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Ndst4 (NM_022565) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218546
Ndst4 (Myc-DDK-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $798.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ndst4
Synonyms 4930439H17Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218546 representing NM_022565
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCTTATTTTAAAGTTTCGAAGAAGCTTTCGAACACTGATTGTTCTCTTAGCTACTTTTTGCTTGG
TGAGCATCCTCATTTCTGCCTACTTCCTGTACTCTGGCTACAAACAGGAAATGACACTTATTGAAACGAC
TGCAGAAGCAGAGTGTGCTGACATCAAAGACCTCCCTTATCGGTCTATAGAGCTGAGAACCATCAAGCCT
ATTGATACATCCAAAACTGACCCCACTGTCCTTCTCTTTGTAGAGAGCCAGTATTCGCAACTTGGTCAAG
ATATTATAGCAATCTTGGAGTCCAGTCGGTTTCAATACCAGATGGTCATTGCTCCAGGCAAGGGAGACAT
ACCGCCTCTTACCGATAGTGGCAAAGGGAAGTACACATTGATTATTTATGAAAATATTCTGAAGTACGTC
AGCATGGACTCATGGAACCGGGAGCTTTTAGAAAAATACTGTATAGAATACAGTGTTAGCATAATTGGTT
TTCATAAAGCCAATGAGAACAGTTTACCAACTACACAACTGAAAGGGTTTCCTTTGAACTTATTCAATAA
CGTAGCTCTGAAGGACTGCTCTGTGAACCCCCAGTCTCCTCTGCTCCATATTACCAAAGGCCCCAAAGTT
GAAAAGGGACCTCTTCCTGGGGAAGACTGGACAATTTTCCAGTATAACCATTCTACCTATCAGCCAGTGC
TTTTAACTGAGTTACAGTCAGAAAAGTCCCTCTCTTTCTTGTCCAGCCAAACACTTTATGCGACAATTAT
TCAGGATCTGGGGCTTCATGACGGGATTCAGCGAGTTCTTTTTGGTAACAACTTGAACTTTTGGTTGCAC
AAGCTCATTTTCATAGACGCCATCTCCTTCTTGTCGGGGAAGAGGTTGACACTGTCCTTGGACAGGTACA
TCCTTGTGGACATTGATGACATATTTGTGGGGAAGGAGGGCACGAGAATGAATGTCAAAGATGTGAAGGC
ATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAACCTTGGATTTTCAGGG
AAGTTTTACCACACAGGAACCGAAGAAGAGGATGAAGGTGACGACCTTCTGCTGAGGTCTGTGGATGAAT
TCTGGTGGTTTCCTCACATGTGGAGTCACATGCAGCCCCACCTCTTCCACAATGAATCATCTTTAGTGGA
ACAGATGATTCTCAACAAAGAATTTGCCCTGGAACATGGAATTCCGATCAACCTGGGCTACGCAGTGGCC
CCACATCACTCAGGGGTCTACCCAGTCCATATTCAGTTGTATGCAGCTTGGAAGAAAGTCTGGGGTATTC
AGGTCACCAGCACTGAAGAGTACCCACATCTGAAACCTGCGCGGTACAGGAAGGGCTTCATTCATAATAG
CATCATGGTCCTCCCTCGACAGACCTGTGGGCTGTTCACACATACTATTTTCTACAAGGAGTATCCAGGA
GGACCTCAAGAATTGGATAAAAGTATCAAAGGAGGTGAACTCTTCCTCACAATCCTTCTAAATCCAATCA
GCATTTTCATGACACATCTTTCTAACTATGGAAATGACCGCCTCGGCTTGTACACCTTTGTGAACTTGGC
CAACTTTGTGCACAGCTGGACCAACTTGAAACTGCAGACCCTGCCTCCAGTGCAACTGGCCCACAAATAC
TTCGAGCTCTTCCCTGAGCAGAAAGACCCTCTCTGGCAGAATCCATGCGACGATAAACGACACAAAGACA
TCTGGTCCAGGGAGAAAACCTGTGACCATTTACCGAAATTCCTTGTGATTGGGCCACAGAAGACAGGAAC
AACTGCACTTTATTTATTTCTTCTAATGCACCCCTCCATCATCAGCAATCTTCCTAGCCCCAAAACCTTT
GAAGAAGTTCAATTTTTTAATGGCAACAACTATCACAAGGGAATTGAGTGGTATATGGACTTTTTCCCCA
CTCCTTCCAACATTACCAGTGATTTCCTATTTGAAAAGAGTGCTAACTACTTCCACTCAGAAGAGGCTCC
CAAGAGGGCTGCATCTCTTGTTCCCAAAGCCAAGATCATTACTATCCTCATAGACCCCTCGGACAGGGCA
TATTCTTGGTACCAGCACCAACGATCTCATGAAGATCCAGCTGCCCTGAGGTTCAATTTCTATGAAGTTA
TCACAACAGGACATTGGGCCCCTCCTGATTTAAAAACATTGCAGAGGAGATGTCTGGTGCCTGGGTGGTA
TGCAGTGCACATAGAAAGATGGCTGGCCTACTTTTCTACTTCCCAGTTGCTAATTATTGATGGACAACAG
CTGAGATCTGACCCAGCTACTGTGATGGATGAAGTCCAGAAGTTTCTAGGAGTTACACCTCATTATAATT
ACTCGGAAGCACTAACGTTTGATCCCCAGAAAGGCTTTTGGTGTCAGCTACTAGAAGGAGGAAAAACCAA
ATGCCTTGGGAAAAGCAAAGGCCGAAAATACCCGCCCATGGATTCAGAGTCCAGAACTTTCCTCTCCAGC
TACTACCGCGACCACAACGTGGAACTCTCGAAGCTGCTGCACAGGCTGGGGCAGCCTCTGCCCTCGTGGC
TGAGACAGGAGCTGCAGAAAGTGAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218546 representing NM_022565
Red=Cloning site Green=Tags(s)

