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Dnajc25 (NM_001033165) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218773
Dnajc25 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 25 (Dnajc25)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dnajc25
Synonyms 2010109C08Rik; 2010203O07Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218773 representing NM_001033165
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCGGCTCGCTTTGCGCGGGGGACCCGGGGCTGCCGGCCAGCGCCCATGGCTGCTGCTGGCGC
CGCTGCTGCTGGTCCCGCTGCTGGCGCGGCCCGCCGAGGCCCTGGTGGAAGGGCTGTACTGCGGCACGCG
CGACTGCTACGAGGTGCTGGGCGTGAGCCGCTCGGCCAGCAAGGCGGAGATCGCGCGGGCCTACCGTCAG
CTGGCCCGGCGCTACCACCCAGATCGCTACCGGCCCGAGCCCGGGGACGGCCCCGGGGGCGCGCCGCCGA
GCGCCGAGGCTTTCCTGCTAGTGGCGACCGCCTACGAGACGCTCAAGGATGAAGAAACACGGAAGGATTA
TGACTACATGCTGGATCACCCAGAAGAATACTATAGCCATTACTACCACTACTACAGCAGGCGCCTGGCC
CCAAAAGTGGACGTTAGGGTGGTGATTTTGGTCAGTGTGTGTGCCATTTCAATGTTTCAGTATTTCAGCT
GGTGGAATAGCTACAATAAAGCAATCAGCTACCTAGCCACAGTGCCCAAGTACCGCATCCAGGCAACAGA
AATCGCCAAGGAGCAGGGACTGCTCAAAAAAGCCAAAGAAAAAGGGAAAAACAAGAAGTCCAAAGAAGAA
ATCCGTGATGAGGAGGAGAACATCATAAAGAACATTATAAAAAGTAAAATAGACATAAAGGGGGGCTACC
AGAAACCCCAAGTTCGGGACCTCCTCTTGTTTCAGGTCATCCTCGCCCCTGTTCACCTGTGCTCTTATAT
AGCCTGGTATTGTAGGTGGATCTATAACTTTAACATCAAAGGCAAAGAGTACGGGGAGGAAGAGAGACTT
TACATTATCCGGAAGTCTATGAAGATGTCCCAGTCTCAGTTTGACAGCCTGGAGGACCATCAGAAAGAAA
TGTTTCTCAAGCGGGAGCTTTGGATAAAGGAGAATTATGAGGTCTACAAGCAGGAACAAGAGGAAGAATT
AAAGAAAAAATTGGCAAATGACCCTCGGTGGAAGCGCTACCGCAGGTGGATGAAGAATGAAGGGCCTGGA
CGGCTGACCTTTGTGGACGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218773 representing NM_001033165
Red=Cloning site Green=Tags(s)

MAARLALRGGPGAAGQRPWLLLAPLLLVPLLARPAEALVEGLYCGTRDCYEVLGVSRSASKAEIARAYRQ
LARRYHPDRYRPEPGDGPGGAPPSAEAFLLVATAYETLKDEETRKDYDYMLDHPEEYYSHYYHYYSRRLA
PKVDVRVVILVSVCAISMFQYFSWWNSYNKAISYLATVPKYRIQATEIAKEQGLLKKAKEKGKNKKSKEE
IRDEEENIIKNIIKSKIDIKGGYQKPQVRDLLLFQVILAPVHLCSYIAWYCRWIYNFNIKGKEYGEEERL
YIIRKSMKMSQSQFDSLEDHQKEMFLKRELWIKENYEVYKQEQEEELKKKLANDPRWKRYRRWMKNEGPG
RLTFVDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033165
ORF Size 1071 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033165.3, NP_001028337.2
RefSeq Size 1827 bp
RefSeq ORF 1074 bp
Locus ID 72429
UniProt ID A2ALW5
Cytogenetics 4 B3
MW 42.4 kDa
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Documents
Resources
"NM_001033165" in other vectors (4)
SKU Description Size Price
MC211329 Dnajc25 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 25 (Dnajc25), (10ug) 10 ug
$503.00
MG218773 Dnajc25 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog subfamily C member 25 (Dnajc25), (10ug) 10 ug
$657.00
MR218773L3 Lenti ORF clone of Dnajc25 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 25 (Dnajc25) 10 ug
$757.00
MR218773L4 Lenti ORF clone of Dnajc25 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 25 (Dnajc25) 10 ug
$757.00

Plasmid Map for MR218773

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218773
Circular map for MR218773

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