Il10ra (NM_008348) Mouse Tagged ORF Clone

SKU MR219426

Il10ra (Myc-DDK-tagged) - Mouse interleukin 10 receptor, alpha (Il10ra)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Il10ra
• Synonyms: AW553859; CDw210; CDw210a; IL-10R1; IL-10RA; Il10r; mIL-10R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219426 representing NM_008348; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTGTCGCGTTTGCTCCCATTCCTCGTCACGATCTCCAGCCTGAGCCTAGAATTCATTGCATACGGGA; CAGAACTGCCAAGCCCTTCCTATGTGTGGTTTGAAGCCAGATTTTTCCAGCACATCCTCCACTGGAAACC; TATCCCAAACCAGTCTGAGAGCACCTACTATGAAGTGGCCCTCAAACAGTACGGAAACTCAACCTGGAAT; GACATCCATATCTGTAGAAAGGCTCAGGCATTGTCCTGTGATCTCACAACGTTCACCCTGGATCTGTATC; ACCGAAGCTATGGCTACCGGGCCAGAGTCCGGGCAGTGGACAACAGTCAGTACTCCAACTGGACCACCAC; TGAGACTCGCTTCACAGTGGATGAAGTGATTCTGACAGTGGATAGCGTGACTCTGAAAGCAATGGACGGC; ATCATCTATGGGACAATCCATCCCCCCAGGCCCACGATAACCCCTGCAGGGGATGAGTACGAACAAGTCT; TCAAGGATCTCCGAGTTTACAAGATTTCCATCCGGAAGTTCTCAGAACTAAAGAATGCAACCAAGAGAGT; GAAACAGGAAACCTTCACCCTCACGGTCCCCATAGGGGTGAGAAAGTTTTGTGTCAAGGTGCTGCCCCGC; TTGGAATCCCGAATTAACAAGGCAGAGTGGTCGGAGGAGCAGTGTTTACTTATCACGACGGAGCAGTATT; TCACTGTGACCAACCTGAGCATCTTAGTCATATCTATGCTGCTATTCTGTGGAATCCTGGTCTGTCTGGT; TCTCCAGTGGTACATCCGGCACCCGGGGAAGTTGCCTACAGTCCTGGTCTTCAAGAAGCCTCACGACTTC; TTCCCAGCCAACCCTCTCTGCCCAGAAACTCCCGATGCCATTCACATCGTGGACCTGGAGGTTTTCCCAA; AGGTGTCACTAGAGCTGAGAGACTCAGTCCTGCATGGCAGCACCGACAGTGGCTTTGGCAGTGGTAAACC; ATCACTTCAGACTGAAGAGTCCCAATTCCTCCTCCCTGGCTCCCACCCCCAGATACAGGGGACTCTGGGA; AAAGAAGAGTCTCCAGGGCTACAGGCCACCTGTGGGGACAACACGGACAGTGGGATCTGCCTGCAGGAGC; CCGGCTTACACTCCAGCATGGGGCCCGCCTGGAAGCAGCAGCTTGGATATACCCATCAGGACCAGGATGA; CAGTGACGTTAACCTAGTCCAGAACTCTCCAGGGCAGCCTAAGTACACACAGGATGCATCTGCCTTGGGC; CATGTCTGTCTCCTAGAACCTAAAGCCCCTGAGGAGAAAGACCAAGTCATGGTGACATTCCAGGGCTACC; AGAAACAGACCAGATGGAAGGCAGAGGCAGCAGGCCCAGCAGAATGCTTGGACGAAGAGATTCCCTTGAC; AGATGCCTTTGATCCTGAACTTGGGGTACACCTGCAGGATGATTTGGCTTGGCCTCCACCAGCTCTGGCC; GCAGGTTATTTGAAACAGGAGTCTCAAGGGATGGCTTCTGCTCCACCAGGGACACCAAGTAGACAGTGGA; ATCAACTGACCGAAGAGTGGTCACTCCTGGGTGTGGTTAGCTGTGAAGATCTAAGCATAGAAAGTTGGAG; GTTTGCCCATAAACTTGACCCTCTGGACTGTGGGGCAGCCCCTGGTGGCCTCCTGGATAGCCTTGGCTCT; AACCTGGTCACCCTGCCGTTGATCTCCAGCCTGCAGGTAGAAGAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219426 representing NM_008348; Red=Cloning site Green=Tags(s); ; MLSRLLPFLVTISSLSLEFIAYGTELPSPSYVWFEARFFQHILHWKPIPNQSESTYYEVALKQYGNSTWN; DIHICRKAQALSCDLTTFTLDLYHRSYGYRARVRAVDNSQYSNWTTTETRFTVDEVILTVDSVTLKAMDG; IIYGTIHPPRPTITPAGDEYEQVFKDLRVYKISIRKFSELKNATKRVKQETFTLTVPIGVRKFCVKVLPR; LESRINKAEWSEEQCLLITTEQYFTVTNLSILVISMLLFCGILVCLVLQWYIRHPGKLPTVLVFKKPHDF; FPANPLCPETPDAIHIVDLEVFPKVSLELRDSVLHGSTDSGFGSGKPSLQTEESQFLLPGSHPQIQGTLG; KEESPGLQATCGDNTDSGICLQEPGLHSSMGPAWKQQLGYTHQDQDDSDVNLVQNSPGQPKYTQDASALG; HVCLLEPKAPEEKDQVMVTFQGYQKQTRWKAEAAGPAECLDEEIPLTDAFDPELGVHLQDDLAWPPPALA; AGYLKQESQGMASAPPGTPSRQWNQLTEEWSLLGVVSCEDLSIESWRFAHKLDPLDCGAAPGGLLDSLGS; NLVTLPLISSLQVEE; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008348
• ORF Size: 1725 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_008348.3
• RefSeq Size: 3493 bp
• RefSeq ORF: 1728 bp
• Locus ID: 16154
• UniProt ID: Q61727
• Cytogenetics: 9 24.84 cM
• MW: 64.7 kDa
• Summary: Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3 (PubMed:8910398). Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (By similarity).[UniProtKB/Swiss-Prot Function]