Phb2 (NM_007531) Mouse Tagged ORF Clone
$289.00
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Phb2 |
| Synonyms | AU044498; BAP; Bap37; Bcap37; REA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007531 |
| ORF Size | 897 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007531.2, NP_031557.2 |
| RefSeq Size | 1360 bp |
| RefSeq ORF | 900 bp |
| Locus ID | 12034 |
| UniProt ID | O35129 |
| Cytogenetics | 6 59.17 cM |
| MW | 33.3 kDa |
| Summary | Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. In mitochondria, regulates cytochrome-c oxidase assembly (COX) and mitochondrial respiration. Binding to sphingoid 1-phosphate (SPP) modulates its regulator activity (PubMed:20959514).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007531" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC208177 | Phb2 (untagged) - Mouse prohibitin 2 (Phb2), (10ug) | 10 ug |
$330.00
|
|
| MG218376 | Phb2 (tGFP-tagged) - Mouse prohibitin 2 (Phb2), (10ug) | 10 ug |
$489.00
$500.00
|
|
| MR218376L3 | Lenti ORF clone of Phb2 (Myc-DDK-tagged) - Mouse prohibitin 2 (Phb2) | 10 ug |
$600.00
|
|
| MR218376L4 | Lenti ORF clone of Phb2 (mGFP-tagged) - Mouse prohibitin 2 (Phb2) | 10 ug |
$600.00
|
|
Citations
Apolipoproteins L1 and L3 Control Mitochondrial Membrane Dynamics
Lecordier, Heo, Graversen et al
Cell Rep (2023) 42 (12), 113528 DOI: 10.1016/j.celrep.2023.113528
Lecordier, Heo, Graversen et al
Cell Rep (2023) 42 (12), 113528 DOI: 10.1016/j.celrep.2023.113528
Cited by 26
AI Summary: OriGene products contributed to this study by providing recombinant PHB2 protein (Cat. TP760501) and the PHB2 plasmid (Cat. MR218376), which were used to investigate the interactions of apolipoproteins L1 and L3 with mitophagy-related proteins and their role in mitochondrial membrane dynamics.
Snippet: zureau et al.11 N/A Reccombinant NCS1 L89K Uzureau et al.11 N/A Recombinant Calneuron-1 Novus Biologicals NBP1-50858 Recombinant Calneuron-1 Prospec pro-279 Recombinant PHB2 Origene TP760501 Recombinant VAMP8 Abnova 8673-P01 Recombinant VAMP8 Novus Biologicals NBC1-18348 Recombinant VAMP8 Heo et al.132 N/A Recombinant sVAMP8 Heo et al.132 N/A;- pro-279);PHB2 (Origene Cat. TP760501);VAMP8 (Abnova Cat. 8673-P01 and PHB2 was amplified from Origene plasmid Cat. MR218376. …
Inner mitochondrial membrane protein Prohibitin 1 mediates Nix-induced, Parkin-independent mitophagy
Alula, Delgado-Deida, Callahan et al
Sci Rep (2023) 13 (1), 18 DOI: 10.1038/s41598-022-26775-x
Alula, Delgado-Deida, Callahan et al
Sci Rep (2023) 13 (1), 18 DOI: 10.1038/s41598-022-26775-x
Cited by 27
AI Summary: OriGene’s Mouse PHB2 overexpression plasmid (MR218376) was used in this study to investigate the role of Prohibitin 1 in mediating Nix-induced, Parkin-independent mitophagy.
Snippet: Solution GS5245, Qiagen, referred to as siPhb1b herein), or Stealth RNAi siRNA Negative Control Med GC (Invitrogen) at 20 µm concentration for 48 h. Mouse PHB2 overexpression plasmid (MR218376, Origene), pSELECT-GFP-LC3 expression plasmid (#psetz-gfplc3, Invivogen), mito-RFP-EGFP expression plasmid31, or pGL3-Nix65 (a generous gift from Dr. Gerald Dorn, Washington University, St. Louis) were
Prohibitin levels regulate OMA1 activity and turnover in neurons
Anderson, Kahl, Fruitman et al
Cell Death Differ. (2019)
Anderson, Kahl, Fruitman et al
Cell Death Differ. (2019)
Cited by 64
Cytoplasmic sequestration of the RhoA effector mDiaphanous1 by Prohibitin2 promotes muscle differentiation
Saleh, Subramaniam, Raychaudhuri et al
Sci Rep (2019) 9 (1), 8302 DOI: 10.1038/s41598-019-44749-4
Saleh, Subramaniam, Raychaudhuri et al
Sci Rep (2019) 9 (1), 8302 DOI: 10.1038/s41598-019-44749-4
AI Summary: OriGene contributed to this study by providing the Flag-tagged mouse Phb2 full-length (Phb2 FL) expression plasmid, which was used to investigate the functional interaction between Prohibitin2 and mDia1 during muscle differentiation.
Snippet: … mDia1ΔN3-BD was generated by directional cloning in pGBKT7 (GAL4 binding domain vector-Clonetech) whereas Flag-tagged mouse Phb2 expression plasmids Phb2-Y2H (89–299aa), Phb2-Amino (89–180aa), Phb2-Central (140–244 aa), Phb2-Carboxy (180–299 aa) and Phb2 120–232 (120–232 aa) were generated using directional cloning in pCMV2B. Flag-tagged mouse Phb2 FL was obtained from Origene, MyoG prom-pGL3 was a gift from Eric Olson’s lab64, TCF reporters Super 8X TOP-flash (TCF site)/FOP-flash (mutated TCF site)97, and 3DA.luc30 were gifts from R.T. …
Showing 1-4 of 4 papers.
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