Gsap (NM_175437) Mouse Tagged ORF Clone

SKU
MR218533
Pion (Myc-DDK-tagged) - Mouse pigeon homolog (Drosophila) (Pion)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

    Click here to learn more.

$589.00 MSRP $786.00 MSRP $786.00
In Stock*
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gsap
Synonyms A530088I07Rik; Pion
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218533 representing NM_175437
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTGCGGCTGGTCACTCACTTCGATGTGCTGGAGGACGTGCTCCCGAGTCTGCTCACACAGGCGG
CGACAACGGACGAAGGAGACAGAGCAGGTGTTTTAGAAACAACTTATGGGAGCTTACGTGTACTAAACAT
CGAAAGAAATGGAAACATTATTTATACCTATAAGGACAATAAGGGAAACGCTGTTTTTGGATTATATGAT
TGCCAAACCAGACAGAATGAGCATCTGTACACCTTTGAGAAGGACATGCAAGCTGTCAGTTGTTCAGTCA
ACAGTGAGAGAACAGTGCTTGCTGCGAGTTTCATTCAGTATACCACAGAGGGAGTAAAGAATGACCTTCA
GCCAGGATCGAAGTGCCTAACTTTGCTGGTAGAAATACACCCTGTTAACAACGTGAAGGTTCTGAAAGCA
GTGGATAGCTGTGTTTGGGTACAGTTCCTCTACCCACAAGCTGAAAGTCATCTTCTTCCACAGAACCACC
TGCTACTGATCTCAGAAGAGAAATATATTGAGCGGTTTCATATCCAGATCACCAGAGAAGATGGAGACAG
AGTGGTGATTAGAAATTCTAGCCATCTCCCAAGAGACAGACTAGCTGAAGACTTTGTTTGGGCTCAGTGG
GATCTGTCAGAGCAGAGACTATACTACATTGAACTAAAGGAGTCAAGGAGTATCTTGAAATGCATCCAGT
TTAGGGCAGATGAAAGTTTTAACTTAATGTTTGAAATGCCCTTGGACATAACGTTAACTGGCTTGAGATT
TAAACTTGTCAACTTTGGATATGACTATCGTCAAGACCGTGAGAAGCTGTGCAATCAGCCTTCTCTCTGC
ATTTTTACCAACCATACAGGGAGTTTGTGTATGTGTTACAGCCCTAAGTCTGACTCACGGGAAGAAATCA
CATATTCAGTGTTCTACCTCCATAAAGGATACAGAAAGATCTTCACTGCAGCTCCTGGAAGTGCTGACTC
ACAGGTCACAAACGGTGCTGACTCACAGGTCACAGACGGCATCGCTTTCCTCAACCTTGGCTACTTTGTG
GCTGTTTACTCCCCTGGCCATTTCCTTCATTTACTTAACATTCAGCATCCAGACTTGGTCTGCCACAGTC
TGTTTCTGACAGGAAACAACAAAATAGCTGCTGTGCTACCTCCAAGTCCTTTGCAGTCCCTGCCGGGGTC
CCTGGTTCTGGACTGCTATTCTGGAAAGGTCTATAGAGTAACCCTTGACCAGTCATACTTGTTGCGGTTC
CTATGGAACGCCCATCTCGACTGTGAGAGAATGGCTGCACTGCACTGTATACTATCCTGTAGTCAAGACC
CAGGTTTCCCGGAAGAACAGATTATCCAGTGGATTTCGGAGCATGTCTCTGCTTGCCATTCATTTGACCT
CATTCAAGAATTTCTAATTGCTTCCTCCTATTGGAGTGTGTATGCAGAGCTGGACGACATGGGCATGCTC
CTACAATACTCCTCTGTGCTGACTTGGAACACAGAAATTCCCGGAATAAAGTTTACGACAGAGGAACTCC
CATTGCCTCTTATGAAGGTCTACGGCTTGAAGGGGTACTGGGCAAAGCTGAACTCCAACCTTGAGTACAT
TAAGTATACAAAACCTCACTTGCACTATCACAACAGCGTCGTGAGGAGGGAATGGCATAACCTGATCTCT
GAAGAGAGAACGGGCAAAAGGAGATCCACAATGTACGTGAGGAACATTCTTGAAAACGCAATGAAGGTGA
TTGCTAGCATGGAAACGAGGACACTGGAGCCAAGATTAATACCATTCTTACAAGAGGAAGACCGGCACCA
GAGGCTGCTCATGGGCTTGATGGTGTCTGAGCTAAGAGACCACCTACTGAGACACCTGCAGGGTGTAGAA
AAGAAGAAAATTGAACAGATGGTTCTGGACTACATTTCAAAACTGCTGGATCTCATTTGGTGCTTGCTGG
AAACCAGTTGGAGGAAACACAGTATGCATCCATTGGTTCTCCACCTCAATAGCCATTGCAGTGCTGCAGA
TTTTGAAGTCTTTCATCTCATGACCAGGATTTTGGATGCTGCTAGCAGTTTGTGTTTGCCTCTGCCTCCT
GGTTTCCACTCTCTGCACACGATCCTTGGGGTCCACTGCCTCCCTTTGTACAGCCTTCTGCACTACATTG
ATAACGGAGTGCTGCTGCTTACTGAGACCGCGGTCACGAGGCTTATGAAAGATCTGGACAATTCAGAGAA
AAATGAACAACTGAAATTCAGCATCATTGTCCGGCTCCCTCCGCTTATTGGCCAGAAGGTCTGCCGGCTT
TGGGACCACCCCATGAGTTCCAATATCATTTCACGGAACCATGTGGCCCGGCTGCTGAAAAACTACAGGA
AAGAGCCTCGGAACTCCATGATTGACAAATCATCATTTCCGGTAGAATTTCTGCCTCTGAACTACTTCAT
CGAAATTCTGATGGGCCTGGAGTCCTCCAACCAAGCACTGTATGGCTTTGAAGGACATGACAATGTGGAC
GCAGAATTTGTAGAGGAAGCTGCTCTGAAACACACCACCATGCTTTTGGGCTTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR218533 representing NM_175437
Red=Cloning site Green=Tags(s)

