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Brox (NM_027861) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219392
Brox (Myc-DDK-tagged) - Mouse BRO1 domain and CAAX motif containing (Brox)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Brox
Synonyms 0610010K06Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219392 representing NM_027861
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCATTGGTTTCATAGGAACCCATTAAAAGCTACAGCTCCCGTGTCTTTTAACTACTATGGCATGA
TCACTGGTCCTCCCGCTTCAAAGATTTGCAATGACTTGAGGTCCGCCAGGACTCGGCTCCTCGAGTTGTT
CACTGATTTGAGCTGTAATCCAGAAACAATGAAGAATGCGGCAGACTTGTATTTCTCTCTTTTACAAGGT
TTCATAAATTCAGTGGGTGACTCTACACAGGAAAGCAAATTACGATACATTCAGAATTTCAAGTGGACCG
ATACATTGCAAGGACACGTTCCGAGTGCCCAGCAGGATGCTGTTTTTGAACTGATTTCCATGGGATTTAA
TGTAGCGCTGTGGTACACAAAATATGCTTCAAGATTGGCTGGAAAAGAAAACATAACAGAAGATGAAGCA
AAAGAAGTTCACCGAAGCCTAAAAATTGCAGCTGGGATTTTTAAACATTTAAAGGAAAGTCATATCCCCA
AACTTCTTACACCAGCAGAAAAGGGGCGGGACTTGGAGGCTCGGCTCATCGATGCTTACATCATCCAGTG
CCAGGCCGAGGCTCAGGAAGTGACAATCGCCCGAGCCATTGAGCTGAAGCACGCTCCGGGACTGATCGCT
GCACTCGCCTATGACACAGCCAGCTTCTACCAGAAAGCAGATCATACTTTGTCCAGTTTGGAGCCTGCAC
ACTCTGCTAAATGGAGAAAGTACCTTCACTTGAAGATGTGCTTCTACACAGCGTATGCGTATTGCTACCA
TGGCCAGACTCTGTTGGCGAGCGATAAGTGTGGAGAAGCCATCCGATCTCTCCAAGAAGCAGAAAAATTG
TACGCAGAGGCAGAAGCCCTATGCAAAGAGTACGGGGAAACCAAAGGACCAGGGCCGACGGCCAAGCCGT
CAGGACACTTGTTCTTCCGGAAGCTTGGGAGCCTTGTGAAGAACACCCTGGACAAATGTCAGAGAGAAAA
TGGCTTCATTTACTTTCAAAAAATTCCAACAGAAGCCCCACAACTGGAACTCAAAGCAAATTATGGGCTG
GTAGAGCCTGTGCCTTTTGAATTCCCTCCTATGAGTGCTCACTGGACACCGGAAGCGTTGGCTGCGTTTG
ATCTCACCAAGAGACCCAAGGATGACAGTGTCAAACCCAAACCGGAAGAGGACGTGAAACCTGTAAAGGA
ACCAGATATCAGACCTCAGAAGGACACTGGGTGCTCGGTCTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219392 representing NM_027861
Red=Cloning site Green=Tags(s)

MTHWFHRNPLKATAPVSFNYYGMITGPPASKICNDLRSARTRLLELFTDLSCNPETMKNAADLYFSLLQG
FINSVGDSTQESKLRYIQNFKWTDTLQGHVPSAQQDAVFELISMGFNVALWYTKYASRLAGKENITEDEA
KEVHRSLKIAAGIFKHLKESHIPKLLTPAEKGRDLEARLIDAYIIQCQAEAQEVTIARAIELKHAPGLIA
ALAYDTASFYQKADHTLSSLEPAHSAKWRKYLHLKMCFYTAYAYCYHGQTLLASDKCGEAIRSLQEAEKL
YAEAEALCKEYGETKGPGPTAKPSGHLFFRKLGSLVKNTLDKCQRENGFIYFQKIPTEAPQLELKANYGL
VEPVPFEFPPMSAHWTPEALAAFDLTKRPKDDSVKPKPEEDVKPVKEPDIRPQKDTGCSVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027861
ORF Size 1233 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027861.4
RefSeq Size 3457 bp
RefSeq ORF 1236 bp
Locus ID 71678
UniProt ID Q8K2Q7
Cytogenetics 1 H5
MW 46.2 kDa
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Documents
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"NM_027861" in other vectors (4)
SKU Description Size Price
MC211213 Brox (untagged) - Mouse BRO1 domain and CAAX motif containing (Brox), (10ug) 10 ug
$503.00
MG219392 Brox (tGFP-tagged) - Mouse RIKEN cDNA 0610010K06 gene (0610010K06Rik), (10ug) 10 ug
$657.00
MR219392L3 Lenti ORF clone of Brox (Myc-DDK-tagged) - Mouse BRO1 domain and CAAX motif containing (Brox) 10 ug
$757.00
MR219392L4 Lenti ORF clone of Brox (mGFP-tagged) - Mouse BRO1 domain and CAAX motif containing (Brox) 10 ug
$757.00

Plasmid Map for MR219392

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219392
Circular map for MR219392

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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