MNLILKFRRSFRTLIVLLATFCLVSILISAYFLYSGYKQEMTLIETTAEAECADIKDLPYRSIELRTIKP
IDTSKTDPTVLLFVESQYSQLGQDIIAILESSRFQYQMVIAPGKGDIPPLTDSGKGKYTLIIYENILKYV
SMDSWNRELLEKYCIEYSVSIIGFHKANENSLPTTQLKGFPLNLFNNVALKDCSVNPQSPLLHITKGPKV
EKGPLPGEDWTIFQYNHSTYQPVLLTELQSEKSLSFLSSQTLYATIIQDLGLHDGIQRVLFGNNLNFWLH
KLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSG
KFYHTGTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINLGYAVA
PHHSGVYPVHIQLYAAWKKVWGIQVTSTEEYPHLKPARYRKGFIHNSIMVLPRQTCGLFTHTIFYKEYPG
GPQELDKSIKGGELFLTILLNPISIFMTHLSNYGNDRLGLYTFVNLANFVHSWTNLKLQTLPPVQLAHKY
FELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIISNLPSPKTF
EEVQFFNGNNYHKGIEWYMDFFPTPSNITSDFLFEKSANYFHSEEAPKRAASLVPKAKIITILIDPSDRA
YSWYQHQRSHEDPAALRFNFYEVITTGHWAPPDLKTLQRRCLVPGWYAVHIERWLAYFSTSQLLIIDGQQ
LRSDPATVMDEVQKFLGVTPHYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDSESRTFLSS
YYRDHNVELSKLLHRLGQPLPSWLRQELQKVR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022565
ORF Size 2616 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022565.2, NP_072087.2
RefSeq Size 3489 bp
RefSeq ORF 2619 bp
Locus ID 64580
UniProt ID Q9EQW8
Cytogenetics 3 G1
MW 101.1 kDa
Summary Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_022565" in other vectors (4)
SKU Description Size Price
MC222328 Ndst4 (untagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4), (10ug) 10 ug
$799.00
MG218546 Ndst4 (tGFP-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4), (10ug) 10 ug
$998.00
MR218546L3 Lenti ORF clone of Ndst4 (Myc-DDK-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4) 10 ug
$1,098.00
MR218546L4 Lenti ORF clone of Ndst4 (mGFP-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4) 10 ug
$1,098.00

Plasmid Map for MR218546

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218546
Circular map for MR218546

file-alt Citations

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