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Dusp13 (NM_013849) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218320
Dusp13 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 13 (Dusp13), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dusp13
Synonyms DUSP13A; DUSP13B; Gm1203; LMW-D; LMW-DSP6; MDSP; TMD; TMDP; TS-D; TS-DSP6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218320 representing NM_013849
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGCTGGGCAGAGACCACTGCAGTCTGCCCTTGGCATTTACCTGCCAAAGTCTCTTTCCCAGACAC
CTCGATGTCCTTCTGTGAGAACGCTAGCCCCACTTGGTCTGTGCTCCCTAAGAGAAGAGGGGAGACAGAG
AGGGAACAGCAGAGGAGACCAGGAGAAATGTGTTCTGAGGTTACAGCTAAAGAGAATGGACTCGCTACAG
AAGCAGGAACTTCGGAGGCCAAAGATTCATGGGGCAGTCCAGGTGTCCCCCTACCAGCCACCCACACTGG
CCTCTCTGCAGCGATTGCTGTGGGTCCGTCGGACTGCCACACTGACCCACATCAATGAGGTCTGGCCCAA
CCTTTTCTTGGGAGATGCGTATGCTGCCAGAGACAAGGGTCGTCTAATCCAGCTGGGCATTACCCATGTT
GTGAATGTGGCTGCGGGCAAGTTCCAGGTGGACACAGGTGCCAAGTTCTACCGTGGAACACCTCTGGAGT
ACTATGGCATTGAGGCTGATGACAACCCCTTCTTTGACCTCAGCGTCCACTTTCTGCCTGTTGCTCGTTA
CATCAGAGATGCCCTCAATATTCCCCGAAGCCGAGTGCTGGTCCACTGCGCTATGGGGGTGAGTCGCTCT
GCCACAATTGTCTTGGCCTTCCTCATGATCTTCGAGAACATGACACTGGTAGATGCCATCCAGACGGTGC
AGGCCCACCGAGATATCTGTCCCAACTCAGGCTTCCTCCGACAGCTCCAGGTTCTGGACAACAGGCTGAG
GCGGGAAACAGGAAGACTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218320 representing NM_013849
Red=Cloning site Green=Tags(s)

MQAGQRPLQSALGIYLPKSLSQTPRCPSVRTLAPLGLCSLREEGRQRGNSRGDQEKCVLRLQLKRMDSLQ
KQELRRPKIHGAVQVSPYQPPTLASLQRLLWVRRTATLTHINEVWPNLFLGDAYAARDKGRLIQLGITHV
VNVAAGKFQVDTGAKFYRGTPLEYYGIEADDNPFFDLSVHFLPVARYIRDALNIPRSRVLVHCAMGVSRS
ATIVLAFLMIFENMTLVDAIQTVQAHRDICPNSGFLRQLQVLDNRLRRETGRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013849
ORF Size 789 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013849.3, NP_038877.2
RefSeq Size 1071 bp
RefSeq ORF 792 bp
Locus ID 27389
UniProt ID Q9QYJ7
Cytogenetics 14 A3
MW 29.7 kDa
Summary Members of the protein-tyrosine phosphatase superfamily cooperate with protein kinases to regulate cell proliferation and differentiation. This superfamily is separated into two families based on the substrate that is dephosphorylated. One family, the dual specificity phosphatases (DSPs) acts on both phosphotyrosine and phosphoserine/threonine residues. This gene encodes different but related DSP proteins through the use of non-overlapping open reading frames, alternate splicing, and presumed different transcription promoters. Expression of the distinct proteins from this gene has been found to be tissue specific and the proteins may be involved in postnatal development of specific tissues. A protein encoded by the upstream ORF was found in skeletal muscle, whereas the encoded protein from the downstream ORF was found only in testis. In humans, a similar pattern of expression was found. Multiple alternatively spliced transcript variants were described, but the full-length sequence of only some were determined. provided by RefSeq, Jul 2008
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"NM_013849" in other vectors (4)
SKU Description Size Price
MC209789 Dusp13 (untagged) - Mouse dual specificity phosphatase 13 (Dusp13), transcript variant 2, (10ug) 10 ug
$330.00
MG218320 Dusp13 (tGFP-tagged) - Mouse dual specificity phosphatase 13 (Dusp13) transcript variant 2, (10ug) 10 ug
$500.00
MR218320L3 Lenti ORF clone of Dusp13 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 13 (Dusp13), transcript variant 2 10 ug
$600.00
MR218320L4 Lenti ORF clone of Dusp13 (mGFP-tagged) - Mouse dual specificity phosphatase 13 (Dusp13), transcript variant 2 10 ug
$600.00

Plasmid Map for MR218320

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218320
Circular map for MR218320

file-alt Citations

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