Cybrd1 (NM_028593) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR218658
Cybrd1 (Myc-DDK-tagged) - Mouse cytochrome b reductase 1 (Cybrd1)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cybrd1
Synonyms 2210407P13Rik; Dcytb
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218658 representing NM_028593
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCATGGAGGGCTACCGGGGCTTCCTGGGGCTGCTGGTGTCCGCGCTGCTCGTGGGCTTCCTGTCGG
TGATCTTCGTGCTCATCTGGGTCCTGCACTTCCGAGAGGGGCTTGGCTGGAACGGCAGCGGGCTCGAGTT
TAACTGGCACCCGGTGCTCGCGGTGACCGGCTTCGTCTTCATCCAGGGCATCGCCATCATCGTGTACAGA
CTGCCATGGACCTGGAAGTGCAGCAAGCTCTTGATGAAATCCATCCATGCCGGGTTAAACGCTGTGGCTG
CCATCCTCGCCATCATCTCCGTGGTGGCTGTGTTTGAGTACCACAATGTCCAAAAAGTCCCCCACATGTA
CAGCCTGCACAGCTGGGTTGGACTGACAGCTCTCATACTCTACATCCAACAGCTTGTTGTAGGCTTTTTC
GTCTTTCTGCTTCCATGGGCTCCGCCCTCTCTCCGGGCAATCGTCATGCCCATACACGTGTATTCTGGAC
TCCTCCTCTTCGGAACAGTGATTGCGACGGTTCTCATGGGAGTGACAGAAAAGCTGTTCTTTGTCCTGAA
ACACCCCTCGTACCATTCATTCCCACCAGAAGGTGTTTTTACAAATACGCTGGGCCTTCTGATTCTGGTG
TTTGGAGCCCTCATCTTTTGGATTGTCACCAGGCCCCAATGGAAACGCCCTAGAGAACCAGGCTCTGTCC
CTCTCCAGCTAAATGGAGGCAATGCAGAATGCAGAATGGAAGGCGCCATAGCAATAAGCTCCGCCCACAG
TATGGACGCAGCTGACCCAGCTGACGCAGAGTCAAGCAGTGAGGGAGCAGCCCGGAAGAGAACCCTCGGC
CTTGCTGACTCTGGCCAGAGGTCCACCATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR218658 representing NM_028593
Red=Cloning site Green=Tags(s)

MAMEGYRGFLGLLVSALLVGFLSVIFVLIWVLHFREGLGWNGSGLEFNWHPVLAVTGFVFIQGIAIIVYR
LPWTWKCSKLLMKSIHAGLNAVAAILAIISVVAVFEYHNVQKVPHMYSLHSWVGLTALILYIQQLVVGFF
VFLLPWAPPSLRAIVMPIHVYSGLLLFGTVIATVLMGVTEKLFFVLKHPSYHSFPPEGVFTNTLGLLILV
FGALIFWIVTRPQWKRPREPGSVPLQLNGGNAECRMEGAIAISSAHSMDAADPADAESSSEGAARKRTLG
LADSGQRSTM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028593
ORF Size 870 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028593.2, NP_082869.2
RefSeq Size 5208 bp
RefSeq ORF 873 bp
Locus ID 73649
UniProt ID Q925G2
Cytogenetics 2 C2
MW 32.3 kDa
Summary Ferric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028593" in other vectors (4)
SKU Description Size Price
MC211443 Cybrd1 (untagged) - Mouse cytochrome b reductase 1 (Cybrd1), (10ug) 10 ug
$330.00
MG218658 Cybrd1 (tGFP-tagged) - Mouse cytochrome b reductase 1 (Cybrd1), (10ug) 10 ug
$500.00
MR218658L3 Lenti ORF clone of Cybrd1 (Myc-DDK-tagged) - Mouse cytochrome b reductase 1 (Cybrd1) 10 ug
$600.00
MR218658L4 Lenti ORF clone of Cybrd1 (mGFP-tagged) - Mouse cytochrome b reductase 1 (Cybrd1) 10 ug
$600.00

Plasmid Map for MR218658

Download sequence for

Cloning scheme for MR218658

Circular map for MR218658

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