Gen1 (NM_177331) Mouse Tagged ORF Clone
$589.00
$831.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Gen1 |
| Synonyms | 5830483C08Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_177331 |
| ORF Size | 2724 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_177331.5 |
| RefSeq Size | 3302 bp |
| RefSeq ORF | 2727 bp |
| Locus ID | 209334 |
| UniProt ID | Q8BMI4 |
| Cytogenetics | 12 A1.1 |
| MW | 102.2 kDa |
| Summary | Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction. Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA. Has also endonuclease activity on 5'-flap and replication fork (RF) DNA substrates.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_177331" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC222548 | Gen1 (untagged) - Mouse Gen homolog 1, endonuclease (Drosophila) (Gen1), (10ug) | 10 ug |
$915.00
|
|
| MG218623 | Gen1 (tGFP-tagged) - Mouse Gen homolog 1 endonuclease (Drosophila) (Gen1), (10ug) | 10 ug |
$1,031.00
|
|
| MR218623L3 | Lenti ORF clone of Gen1 (Myc-DDK-tagged) - Mouse Gen homolog 1, endonuclease (Drosophila) (Gen1) | 10 ug |
$1,131.00
|
|
| MR218623L4 | Lenti ORF clone of Gen1 (mGFP-tagged) - Mouse Gen homolog 1, endonuclease (Drosophila) (Gen1) | 10 ug |
$1,131.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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