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Cnn1 (NM_009922) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219445
Cnn1 (Myc-DDK-tagged) - Mouse calponin 1 (Cnn1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cnn1
Synonyms CN; CnnI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219445 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTCTGCACATTTTAACCGAGGTCCTGCCTACGGCTTGTCTGCTGAAGTAAAGAACAAGCTGGCCC
AGAAATACGACCATCAGCGGGAGCAGGAGCTGAGAGAGTGGATTGAGGGGGTGACAGGCCGGCGCATCGG
GAACAACTTCATGGATGGCCTCAAAGATGGGATCATTCTTTGCGAATTTATCAACAAGCTGCAGCCGGGT
TCTGTGAAGAAGGTCAATGAGTCAACTCAGAACTGGCACCAGCTGGAGAACATAGGTAATTTCATCAAAG
CCATTACCAAGTATGGGGTGAAACCCCACGACATCTTTGAGGCCAACGACCTGTTTGAAAACACCAACCA
TACACAAGTTCAGTCCACTCTCCTGGCTCTGGCCAGCATGGCCAAGACAAAAGGAAACAAAGTCAATGTG
GGAGTCAAGTATGCAGAGAAACAAGAGCGGAGATTTGAGCCGGAGAAGTTGAGAGAAGGCAGGAACATCA
TTGGACTGCAGATGGGCACCAACAAGTTTGCCAGTCAGCAGGGCATGACGGCCTATGGTACACGGCGTCA
CCTCTATGATCCCAAACTGGGTACAGATCAGCCTCTGGACCAGGCGACCATCAGCCTGCAGATGGGCACC
AACAAGGGTGCCAGCCAGGCTGGCATGACTGCACCAGGCACCAAGCGGCAGATCTTTGAGCCAGGTCTGG
GCATGGAACACTGCGACACACTCAACGTCAGCTTGCAGATGGGCAGCAACAAGGGGGCCTCCCAGAGGGG
CATGACAGTGTATGGGCTTCCTCGCCAGGTGTACGATCCCAAGTACTGCCTGAACCCGGAGTACCCAGAG
CTGAGTGAGCCCACCCACAATCACCACCCGCACAACTACTACAACTCTGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219445 protein sequence
Red=Cloning site Green=Tags(s)

MSSAHFNRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIGNNFMDGLKDGIILCEFINKLQPG
SVKKVNESTQNWHQLENIGNFIKAITKYGVKPHDIFEANDLFENTNHTQVQSTLLALASMAKTKGNKVNV
GVKYAEKQERRFEPEKLREGRNIIGLQMGTNKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGT
NKGASQAGMTAPGTKRQIFEPGLGMEHCDTLNVSLQMGSNKGASQRGMTVYGLPRQVYDPKYCLNPEYPE
LSEPTHNHHPHNYYNSA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009922
ORF Size 891 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009922.1, NM_009922.2, NM_009922.3, NM_009922.4, NP_034052.3
RefSeq Size 1987 bp
RefSeq ORF 894 bp
Locus ID 12797
UniProt ID Q08091
Cytogenetics 9 A3
MW 33.4 kDa
Summary Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.UniProtKB/Swiss-Prot Function
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"NM_009922" in other vectors (4)
SKU Description Size Price
MC208305 Cnn1 (untagged) - Mouse calponin 1 (Cnn1), (10ug) 10 ug
$330.00
MG219445 Cnn1 (tGFP-tagged) - Mouse calponin 1 (Cnn1), (10ug) 10 ug
$500.00
MR219445L3 Lenti ORF clone of Cnn1 (Myc-DDK-tagged) - Mouse calponin 1 (Cnn1) 10 ug
$600.00
MR219445L4 Lenti ORF clone of Cnn1 (mGFP-tagged) - Mouse calponin 1 (Cnn1) 10 ug
$600.00

Plasmid Map for MR219445

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219445
Circular map for MR219445

file-alt Citations

Inhibiting Monoacylglycerol Lipase Suppresses RANKL-Induced Osteoclastogenesis and Alleviates Ovariectomy-Induced Bone Loss
Liu, Zhou, Qi et al
Front Cell Dev Biol (2021) 9, 640867 DOI: 10.3389/fcell.2021.640867
Cited by 8
AI Summary: OriGene Technologies contributed DDK-tagged H1 calponin (CNN1) plasmids and a control plasmid, which were used to assess the effects of H1 calponin overexpression on osteoclastogenesis in the study.
Snippet: at the density of 4 × 10 4 cells/well the day before transfection and infected with the virus for 12 h. Expression changes in MAGL were detected after 48 h. DDK-tagged H1 calponin (CNN1) plasmids and the control plasmid were obtained from OriGene Technologies Cached
FigureFigureFigure
Showing 1-1 of 1 papers.
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