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Syde1 (NM_027875) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218744
Syde1 (Myc-DDK-tagged) - Mouse synapse defective 1, Rho GTPase, homolog 1 (C. elegans) (Syde1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $675.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Syde1
Synonyms 1200008N06Rik; BB043000; mSYD1A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218744 representing NM_027875
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGCCGCTGCTCAGGAAGACCTTCTCCCGCCTGCGGGGTCGTGAGAAACTTCCTCGAAAAAAGT
CAGAAGCCAAGGACCGCGGCCACCCAGCCCAGCGCTCAGAGCCCAAACCCCCAGAACCAGAACCTCGTGT
CCTTGAAGGGTCTCAGGCTGGAGCAGAGGTACCACCCAGCCCCGAGACACCTCGAAGCCCCACTCGGGGG
GCCTACCTGCAAAGCCTGGAGCCCAGTAGCCGCCGATGGGTGCTAGGAGGGGCTAAACCACCAGAGGAGA
TCTCTTTGGGGCCTAGGACACCTAGCAGTGGGGAGCCTGCTGGTGAGATTTGGTACAACCCCATCCCTGA
AGAAGACCCCAGACCACCCGCACCTGAGCCCTTGGGATCACAGCTAGCTTCCTCTGAGCCAGAAGGCCCC
AACATCCAAGGTGCAGCCCCTACCAGCCCCCCTACCAAAACCTCCCGTACCAAGTCCCCAGGCCCTGCCA
GGCGCCTCTCAATGAAGATGAAAAAGCTGCCAGAGCTCCGGCGCCGCCTAAGCCTAAGAAGTACTCGCAC
AGGCCGGGATAGAGAGAGGACCGCCCCAGCAGGCTCTGTCATCAGCCGCTATCACCTGGACAGCAGTGTG
GGGACTCCTGGGCAGGCATCAGTGGCTGGAGGGTCTAGGAGCCCAAGGGGTGGGTACCTCAGCGATGGTG
ATTCGCCTGAGCGTCCTGGAGGACCACCATCACCCACTGCCTTCCGGCCCTATGAAGTGGGTCCATCGGC
CCGGACTCCTCCAGCTGCCCTCTGGGGGCGTCTCAGCCTGCACCTGTATGGGCTAGGGGGTCTGCGACCA
AGTCCCGGGGCTACTCCACGAGATCTCTGCTGCCTTCTCCAAGTGGATGGGGTAGCTCGGGCCCGCACAG
GGCCACTTCGAAGCGGACCAGACTTCCTGAGGCTGGACCACACCTTCCACCTAGAGCTGGAGGCTGCTCG
TCTGCTGCGGGCCTTGGTACTTGCATGGGACCCTGGTGTGAGGCGGCACCGGCCCTGTGCCCAGGGCACT
GTGCTATTGCCAACCATCTTTCGAGGGTGCCAAGCGCAACAACTGGCCGTTCGACTTGAACCCCAGGGAC
TTCTTTACGCCAAGCTAACATTGTCGGAACAGCAAGAAGCCCCTGCCACAGCAGAGCCCCGTGTTTTCGG
GCTCCCTCTGCAGCTTCTGGTAGAACGTGAGCAGTCCCCAGGCCAGGTGCCTCTCATCATCCGAAAGTGT
GTGGGGCAGATCGAATGCCGAGGGCTGCGGGTAGTGGGGCTGTACCGTCTGTGTGGCTCAGCAGCAGTGA
AGAAAGAGCTTCGAGATGCCTTTGAGCAGGACAGTGCGGCTGTGTGCCTCTCTGAAGATGTGTACCCTGA
TATCAATGTCATCACAGGCATCCTCAAGGATTATCTTCGAGAACTGCCTACTCCACTCATCACCCAGCCC
CTCTACCAGGTGGTGCTGGAAGCCATGGCCCAAGGACACCCAAGCAGGGCTTCCCTGGGTCCTGAGGGCA
CCAGAGGGCTCCTCAGATGCCTGCCAGATGTGGAGAGGGCCACCCTGACGCTTCTTCTGGACCATCTGCG
CCTTGTCTCTTCCTTCCACACCCACAACCGCATGACCCCGCAGAACTTGGCAGTGTGCTTCGGACCAGTG
TTGCTACCAGCGAGACAAACACCCTCTCGGCCCCGGCTCCGTAGCTCTGGCCCAGGAGTCACCAGTGCTG
TGGACTTCAAGCGCCACATTGAAGTCCTGCATTACCTGTTACAGTCTTGGCCAGATACCAGACGGCCCTC
AGATACCCCAGATGGCGCAGTCGCACCGTATTTGAGGCCCAAAAGACAGCCACCTCTGCACTTGCCGCTG
GCGGGCCCAGAAGTGGTGACTCGGCCTCGGGGCCGCGGAGGCCCCGAAAGTCCCCCAAGCAACCGCTATG
CAGGCGACTGGAGTGTGTGCGGAGGCGATTTACTGCCCTGTGGGCGGGACTTCCTGTCCGGGCCTGACTA
TGACCATGTGACAGGCAGCGACAGTGAGGAAGATGATGATGAGACTGGAGAGCCGAGGGGCACTACCGAC
TTCGAAGATGAGTTTGATGCACCCTTCAACCCACACCTGAATCTCAAAGACTTTGATGCCCTCATCCTGG
ATCTGGAGAGAGAACTCTCCAAGCAGATCAATGTGTGCCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218744 representing NM_027875
Red=Cloning site Green=Tags(s)

