Ranbp3 (NM_027933) Mouse Tagged ORF Clone
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Ranbp3 |
| Synonyms | 2610024N24Rik; AA408221 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_027933 |
| ORF Size | 1473 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_027933.3, NP_082209.1 |
| RefSeq Size | 2511 bp |
| RefSeq ORF | 1476 bp |
| Locus ID | 71810 |
| UniProt ID | Q9CT10 |
| Cytogenetics | 17 D |
| MW | 52.6 kDa |
| Summary | Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_027933" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC216846 | Ranbp3 (untagged) - Mouse RAN binding protein 3 (Ranbp3), (10ug) | 10 ug |
$503.00
|
|
| MG218464 | Ranbp3 (tGFP-tagged) - Mouse RAN binding protein 3 (Ranbp3), (10ug) | 10 ug |
$657.00
|
|
| MR218464L3 | Lenti ORF clone of Ranbp3 (Myc-DDK-tagged) - Mouse RAN binding protein 3 (Ranbp3) | 10 ug |
$757.00
|
|
| MR218464L4 | Lenti ORF clone of Ranbp3 (mGFP-tagged) - Mouse RAN binding protein 3 (Ranbp3) | 10 ug |
$757.00
|
|
Citations
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