Phb2 (NM_007531) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG218376
Phb2 (tGFP-tagged) - Mouse prohibitin 2 (Phb2), (10ug)
$489.00 $500.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Phb2
Synonyms AU044498; BAP; Bap37; Bcap37; REA
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG218376 representing NM_007531
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAGAACTTGAAGGACTTAGCTGGACGCCTGCCCGCCGGGCCTCGGGGCATGGGCACGGCGCTGA
AGCTGCTGCTGGGGGCCGGGGCGGTGGCCTACGGCGTCCGCGAATCCGTGTTCACCGTGGAAGGCGGTCA
TAGAGCCATCTTTTTTAATCGTATTGGTGGCGTGCAGCAGGACACGATCCTGGCCGAAGGCCTTCACTTC
AGGATCCCCTGGTTCCAGTACCCCATCATCTATGACATTCGGGCCAGACCTCGAAAAATCTCCTCCCCCA
CAGGCTCCAAAGACCTGCAGATGGTGAACATCTCCCTGCGTGTGCTGTCCCGACCCAATGCCCAGGAGCT
CCCCAGCATGTACCAGCGTCTAGGGCTGGACTATGAGGAGCGAGTGCTGCCGTCCATTGTTAATGAGGTG
CTCAAGAGTGTGGTGGCCAAGTTCAATGCCTCGCAGCTGATCACCCAGCGGGCTCAGGTGTCCCTGTTGA
TCCGAAGAGAGCTGACAGAGCGCGCCAAGGACTTCAGCCTCATCCTGGATGATGTAGCTATCACAGAGCT
GAGCTTCAGCCGAGAGTACACAGCTGCTGTAGAAGCCAAGCAAGTGGCCCAGCAGGAAGCCCAGCGGGCC
CAGTTTTTGGTGGAGAAAGCGAAGCAGGAACAGCGACAGAAGATTGTGCAGGCTGAGGGGGAGGCGGAGG
CTGCCAAGATGCTTGGAGAAGCACTGAGCAAGAATCCTGGCTATATCAAGCTCCGAAAGATCCGGGCCGC
CCAGAACATCTCTAAAACGATCGCCACATCACAGAACCGAATCTATCTCACAGCTGACAACCTTGTGCTG
AATCTACAGGATGAAAGTTTTACTCGGGGAAGTGACAGCCTCATTAAGGGTAAGAAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG218376 representing NM_007531
Red=Cloning site Green=Tags(s)

MAQNLKDLAGRLPAGPRGMGTALKLLLGAGAVAYGVRESVFTVEGGHRAIFFNRIGGVQQDTILAEGLHF
RIPWFQYPIIYDIRARPRKISSPTGSKDLQMVNISLRVLSRPNAQELPSMYQRLGLDYEERVLPSIVNEV
LKSVVAKFNASQLITQRAQVSLLIRRELTERAKDFSLILDDVAITELSFSREYTAAVEAKQVAQQEAQRA
QFLVEKAKQEQRQKIVQAEGEAEAAKMLGEALSKNPGYIKLRKIRAAQNISKTIATSQNRIYLTADNLVL
NLQDESFTRGSDSLIKGKK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007531
ORF Size 897 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007531.2, NP_031557.2
RefSeq Size 1360 bp
RefSeq ORF 900 bp
Locus ID 12034
UniProt ID O35129
Cytogenetics 6 59.17 cM
Summary Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. In mitochondria, regulates cytochrome-c oxidase assembly (COX) and mitochondrial respiration. Binding to sphingoid 1-phosphate (SPP) modulates its regulator activity (PubMed:20959514).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007531" in other vectors (4)
SKU Description Size Price
MC208177 Phb2 (untagged) - Mouse prohibitin 2 (Phb2), (10ug) 10 ug
$330.00
MR218376 Phb2 (Myc-DDK-tagged) - Mouse prohibitin 2 (Phb2) 10 ug
$289.00 $300.00
MR218376L3 Lenti ORF clone of Phb2 (Myc-DDK-tagged) - Mouse prohibitin 2 (Phb2) 10 ug
$600.00
MR218376L4 Lenti ORF clone of Phb2 (mGFP-tagged) - Mouse prohibitin 2 (Phb2) 10 ug
$600.00

Plasmid Map for MG218376

Download sequence for

Cloning scheme for MG218376

Circular map for MG218376

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