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Cdh8 (NM_007667) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218916
Cdh8 (Myc-DDK-tagged) - Mouse cadherin 8 (Cdh8), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $732.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cdh8
Synonyms AI851472; cad; cad8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218916 representing NM_007667
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGAAAGGCTAGCTGAGACGCTCATGGACCTCTGGACTCCATTAATAATATTATGGATTACCCTCC
CTTCTTGTGTATACACGGCTCCGATGAATCAGGCTCACGTTTTAACTACTGGATCCCCTTTGGAACTAAG
CAGGCAGAGTGAAGACATGCGGATTTTGAGCCGCTCCAAAAGAGGCTGGGTTTGGAATCAAATGTTTGTC
CTGGAAGAATTTTCTGGACCTGAACCGATTCTCGTTGGCCGGTTACACACAGATCTGGATCCTGGAAGCA
AAAAAATCAAGTATATCCTATCGGGTGATGGAGCTGGCACAATCTTTCAAATAAATGATATAACTGGAGA
TATCCATGCTATCAAAAGACTTGACCGAGAGGAAAAGGCTGAATATACGTTAACGGCTCAGGCAGTGGAC
TTTGAGACAAACAAGCCCCTTGAGCCTCCTTCTGAATTTATTATTAAGGTTCAAGATATCAACGACAATG
CACCTGAGTTCCTCAATGGACCTTATCATGCTACTGTTCCAGAGATGTCCATTTTGGGTACATCTGTCAC
TAATGTAACTGCCACTGATGCTGACGACCCAGTTTATGGAAACAGTGCAAAGTTGGTTTACAGTATCTTG
GAGGGACAGCCGTATTTTTCCATTGAGCCTGAAACAGCCATTATAAAAACTGCCCTTCCTAACATGGACA
GAGAAGCCAAGGAGGAATACCTGGTCGTAATACAAGCCAAAGATATGGGTGGACACTCTGGTGGTCTGTC
TGGAACCACAACACTCACAGTGACCCTGACTGATGTTAATGACAATCCTCCAAAATTTGCTCAGAGTTTG
TATCACTTCTCAGTACCAGAAGATGTGGTCCTTGGCACTGCGATAGGAAGGGTTAAGGCTAATGATCAGG
ATATTGGTGAAAATGCACAATCATCCTATGACATCATTGATGGAGATGGGACAGCACTATTCGAAATCAC
TTCTGATGCCCAGGCCCAGGATGGTGTTATAAGACTGAGAAAGCCTCTGGACTTTGAGACCAAAAAATCC
TATACTCTGAAGGTGGAAGCAGCCAATATCCACATCGACCCACGTTTCAGTAGCAGGGGACCCTTTAAGG
ATACAGCAACAGTCAAAATTGTCGTAGAGGATGCTGATGAGCCTCCGGTCTTCTCTTCACCAACTTACCT
CCTTGAAGTTCATGAAAATGCTGCTTTGAACTCTGTGATTGGCCAAGTGACAGCTCGTGACCCTGATATC
ACTTCCAGTCCAATAAGGTTTTCCATTGACCGCCACACTGACCTGGAAAGACAGTTCAACATCAATGCAG
ATGATGGGAAGATAACACTGGCAACTCCACTGGACAGAGAACTAAGTGTTTGGCACAACATCACCATCAT
TGCTACTGAGATCAGGAACCACAGTCAGATATCTCGAGTGCCTGTTGCTATTAAAGTGCTGGATGTCAAT
GACAACGCCCCTGAATTCGCATCCGAATATGAGGCATTTTTATGTGAAAATGGAAAACCCGGCCAAGTCA
TTCAAACAGTAAGTGCCATGGACAAAGACGATCCCAAAAACGGACATTTTTTCTTGTACAGTCTTCTTCC
AGAAATGGTCAACAACCCAAATTTCACCATCAAGAAAAATGAAGATAATTCCCTGAGCATTCTGGCAAAA
CATAATGGATTCAACCGCCAGAAGCAAGAAGTCTACCTTCTGCCTATAGTGATCAGTGACAGTGGAAACC
CCCCTCTGAGTAGCACCAGTACGTTGACCATCCGAGTCTGTGGCTGTAGCAATGATGGCGTGGTCCAGTC
GTGCAATGTTGAAGCTTATGTCCTTCCTATTGGACTCAGTATGGGCGCCTTAATTGCTATATTAGCATGC
ATCATTTTGCTGCTCGTCATTGTGGTTCTGTTTGTTACCCTGAGGAGGCACAAAAATGAACCACTTATTA
TCAAAGATGACGAAGACGTTCGAGAAAACATCATTCGCTACGACGACGAAGGAGGAGGGGAGGAGGACAC
AGAGGCTTTTGACATTGCAACTTTGCAAAACCCAGATGGAATTAATGGATTTTTACCCCGTAAGGATATT
AAACCAGATTTGCAGTTTATGCCAAGGCAAGGGCTTGCTCCAGTTCCAAATGGTGTTGATGTCGATGAAT
TTATAAATGTAAGGCTGCATGAGGCAGATAATGACCCCACGGCCCCACCATATGACTCCATACAGATTTA
TGGCTATGAAGGCCGAGGGTCTGTGGCTGGCTCCCTCAGCTCCTTGGAGTCCACCACATCAGACTCAGAC
CAGAATTTTGACTACCTCAGTGACTGGGGTCCCCGCTTTAAGAGACTGGGCGAACTCTACTCTGTTGGTG
AAAGTGACAAAGAAACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218916 representing NM_007667
Red=Cloning site Green=Tags(s)

