Pate4 (NM_020264) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR218297
Pate4 (Myc-DDK-tagged) - Mouse prostate and testis expressed 4 (Pate4)
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pate4
Synonyms 9530004K16Rik; Pate-B; Svs7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218297 representing NM_020264
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTCAGTGACGAAAATCAGCACACTGCTCATCGTGATTTTATCCTTTCTCTGTTTTGTGGAGGGTC
TGATCTGTAATTCATGTGAAAAGTCACGAGATTCAAGATGTACAATGTCACAAAGCAGATGCGTTGCAAA
ACCTGGTGAATCATGCAGTACCGTATCACACTTTGTTGGAACAAAGCATGTGTACTCAAAGCAAATGTGC
TCGCCTCAGTGCAAGGAAAAACAGCTTAACACTGGAAAGAAGTTGATTTACATAATGTGCTGCGAAAAAA
ACTTGTGTAATAGCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR218297 representing NM_020264
Red=Cloning site Green=Tags(s)

MNSVTKISTLLIVILSFLCFVEGLICNSCEKSRDSRCTMSQSRCVAKPGESCSTVSHFVGTKHVYSKQMC
SPQCKEKQLNTGKKLIYIMCCEKNLCNSF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020264
ORF Size 297 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020264.4, NP_064660.2
RefSeq Size 1016 bp
RefSeq ORF 300 bp
Locus ID 56872
UniProt ID Q09098
Cytogenetics 9 A4
MW 11.5 kDa
Summary Enhances sperm motility. Binds to calmodulin and inhibits calcium transport into spermatozoa. May modulate the function of nicotinic acetylcholine receptors.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_020264" in other vectors (4)
SKU Description Size Price
MC210112 Pate4 (untagged) - Mouse prostate and testis expressed 4 (Pate4), (10ug) 10 ug
$165.00
MG218297 Pate4 (tGFP-tagged) - Mouse prostate and testis expressed 4 (Pate4), (10ug) 10 ug
$350.00
MR218297L3 Lenti ORF clone of Pate4 (Myc-DDK-tagged) - Mouse prostate and testis expressed 4 (Pate4) 10 ug
$450.00
MR218297L4 Lenti ORF clone of Pate4 (mGFP-tagged) - Mouse prostate and testis expressed 4 (Pate4) 10 ug
$450.00

Plasmid Map for MR218297

Download sequence for

Cloning scheme for MR218297

Circular map for MR218297

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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