 10.1016/j.devcel.2022.10.002
Cited by 17
AI Summary: OriGene products contributed to this study by providing the Tead4 (Cat# MR219506) and Ctgf (Cat# MC200503) mouse gene clones, which were used to investigate the molecular mechanisms by which the lncRNA Platr4 regulates cardiac mesoderm lineage specification.
Snippet: gPCR_Platr4-R2: AGAGACCACCTCCCTCTGCT This paper NA Recombinant DNA Tead4 (NM_011567) Mouse Tagged ORF Clone Origene Cat# MR219506 Ctgf (BC006783) Mouse Untagged Clone Origene Cat#: MC200503
Figure
HIF1α-AS1 is a DNA:DNA:RNA triplex-forming lncRNA interacting with the HUSH complex
Leisegang, Bains, Seredinski et al
Nat Commun (2022) 13 (1), 6563 DOI: 10.1038/s41467-022-34252-2
Cited by 49
AI Summary: OriGene products contributed to this study by providing the pCMV6-MPP8-MYC-DDK plasmid (Cat# RC202562L3), the Recombinant DNA Tead4 Mouse Tagged ORF Clone (Cat# MR219506), and the Ctgf Mouse Untagged Clone (Cat# MC200503), which were used for gene expression and functional analyses related to the role of HIF1α-AS1 in DNA:DNA:RNA triplex formation and gene regulation.
Snippet: … pCMV6-MPP8-MYC-DDK was obtained from Origene (#RC202562L3). The plasmid pcDNA3.1 + HIF1α-AS1_1200 (called TFR2) included the first 1200 nt (hg19, chr14:62,161,342-62,162,541) of the genomic DNA of the HIF1α-AS1 gene and was synthes;gPCR_Platr4-R2: AGAGACCACCTCCCTCTGCT This paper NA Recombinant DNA Tead4 (NM_011567) Mouse Tagged ORF Clone Origene Cat# MR219506 Ctgf (BC006783) Mouse Untagged Clone Origene Cat#: MC200503
FigureFigureFigure
Glycolysis-Independent Glucose Metabolism Distinguishes TE from ICM Fate during Mammalian Embryogenesis
Chi, Sharpley, Nagaraj et al
Dev. Cell (2020) 53 (1), 9-26.e4 DOI: 10.1016/j.devcel.2020.02.015
Cited by 197
AI Summary: In this study, OriGene products pCMV6-Yap1, pCMV6-Tead4, and pCMV6-Tfap2c were used to express key transcription factors, enabling the investigation of their roles in trophectoderm (TE) fate specification during early mammalian embryogenesis.
Snippet: Skip to article;pGEMHE-mCherry-mTrim21 Addgene Cat #105522 pCMV6-Yap1 OriGene Cat #MR226049 pCMV6-Tead4 (ATT) OriGene Cat #MR219506 pCMV6-Tfap2c OriGene Cat #MR207174 pCMV6-Flag-Yap1 This paper N/A pCMV6-Myc-Tead4 (ATG) This paper N/A pQCMV-GFP-Tfap2c This paper N/A
Metabolic Regulation of Preimplantation Mouse Embryo Development
Chi
Thesis (2020)
Snippet: pGEMHE-mCherry-mTrim21 Addgene Cat #105522 pCMV6-Yap1 OriGene Cat #MR226049 pCMV6-Tead4 (ATT) OriGene Cat #MR219506 pCMV6-Tfap2c OriGene Cat #MR207174 pCMV6-Flag-Yap1 This lab N/A pCMV6-Myc-Tead4 (ATG) This lab N/A pQCMV-GFP-Tfap2c This lab N/A
Glucose metabolism distinguishes TE from ICM fate during mammalian embryogenesis
Chi, Sharpley, Nagaraj et al
bioRxiv (2019)
Snippet: GFP mRNA (TriLink #L-7201), pGEMHE-mCherry-mTrim21 (Addgene #105522), pCMV6-YAP1 (OriGene #MR219506), pCMV6-TEAD4 (OriGene #MR219506) and pCMV6-AP-2γ (Origene #MR207174)
Showing 1-5 of 5 papers.
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