OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Mcu (NM_001033259) Mouse Tagged Lenti ORF Clone

  • LentiORF®
Be the first to review this product
SKU
MR218926L3
Lenti ORF clone of Mcu (Myc-DDK-tagged) - Mouse coiled-coil domain containing 109A (Ccdc109a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $757.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA363360 MCU Antibody - C-terminal region
100 ul
$585.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00
GE100111 Forward primer to sequence targets cloned in expression vectors.
1 nmol
$71.00
GE100128 Reverse primer to sequence targets cloned in expression vectors.
1 nmol
$71.00
TR30037 Lenti-vpak packaging kit - packaging plasmids and transfection reagent
10 reactions
$720.00

Other products for "Mcu or NM_001033259"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mcu
Synonyms 2010012O16Rik; AV064928; C10orf42; Ccdc109a; D130073L02Rik; Gm64
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR218926).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033259
ORF Size 1050 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033259.3
RefSeq Size 1423 bp
RefSeq ORF 1053 bp
Locus ID 215999
UniProt ID Q3UMR5
Cytogenetics 10 B4
Summary Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria (PubMed:21685886, PubMed:23900286, PubMed:24212091). Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex) (By similarity). Activity is regulated by MICU1 and MICU2 (By similarity). At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity (By similarity). Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways (By similarity). Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes (By similarity). While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells (PubMed:26119742, PubMed:26119731, PubMed:25603276). Participates in mitochondrial permeability transition during ischemia-reperfusion injury (PubMed:26119731). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (By similarity). Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults (PubMed:25732818). Regulates synaptic vesicle endocytosis kinetics in central nerve terminal (PubMed:26644474). Involved in antigen processing and presentation (PubMed:25251370).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001033259" in other vectors (4)
SKU Description Size Price
MC212635 Mcu (untagged) - Mouse coiled-coil domain containing 109A (Ccdc109a), (10ug) 10 ug
$457.00
MG218926 Mcu (tGFP-tagged) - Mouse coiled-coil domain containing 109A (Ccdc109a), (10ug) 10 ug
$489.00 $657.00
MR218926 Mcu (Myc-DDK-tagged) - Mouse coiled-coil domain containing 109A (Ccdc109a) 10 ug
$289.00 $457.00
MR218926L4 Lenti ORF clone of Mcu (mGFP-tagged) - Mouse coiled-coil domain containing 109A (Ccdc109a) 10 ug
$757.00

Plasmid Map for MR218926L3

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218926L3
Circular map for MR218926L3

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products