Ulk3 (NM_027895) Mouse Tagged ORF Clone

SKU MR219200

Ulk3 (Myc-DDK-tagged) - Mouse unc-51-like kinase 3 (C. elegans) (Ulk3)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Ulk3
• Synonyms: 1200015E14Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219200 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTGGGCCCAGCTGGGGTCTCCCTCGGCTGGACGGTTTCATCCTTACCGAGCGCCTGGGCAGTGGCA; CGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGATACTCGGGAAGTGGTAGCCATAAAATGCGTGGC; CAAGAAGAGTCTCAACAAGGCGTCAGTGGAAAACCTCCTGACTGAGATTGAGATCCTCAAGGGCATTCGG; CACCCCCATATCGTGCAGCTGAAAGACTTCCAGTGGGACAATGACAATATCTACCTCATCATGGAGTTCT; GTGCAGGGGGTGACCTGTCTCGCTTCATTCATACCCGCAGGATCCTGCCTGAGAAGGTGGCCCGTGTTTT; CATGCAGCAGTTGGCTAGTGCCCTGCAGTTCCTGCATGAACGAAACATCTCTCACTTGGATCTGAAACCG; CAGAACATCCTGCTGAGCTCTTTGGAGAAGCCCCACCTGAAACTGGCAGACTTTGGCTTTGCCCAGCACA; TGTCCCCGTGGGACGAAAAACACGTGCTCCGTGGCTCCCCGCTCTATATGGCTCCTGAGATGGTGTGTCG; GCGGCAGTATGATGCGCGTGTGGACCTCTGGTCTGTGGGGGTGATCCTGTACGAAGCCCTCTTTGGGCAG; CCCCCCTTTGCCTCCAGATCGTTCTCAGAGCTAGAAGAAAAGATTCGCAGCAATCGGGTGATTGAGCTCC; CTCTTCGGCCCCAACTCTCCCTAGACTGCCGGGACCTGTTGCAGCGACTTCTAGAGCGGGACCCCGCCCG; TCGAATCTCCTTCAAGGACTTCTTTGCCCATCCCTGGGTGGACCTGGAGCACATGCCCAGTGGGGAGAGC; CTGGCACAGGCAAGGGCCCTTGTGGTGGAGGCTGTGAAGAAGGACCAGGAGGGGGATGCTGCCGCTGCCC; TGTCGCTCTACTGCAAGGCTCTGGACTTCTTTGTACCTGCGCTACACTACGAAGTGGATGCCCAGAGGAA; GGAGGCAATTAAGGCGAAGGTGGGACAGTATGTGTCCCGGGCAGAGGAGCTCAAAGCCATTGTCTCCTCC; TCCAATCAGGCCCTGCTAAGACAGGGCACAACTGTCCAAGAGCTGCTTCGAGAGATGGCCCGTGACAAAC; CACGCCTCCTGGCTGCCCTGGAAGTGGCCTCAGCTGCCCTGGCCAAGGAGGAGGAAGCTGGCAAAGAGCA; GGATGCCCTGGACCTGTACCAGCACAGCCTCGGGGAGCTGCTAGTGCTGTTGGCAGCAGAGGCCCCAGGC; CGAAGGCGGGAGCTCCTTCACACCGAGGTTCAGAACCTCATGGCTCGAGCTGAATACCTGAAGGAGCAGA; TCAAGATAAGGGAGTCTCACTGGGAAGCGGAGAGTCTGGACAAAGAGGGGCTGTCGGAGTCTGTTCGTAG; TTCTTGCACACTGCAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219200 protein sequence; Red=Cloning site Green=Tags(s); ; MAGPSWGLPRLDGFILTERLGSGTYATVYKAYAKKDTREVVAIKCVAKKSLNKASVENLLTEIEILKGIR; HPHIVQLKDFQWDNDNIYLIMEFCAGGDLSRFIHTRRILPEKVARVFMQQLASALQFLHERNISHLDLKP; QNILLSSLEKPHLKLADFGFAQHMSPWDEKHVLRGSPLYMAPEMVCRRQYDARVDLWSVGVILYEALFGQ; PPFASRSFSELEEKIRSNRVIELPLRPQLSLDCRDLLQRLLERDPARRISFKDFFAHPWVDLEHMPSGES; LAQARALVVEAVKKDQEGDAAAALSLYCKALDFFVPALHYEVDAQRKEAIKAKVGQYVSRAEELKAIVSS; SNQALLRQGTTVQELLREMARDKPRLLAALEVASAALAKEEEAGKEQDALDLYQHSLGELLVLLAAEAPG; RRRELLHTEVQNLMARAEYLKEQIKIRESHWEAESLDKEGLSESVRSSCTLQ; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027895
• ORF Size: 1416 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_027895.1, NP_082171.1
• RefSeq Size: 2826 bp
• RefSeq ORF: 1419 bp
• Locus ID: 71742
• UniProt ID: Q3U3Q1
• Cytogenetics: 9 B
• MW: 53.6 kDa
• Summary: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy (By similarity).[UniProtKB/Swiss-Prot Function]