MPERLAETLMDLWTPLIILWITLPSCVYTAPMNQAHVLTTGSPLELSRQSEDMRILSRSKRGWVWNQMFV
LEEFSGPEPILVGRLHTDLDPGSKKIKYILSGDGAGTIFQINDITGDIHAIKRLDREEKAEYTLTAQAVD
FETNKPLEPPSEFIIKVQDINDNAPEFLNGPYHATVPEMSILGTSVTNVTATDADDPVYGNSAKLVYSIL
EGQPYFSIEPETAIIKTALPNMDREAKEEYLVVIQAKDMGGHSGGLSGTTTLTVTLTDVNDNPPKFAQSL
YHFSVPEDVVLGTAIGRVKANDQDIGENAQSSYDIIDGDGTALFEITSDAQAQDGVIRLRKPLDFETKKS
YTLKVEAANIHIDPRFSSRGPFKDTATVKIVVEDADEPPVFSSPTYLLEVHENAALNSVIGQVTARDPDI
TSSPIRFSIDRHTDLERQFNINADDGKITLATPLDRELSVWHNITIIATEIRNHSQISRVPVAIKVLDVN
DNAPEFASEYEAFLCENGKPGQVIQTVSAMDKDDPKNGHFFLYSLLPEMVNNPNFTIKKNEDNSLSILAK
HNGFNRQKQEVYLLPIVISDSGNPPLSSTSTLTIRVCGCSNDGVVQSCNVEAYVLPIGLSMGALIAILAC
IILLLVIVVLFVTLRRHKNEPLIIKDDEDVRENIIRYDDEGGGEEDTEAFDIATLQNPDGINGFLPRKDI
KPDLQFMPRQGLAPVPNGVDVDEFINVRLHEADNDPTAPPYDSIQIYGYEGRGSVAGSLSSLESTTSDSD
QNFDYLSDWGPRFKRLGELYSVGESDKET

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007667
ORF Size 2397 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007667.3, NP_031693.2
RefSeq Size 3158 bp
RefSeq ORF 2400 bp
Locus ID 12564
UniProt ID P97291
Cytogenetics 8 49.4 cM
MW 88.7 kDa
Summary This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. Mice lacking the encoded protein exhibit reduced behavioral responses to cold, but not thermal stimuli. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 8. provided by RefSeq, Oct 2015
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Documents
Resources
"NM_007667" in other vectors (4)
SKU Description Size Price
MC221686 Cdh8 (untagged) - Mouse cadherin 8 (Cdh8), transcript variant 2, (10ug) 10 ug
$806.00
MG218916 Cdh8 (tGFP-tagged) - Mouse cadherin 8 (Cdh8) transcript variant 2, (10ug) 10 ug
$932.00
MR218916L3 Lenti ORF clone of Cdh8 (Myc-DDK-tagged) - Mouse cadherin 8 (Cdh8), transcript variant 2 10 ug
$1,032.00
MR218916L4 Lenti ORF clone of Cdh8 (mGFP-tagged) - Mouse cadherin 8 (Cdh8), transcript variant 2 10 ug
$1,032.00

Plasmid Map for MR218916

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218916
Circular map for MR218916

file-alt Citations

Regulation of Neural Circuit Development by Cadherin-11 Provides Implications for Autism
Frei, Niescier, Bridi et al
eNeuro (2021) 8 (4) DOI: 10.1523/ENEURO.0066-21.2021
AI Summary: OriGene contributed to this study by providing the Myc-flag-tagged full-length Cdh8 plasmid (plasmid #MR218916), which was used to investigate the role of cadherin-8 in neural circuit development related to autism.
Snippet: Myc-flag-tagged full-length Cdh8 was purchased from Origene (plasmid #MR218916). Cdh8 was expressed under the CMV promoter in the pCMV6 vector. …
FigureFigureFigure
Altered Expression of Cadherin-8 and Cadherin-11 in Neural Circuit Development: Implications for Autism
Frei, Niescier, Bridi et al
bioRxiv (2020)
Cited by 4
Snippet: Supplementary Figure 1). 103 104 Plasmids 105 Myc-flag-tagged full-length Cdh8 was purchased from Origene (plasmid #MR218916). Cdh8 was 106 expressed under the CMV promoter in the pCMV6 vector. Flag-tagged full-length
Showing 1-2 of 2 papers.
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