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Dnajc22 (NM_176835) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219159
Dnajc22 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 22 (Dnajc22)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dnajc22
Synonyms 2810451A06Rik; AI506245
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219159 representing NM_176835
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAAAGGGCTGCTGATGACCTATGTCCTTTGGGCTTTGGGGGGTCCTGTTGGGCTCCACCACCTGT
ACCTGGGAAGGGACAGCCATGCCCTGCTCTGGATGCTCACCCTGGGAGGTGGTGGGTTGGGCTGGCTCTG
GGAATTCTGGAAGCTCCCGAGCTTTGTGGCTCAAGCCAACAGAGTCCAGAGTTGGAAACAGAGGCCGGAA
GAGGAGAGACCACCTCTCAGTCTCCTTCGCTTTGCTTCCCAGATAGTAGTGGGTGTCTATTTTGGTCTTG
TGGCTCTAGTCAGCCTTTCTTCCATGGACAACTTCTATATAGTGGGCCTCCCACTGGCTGTTGGCTTAGG
GGTCTTGCTGGTAGCTGCTGTTGGTAACCAGACCTCAGACTTTAAGAATACCTTAGGAGCGGCCTTCCTC
ACGTCCCCTGTGTTCTATGGCCGCCCCATAGCCATCCTGCCTATCAGTTTGGCTGCCAGCATTACAGCCC
AGAAGCATCGACGATACAAAGCCTCAGCGGGGTCAGAGACACTTAGTGTGCGGCTCTACCGCGTGGGCTT
GGCTTACCTCGCCTTCACCGGCCCTCTGGCCTACAGTACGATGTACAACACAGCTGCCACCATTAACTAC
GTGGCAGAAACCCTGGGTTCCTTCTTGAGTTGGTTCAGCTTTTTCCCACTTCTTGGCCGCCTCGTGGAGT
CTGTCTTGCTTCTGCCTTGCCGGATCTGGTGGCTACTGGTCGGAGGTCCTGGCTTCAACAGCAGCCGGTT
CCAGGAGTGGGAAAAGCTCTATGAGTTTGTCGACAGTTTTCAGGATGAGAAACTTCAGCTGGCTCACCAG
GTTCTGGGCGTCCCAGAAGGAGCAACTAATGAAGAAATACATCGGAGTTACCGGGACCTGGTGAAAGTCT
GGCACCCAGATCACAACCGGCACCAGACGGAGGAGGCCCAGAGACACTTCCTGGAGATCCAGGCTGCGTA
TGAAGTCTTGAGTCAGCCCAAGAAGCCTAGAGCATCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219159 representing NM_176835
Red=Cloning site Green=Tags(s)

MAKGLLMTYVLWALGGPVGLHHLYLGRDSHALLWMLTLGGGGLGWLWEFWKLPSFVAQANRVQSWKQRPE
EERPPLSLLRFASQIVVGVYFGLVALVSLSSMDNFYIVGLPLAVGLGVLLVAAVGNQTSDFKNTLGAAFL
TSPVFYGRPIAILPISLAASITAQKHRRYKASAGSETLSVRLYRVGLAYLAFTGPLAYSTMYNTAATINY
VAETLGSFLSWFSFFPLLGRLVESVLLLPCRIWWLLVGGPGFNSSRFQEWEKLYEFVDSFQDEKLQLAHQ
VLGVPEGATNEEIHRSYRDLVKVWHPDHNRHQTEEAQRHFLEIQAAYEVLSQPKKPRAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_176835
ORF Size 1017 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_176835.2, NP_789805.1
RefSeq Size 1475 bp
RefSeq ORF 1020 bp
Locus ID 72778
UniProt ID Q8CHS2
Cytogenetics 15 F1
MW 38.4 kDa
Summary May function as a co-chaperone.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_176835" in other vectors (4)
SKU Description Size Price
MC211356 Dnajc22 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 22 (Dnajc22), (10ug) 10 ug
$503.00
MG219159 Dnajc22 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog subfamily C member 22 (Dnajc22), (10ug) 10 ug
$657.00
MR219159L3 Lenti ORF clone of Dnajc22 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 22 (Dnajc22) 10 ug
$757.00
MR219159L4 Lenti ORF clone of Dnajc22 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 22 (Dnajc22) 10 ug
$757.00

Plasmid Map for MR219159

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219159
Circular map for MR219159

file-alt Citations

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