Stac (NM_016853) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR218513
Stac (Myc-DDK-tagged) - Mouse src homology three (SH3) and cysteine rich domain (Stac)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Stac
Synonyms MGC130287; MGC130288
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218513 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTCCTCCAAGTGGCGCCCGCGAGGACAGCGGAGACGGACTGACCGGGGAGGCAACGGGCACAGAGC
AGCCGCCCTCTCCTGCGTCCACCAGCAGCCTGGAATCCAAGCTCCAGAAACTAAAACGGTCACTTTCTTT
CAAGACCAAGAGCTTGCGGAGCAAAAGTGCTGACAACTTCTTCCCAAGAACCAACAGTGATGTGAAACCG
CAAGCAGACCTGCTGGCCAAGGCCAGCCCAGGCCCCAGCCCAATAGCCATCCCTGGAAGCCCAGCGTCCA
TGCCCACCAAGGCTGGCCTGCACCCCGGCAGCAACAGCAAGCTCCACGCCTTTCAGGAGCATGTCTTTAA
GAAGCCCACCTTCTGTGATGTCTGCAACCACATGATCGTGGGAACACATGCTAAGCACGGACTGCGCTGC
GGAGCCTGTAAGATGAGCATCCACCACAAGTGTGCAGATGGCCTGGCGCCCCAGCGGTGCATGGGCAAGT
TGCCAAAGGGATTTCGGCGTTACTACAGCTCCCCCTTGCTCATTCACGAACAGTTTGGATGCATTAAAGA
AGTTATGCCCATTGCGTGTGGCAATAAAGTGGACCCCGTGTATGAGGCCCTCCGGTTCGGCACGTCCCTG
GCCCAGAGAACGAAGAAGGGCGGCTCAGGCAGTGGTTCTGACTCACCGCCCAGAACCTCGACTTCAGAGC
TTGTAGACGTCCCTGAAGAGGCCGATGGGCCAGGAGATGGATCTGACATGAGGACACGGAGCAACAGTGT
GTTTACATATCCAGAAAACGGTATGGATGACTTCAGAGATCAAATGAAGACCACAAACCACCAGGGACCT
CTTTCCAAAGACCCATTACAGATGAACACCTACGTTGCCTTGTACAGATTTATACCACAGGAGAATGAAG
ACTTGGAAATGAGGCCAGGAGACATGATCACTCTCCTAGAAGACTCCAATGAAGACTGGTGGAAAGGAAA
GATTCAGGACAGGGTTGGTTTCTTTCCAGCCAACTTTGTTCAGAGAGTAGAAGAGCATGAGAAGATTTAT
AGGTGTGTTCGAACCTTCATTGGCTGCAAGGACCAGGGGCAGATAACACTGAAAGAGAACCAGATATGCG
TGACCTCTGAGGAAGAACAGGACGGCTTCATCAGAGTCCTCAGTGGGAAGAAGAGAGGCCTCGTCCCACT
GGATGTACTGGTAGACGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR218513 protein sequence
Red=Cloning site Green=Tags(s)

MIPPSGAREDSGDGLTGEATGTEQPPSPASTSSLESKLQKLKRSLSFKTKSLRSKSADNFFPRTNSDVKP
QADLLAKASPGPSPIAIPGSPASMPTKAGLHPGSNSKLHAFQEHVFKKPTFCDVCNHMIVGTHAKHGLRC
GACKMSIHHKCADGLAPQRCMGKLPKGFRRYYSSPLLIHEQFGCIKEVMPIACGNKVDPVYEALRFGTSL
AQRTKKGGSGSGSDSPPRTSTSELVDVPEEADGPGDGSDMRTRSNSVFTYPENGMDDFRDQMKTTNHQGP
LSKDPLQMNTYVALYRFIPQENEDLEMRPGDMITLLEDSNEDWWKGKIQDRVGFFPANFVQRVEEHEKIY
RCVRTFIGCKDQGQITLKENQICVTSEEEQDGFIRVLSGKKRGLVPLDVLVDV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016853
ORF Size 1209 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016853.3
RefSeq Size 2515 bp
RefSeq ORF 1212 bp
Locus ID 20840
UniProt ID P97306
Cytogenetics 9 61.81 cM
MW 44.3 kDa
Summary Promotes expression of the ion channel CACNA1H at the cell membrane, and thereby contributes to the regulation of channel activity (PubMed:27149520). Plays a minor and redundant role in promoting the expression of calcium channel CACNA1S at the cell membrane, and thereby contributes to increased channel activity (PubMed:29467163). Slows the rate of calcium-mediated inactivation of CACNA1C calcium channel activity (PubMed:29363593).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016853" in other vectors (4)
SKU Description Size Price
MC209469 Stac (untagged) - Mouse src homology three (SH3) and cysteine rich domain (Stac), (10ug) 10 ug
$686.00
MG218513 Stac (tGFP-tagged) - Mouse src homology three (SH3) and cysteine rich domain (Stac), (10ug) 10 ug
$886.00
MR218513L3 Lenti ORF clone of Stac (Myc-DDK-tagged) - Mouse src homology three (SH3) and cysteine rich domain (Stac) 10 ug
$986.00
MR218513L4 Lenti ORF clone of Stac (mGFP-tagged) - Mouse src homology three (SH3) and cysteine rich domain (Stac) 10 ug
$986.00

Plasmid Map for MR218513

Download sequence for

Cloning scheme for MR218513

Circular map for MR218513

file-alt Citations

STAC2 negatively regulates osteoclast formation by targeting the RANK signaling complex
Jeong, Choi, Park et al
Cell Death Differ. (2018) 25 (8), 1364-1374 DOI: 10.1038/s41418-017-0048-5
AI Summary: OriGene products pCMV6-Entry-mSTAC1 and pCMV6-Entry-mSTAC3 expression vectors were used to study the roles of STAC family proteins in the regulation of RANK-mediated osteoclast formation in this paper.
Snippet: Article.;he pCMV6-Entry- mSTAC1 and pCMV6-Entry-mSTAC3 vectors were pur- chased from OriGene Technologies. The retroviral vector containing a constitutively active form of NFATc1 (CaN- FATc1) has been previously described
Showing 1-1 of 1 papers.
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