Gsap (NM_175437) Mouse Untagged Clone

SKU MC222180

Gsap (untagged) - Mouse pigeon homolog (Drosophila) (Pion), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Gsap
• Synonyms: A530088I07Rik; Pion
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222180 representing NM_175437; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTCTGCGGCTGGTCACTCACTTCGATGTGCTGGAGGACGTGCTCCCGAGTCTGCTCACACAGGCGG; CGACAACGGACGAAGGAGACAGAGCAGGTGTTTTAGAAACAACTTATGGGAGCTTACGTGTACTAAACAT; CGAAAGAAATGGAAACATTATTTATACCTATAAGGACAATAAGGGAAACGCTGTTTTTGGATTATATGAT; TGCCAAACCAGACAGAATGAGCATCTGTACACCTTTGAGAAGGACATGCAAGCTGTCAGTTGTTCAGTCA; ACAGTGAGAGAACAGTGCTTGCTGCGAGTTTCATTCAGTATACCACAGAGGGAGTAAAGAATGACCTTCA; GCCAGGATCGAAGTGCCTAACTTTGCTGGTAGAAATACACCCTGTTAACAACGTGAAGGTTCTGAAAGCA; GTGGATAGCTGTGTTTGGGTACAGTTCCTCTACCCACAAGCTGAAAGTCATCTTCTTCCACAGAACCACC; TGCTACTGATCTCAGAAGAGAAATATATTGAGCGGTTTCATATCCAGATCACCAGAGAAGATGGAGACAG; AGTGGTGATTAGAAATTCTAGCCATCTCCCAAGAGACAGACTAGCTGAAGACTTTGTTTGGGCTCAGTGG; GATCTGTCAGAGCAGAGACTATACTACATTGAACTAAAGGAGTCAAGGAGTATCTTGAAATGCATCCAGT; TTAGGGCAGATGAAAGTTTTAACTTAATGTTTGAAATGCCCTTGGACATAACGTTAACTGGCTTGAGATT; TAAACTTGTCAACTTTGGATATGACTATCGTCAAGACCGTGAGAAGCTGTGCAATCAGCCTTCTCTCTGC; ATTTTTACCAACCATACAGGGAGTTTGTGTATGTGTTACAGCCCTAAGTCTGACTCACGGGAAGAAATCA; CATATTCAGTGTTCTACCTCCATAAAGGATACAGAAAGATCTTCACTGCAGCTCCTGGAAGTGCTGACTC; ACAGGTCACAAACGGTGCTGACTCACAGGTCACAGACGGCATCGCTTTCCTCAACCTTGGCTACTTTGTG; GCTGTTTACTCCCCTGGCCATTTCCTTCATTTACTTAACATTCAGCATCCAGACTTGGTCTGCCACAGTC; TGTTTCTGACAGGAAACAACAAAATAGCTGCTGTGCTACCTCCAAGTCCTTTGCAGTCCCTGCCGGGGTC; CCTGGTTCTGGACTGCTATTCTGGAAAGGTCTATAGAGTAACCCTTGACCAGTCATACTTGTTGCGGTTC; CTATGGAACGCCCATCTCGACTGTGAGAGAATGGCTGCACTGCACTGTATACTATCCTGTAGTCAAGACC; CAGGTTTCCCGGAAGAACAGATTATCCAGTGGATTTCGGAGCATGTCTCTGCTTGCCATTCATTTGACCT; CATTCAAGAATTTCTAATTGCTTCCTCCTATTGGAGTGTGTATGCAGAGCTGGACGACATGGGCATGCTC; CTACAATACTCCTCTGTGCTGACTTGGAACACAGAAATTCCCGGAATAAAGTTTACGACAGAGGAACTCC; CATTGCCTCTTATGAAGGTCTACGGCTTGAAGGGGTACTGGGCAAAGCTGAACTCCAACCTTGAGTACAT; TAAGTATACAAAACCTCACTTGCACTATCACAACAGCGTCGTGAGGAGGGAATGGCATAACCTGATCTCT; GAAGAGAGAACGGGCAAAAGGAGATCCACAATGTACGTGAGGAACATTCTTGAAAACGCAATGAAGGTGA; TTGCTAGCATGGAAACGAGGACACTGGAGCCAAGATTAATACCATTCTTACAAGAGGAAGACCGGCACCA; GAGGCTGCTCATGGGCTTGATGGTGTCTGAGCTAAGAGACCACCTACTGAGACACCTGCAGGGTGTAGAA; AAGAAGAAAATTGAACAGATGGTTCTGGACTACATTTCAAAACTGCTGGATCTCATTTGGTGCTTGCTGG; AAACCAGTTGGAGGAAACACAGTATGCATCCATTGGTTCTCCACCTCAATAGCCATTGCAGTGCTGCAGA; TTTTGAAGTCTTTCATCTCATGACCAGGATTTTGGATGCTGCTAGCAGTTTGTGTTTGCCTCTGCCTCCT; GGTTTCCACTCTCTGCACACGATCCTTGGGGTCCACTGCCTCCCTTTGTACAGCCTTCTGCACTACATTG; ATAACGGAGTGCTGCTGCTTACTGAGACCGCGGTCACGAGGCTTATGAAAGATCTGGACAATTCAGAGAA; AAATGAACAACTGAAATTCAGCATCATTGTCCGGCTCCCTCCGCTTATTGGCCAGAAGGTCTGCCGGCTT; TGGGACCACCCCATGAGTTCCAATATCATTTCACGGAACCATGTGGCCCGGCTGCTGAAAAACTACAGGA; AAGAGCCTCGGAACTCCATGATTGACAAATCATCATTTCCGGTAGAATTTCTGCCTCTGAACTACTTCAT; CGAAATTCTGATGGGCCTGGAGTCCTCCAACCAAGCACTGTATGGCTTTGAAGGACATGACAATGTGGAC; GCAGAATTTGTAGAGGAAGCTGCTCTGAAACACACCACCATGCTTTTGGGCTTATGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175437
• Insert Size: 2577 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_175437.3, NP_780646.2
• RefSeq Size: 3745 bp
• RefSeq ORF: 2577 bp
• Locus ID: 212167
• UniProt ID: Q3TCV3
• Cytogenetics: 5 A3
• Summary: Regulator of gamma-secretase activity, which specifically activates the production of amyloid-beta protein (amyloid-beta protein 40 and amyloid-beta protein 42), without affecting the cleavage of other gamma-secretase targets such has Notch. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Specifically promotes the gamma-cleavage of APP CTF-alpha (also named APP-CTF) by the gamma-secretase complex to generate amyloid-beta, while it reduces the epsilon-cleavage of APP CTF-alpha, leading to a low production of AICD.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).