MALRLVTHFDVLEDVLPSLLTQAATTDEGDRAGVLETTYGSLRVLNIERNGNIIYTYKDNKGNAVFGLYD
CQTRQNEHLYTFEKDMQAVSCSVNSERTVLAASFIQYTTEGVKNDLQPGSKCLTLLVEIHPVNNVKVLKA
VDSCVWVQFLYPQAESHLLPQNHLLLISEEKYIERFHIQITREDGDRVVIRNSSHLPRDRLAEDFVWAQW
DLSEQRLYYIELKESRSILKCIQFRADESFNLMFEMPLDITLTGLRFKLVNFGYDYRQDREKLCNQPSLC
IFTNHTGSLCMCYSPKSDSREEITYSVFYLHKGYRKIFTAAPGSADSQVTNGADSQVTDGIAFLNLGYFV
AVYSPGHFLHLLNIQHPDLVCHSLFLTGNNKIAAVLPPSPLQSLPGSLVLDCYSGKVYRVTLDQSYLLRF
LWNAHLDCERMAALHCILSCSQDPGFPEEQIIQWISEHVSACHSFDLIQEFLIASSYWSVYAELDDMGML
LQYSSVLTWNTEIPGIKFTTEELPLPLMKVYGLKGYWAKLNSNLEYIKYTKPHLHYHNSVVRREWHNLIS
EERTGKRRSTMYVRNILENAMKVIASMETRTLEPRLIPFLQEEDRHQRLLMGLMVSELRDHLLRHLQGVE
KKKIEQMVLDYISKLLDLIWCLLETSWRKHSMHPLVLHLNSHCSAADFEVFHLMTRILDAASSLCLPLPP
GFHSLHTILGVHCLPLYSLLHYIDNGVLLLTETAVTRLMKDLDNSEKNEQLKFSIIVRLPPLIGQKVCRL
WDHPMSSNIISRNHVARLLKNYRKEPRNSMIDKSSFPVEFLPLNYFIEILMGLESSNQALYGFEGHDNVD
AEFVEEAALKHTTMLLGL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_175437
ORF Size 2574 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175437.3, NP_780646.2
RefSeq Size 3745 bp
RefSeq ORF 2577 bp
Locus ID 212167
UniProt ID Q3TCV3
Cytogenetics 5 A3
MW 99.1 kDa
Summary Regulator of gamma-secretase activity, which specifically activates the production of amyloid-beta protein (amyloid-beta protein 40 and amyloid-beta protein 42), without affecting the cleavage of other gamma-secretase targets such has Notch. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Specifically promotes the gamma-cleavage of APP CTF-alpha (also named APP-CTF) by the gamma-secretase complex to generate amyloid-beta, while it reduces the epsilon-cleavage of APP CTF-alpha, leading to a low production of AICD.[UniProtKB/Swiss-Prot Function]
Write Your Own Review
You're reviewing:Gsap (NM_175437) Mouse Tagged ORF Clone
Your Rating
SKU Description Size Price
MC222180 Gsap (untagged) - Mouse pigeon homolog (Drosophila) (Pion), (10ug) 10 ug
$865.00
MG218533 Pion (tGFP-tagged) - Mouse pigeon homolog (Drosophila) (Pion), (10ug) 10 ug
$986.00
MR218533L3 Lenti ORF clone of Pion (Myc-DDK-tagged) - Mouse pigeon homolog (Drosophila) (Pion) 10 ug
$1,086.00
MR218533L4 Lenti ORF clone of Pion (mGFP-tagged) - Mouse pigeon homolog (Drosophila) (Pion) 10 ug
$1,086.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.