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Me3 (NM_181407) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219248
Me3 (Myc-DDK-tagged) - Mouse malic enzyme 3, NADP(+)-dependent, mitochondrial (Me3), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $563.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Me3
Synonyms 1700020C08Rik; B230207H15Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219248 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGCCGCGCTGGGCACTGGCGCGCGGCTGACCTCCGTGCCTCGCATAGCCTGCAGCAGTCTTCGGC
GCCAGGCACCTTCCGCGCCCGCCCAGGGCTGCCACTCCAAGTCTGGCCCGCCTCGACCCGTACCCCTGAA
GAAGCGCGGATACGATGTCACCAGGAACCCCCATCTCAACAAGGGCATGGCCTTTACACTTGAAGAGAGG
CTGCAGCTCGGCATCCACGGCCTGATCCCCCCTTGCTTCCTCAGCCAGGATGTGCAGCTCCTCCGCATCA
TGAGATACTATGAGAATCAGCAGAGCGACCTGGACAAGTACATCATTCTCATGACTCTCCAAGACCGCAA
CGAGAAGCTCTTCTACCGGGTGCTGACCTCAGACGTGGAGAAGTTCATGCCCATTGTGTACACACCCACT
GTGGGGCTGGCCTGTCAGCACTACGGCCTCACCTTCCGCCGACCCCGTGGGCTGTTCATCACCATTCATG
ACAAGGGCCACATTGCAACAATGCTGAACTCTTGGCCAGAAGACAATATTAAGGCTGTGGTGGTGACTGA
TGGAGAGCGCATACTGGGCTTGGGAGACCTGGGCTGCTACGGCATGGGCATCCCTGTGGGCAAGCTGGCC
CTGTACACAGCATGTGGAGGGGTGAATCCACAGCAGTGCCTTCCTGTCCTGCTAGATGTTGGCACCAACA
ATGAGGAGCTTCTTAGAGACCCTCTGTACATCGGCCTGAAACACCAGCGTGTCCGTGGGGAGGAGTATGA
TGACCTGCTGGATGAATTCATGCAGGCGGTGACAGATAAGTTCGGAATAAATTGCCTCATCCAGTTCGAA
GACTTTGCCAATGCCAATGCCTTCCGTCTACTCAACAAATACCGCAACAAGTACTGTATGTTCAATGATG
ACATCCAAGGCACAGCCTCTGTCGCTGTGGCAGGGATCCTGGCTGCTCTGAGAATCACCAAGAACAGGCT
GTCCAATCATGTGTTTGTTTTCCAGGGTGCGGGGGAGGCAGCCATGGGCATTGCCCACCTCCTTGTCATG
GCCCTGGAAAAGGAAGGCATACCCAAGACAGAAGCCATAAAGAAGATCTGGATGGTGGACTCCAAGGGTC
TGATTGTCAAGGGAAGAAGCCACCTAAACCATGAGAAGGAGATGTTTGCCCAAGACCACCCTGAAGTCAA
CTCCCTGGAGGAGGTAGTAAGGCTGGTGAAGCCCACAGCAATTATAGGTGTCGCTGCCATCGCAGGAGCC
TTCACGGAGCAGATTCTGAGGGACATGGCTTCCTTCCATGAGCGCCCTATCGTCTTTGCCCTGAGCAACC
CCACCAGCAAGGCTGAGTGCACAGCTGAGAAGTGCTACCGGGTCACCGAGGGCCGAGGAATCTTTGCCAG
TGGAAGTCCTTTTAAGAGTGTGACTCTGGAAGATGGCAGGACATTCACTCCTGGTCAGGGAAACAATGCC
TATGTGTTTCCTGGGGTAGCTCTGGGTGTCATCGCTGGTGGGATCCGGCACATCCCAGATGAAATCTTCC
TCCTGACGGCAGAGCAAATTGCCCAAGAAGTCTCTGAGCAGCACCTGTCTCAGGGGAGACTCTACCCACC
ACTCAGCACCATCCGAGATGTGTCTCTGAGAATTGCAGTCAAAGTCCTCGACTATGCATACAAACACAAC
TTGGCCTCCTACTATCCAGAGCCCAAGGACAAGGAGGCTTTTGTAAAATCTCTGATCTACACTCCAGACT
ATGACTCCTTCTCACTAGACACCTACAGCTGGCCTAAGGAAGCTATGAGTGTTCAGAAGGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219248 protein sequence
Red=Cloning site Green=Tags(s)

MGAALGTGARLTSVPRIACSSLRRQAPSAPAQGCHSKSGPPRPVPLKKRGYDVTRNPHLNKGMAFTLEER
LQLGIHGLIPPCFLSQDVQLLRIMRYYENQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPT
VGLACQHYGLTFRRPRGLFITIHDKGHIATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLA
LYTACGGVNPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVRGEEYDDLLDEFMQAVTDKFGINCLIQFE
DFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNRLSNHVFVFQGAGEAAMGIAHLLVM
ALEKEGIPKTEAIKKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGA
FTEQILRDMASFHERPIVFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGRTFTPGQGNNA
YVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAVKVLDYAYKHN
LASYYPEPKDKEAFVKSLIYTPDYDSFSLDTYSWPKEAMSVQKV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181407
ORF Size 1812 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181407.1, NM_181407.2, NM_181407.3, NM_181407.4, NP_852072.2
RefSeq Size 4370 bp
RefSeq ORF 1815 bp
Locus ID 109264
UniProt ID Q8BMF3
Cytogenetics 7 D3
MW 67.1 kDa
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Documents
Resources
"NM_181407" in other vectors (4)
SKU Description Size Price
MC219646 Me3 (untagged) - Mouse malic enzyme 3, NADP(+)-dependent, mitochondrial (Me3), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$619.00
MG219248 Me3 (tGFP-tagged) - Mouse malic enzyme 3 NADP(+)-dependent mitochondrial (Me3) nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$763.00
MR219248L3 Lenti ORF clone of Me3 (Myc-DDK-tagged) - Mouse malic enzyme 3, NADP(+)-dependent, mitochondrial (Me3), nuclear gene encoding mitochondrial protein 10 ug
$863.00
MR219248L4 Lenti ORF clone of Me3 (mGFP-tagged) - Mouse malic enzyme 3, NADP(+)-dependent, mitochondrial (Me3), nuclear gene encoding mitochondrial protein 10 ug
$863.00

Plasmid Map for MR219248

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219248
Circular map for MR219248

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