MAEPLLRKTFSRLRGREKLPRKKSEAKDRGHPAQRSEPKPPEPEPRVLEGSQAGAEVPPSPETPRSPTRG
AYLQSLEPSSRRWVLGGAKPPEEISLGPRTPSSGEPAGEIWYNPIPEEDPRPPAPEPLGSQLASSEPEGP
NIQGAAPTSPPTKTSRTKSPGPARRLSMKMKKLPELRRRLSLRSTRTGRDRERTAPAGSVISRYHLDSSV
GTPGQASVAGGSRSPRGGYLSDGDSPERPGGPPSPTAFRPYEVGPSARTPPAALWGRLSLHLYGLGGLRP
SPGATPRDLCCLLQVDGVARARTGPLRSGPDFLRLDHTFHLELEAARLLRALVLAWDPGVRRHRPCAQGT
VLLPTIFRGCQAQQLAVRLEPQGLLYAKLTLSEQQEAPATAEPRVFGLPLQLLVEREQSPGQVPLIIRKC
VGQIECRGLRVVGLYRLCGSAAVKKELRDAFEQDSAAVCLSEDVYPDINVITGILKDYLRELPTPLITQP
LYQVVLEAMAQGHPSRASLGPEGTRGLLRCLPDVERATLTLLLDHLRLVSSFHTHNRMTPQNLAVCFGPV
LLPARQTPSRPRLRSSGPGVTSAVDFKRHIEVLHYLLQSWPDTRRPSDTPDGAVAPYLRPKRQPPLHLPL
AGPEVVTRPRGRGGPESPPSNRYAGDWSVCGGDLLPCGRDFLSGPDYDHVTGSDSEEDDDETGEPRGTTD
FEDEFDAPFNPHLNLKDFDALILDLERELSKQINVCL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027875
ORF Size 2211 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027875.1, NP_082151.1
RefSeq Size 3288 bp
RefSeq ORF 2214 bp
Locus ID 71709
Cytogenetics 10 C1
MW 80.9 kDa
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"NM_027875" in other vectors (4)
SKU Description Size Price
MC221240 Syde1 (untagged) - Mouse synapse defective 1, Rho GTPase, homolog 1 (C. elegans) (Syde1), (10ug) 10 ug
$743.00
MG218744 Syde1 (tGFP-tagged) - Mouse synapse defective 1 Rho GTPase homolog 1 (C. elegans) (Syde1), (10ug) 10 ug
$875.00
MR218744L3 Lenti ORF clone of Syde1 (Myc-DDK-tagged) - Mouse synapse defective 1, Rho GTPase, homolog 1 (C. elegans) (Syde1) 10 ug
$975.00
MR218744L4 Lenti ORF clone of Syde1 (mGFP-tagged) - Mouse synapse defective 1, Rho GTPase, homolog 1 (C. elegans) (Syde1) 10 ug
$975.00

Plasmid Map for MR218744

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218744
Circular map for MR218744

file-alt Citations

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