Mcu (NM_001033259) Mouse Untagged Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Mcu |
| Synonyms | 2010012O16Rik; AV064928; C10orf42; Ccdc109a; D130073L02Rik; Gm64 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI | |
| ACCN | NM_001033259 |
| Insert Size | 1053 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001033259.3, NP_001028431.2 |
| RefSeq Size | 1423 bp |
| RefSeq ORF | 1053 bp |
| Locus ID | 215999 |
| UniProt ID | Q3UMR5 |
| Cytogenetics | 10 B4 |
| Summary | Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria (PubMed:21685886, PubMed:23900286, PubMed:24212091). Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex) (By similarity). Activity is regulated by MICU1 and MICU2 (By similarity). At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity (By similarity). Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways (By similarity). Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes (By similarity). While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells (PubMed:26119742, PubMed:26119731, PubMed:25603276). Participates in mitochondrial permeability transition during ischemia-reperfusion injury (PubMed:26119731). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (By similarity). Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults (PubMed:25732818). Regulates synaptic vesicle endocytosis kinetics in central nerve terminal (PubMed:26644474). Involved in antigen processing and presentation (PubMed:25251370).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001033259" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG218926 | Mcu (tGFP-tagged) - Mouse coiled-coil domain containing 109A (Ccdc109a), (10ug) | 10 ug |
$489.00
$657.00
|
|
| MR218926 | Mcu (Myc-DDK-tagged) - Mouse coiled-coil domain containing 109A (Ccdc109a) | 10 ug |
$289.00
$457.00
|
|
| MR218926L3 | Lenti ORF clone of Mcu (Myc-DDK-tagged) - Mouse coiled-coil domain containing 109A (Ccdc109a) | 10 ug |
$757.00
|
|
| MR218926L4 | Lenti ORF clone of Mcu (mGFP-tagged) - Mouse coiled-coil domain containing 109A (Ccdc109a) | 10 ug |
$757.00
|
|
Citations
The mitochondrial calcium uniporter (MCU) activates mitochondrial respiration and enhances mobility by regulating mitochondrial redox state
Weiser, Hermant, Bermont et al
Redox Biology (2023) 64, 102759 DOI: 10.1016/j.redox.2023.102759
Weiser, Hermant, Bermont et al
Redox Biology (2023) 64, 102759 DOI: 10.1016/j.redox.2023.102759
Cited by 24
AI Summary: OriGene's MCU plasmid (#MC212635) was used to restore MCU expression in MCU-knockout HAP1 cells, enabling the authors to investigate the role of MCU in regulating mitochondrial redox state, respiration, and mobility.
Snippet: or experiments rescuing MCU expression in MCU-KO HAP1, MCU-KO cells were seeded as described above and incubated at 37 °C and 5% CO2 for 24 h. Cells were transfected with 0.75 g/well MCU plasmid (Origene, #MC212635), or with pcDNA3.1 (Thermo Fisher Scientific, #V79020, control cells) using the jetPEI DNA transfection Kit (Polyplus transfection,U.S., #101000053), carrying mitochondria-targeted
Showing 1-1 of 1 papers.
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