Rsph3a (NM_025789) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR218229
Rsph3a (Myc-DDK-tagged) - Mouse radial spoke 3A homolog (Chlamydomonas) (Rsph3a)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rsph3a
Synonyms 1700012G05Rik; 4930524H12Rik; R74860; Rshl2; Rshl2a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218229 representing NM_025789
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGACCGTAACCCTCGGACAGCCGAGGCCTCAGGCCTCTACACCTATTCCAGCAGGCCCCGGGCCG
TGGCTTGCCAGCGCCGCCGCCACCGAGACAGCATTTTGCAGCCAGTTGAAGAACCTATGAGTTACGGGAA
CATCATGTATGACAGAAGGGTGATCCGAGGCAACACCTATGCACTGCCCACAGGACAGGTGCCTGGACAG
CCTGATCCTCTAGAGCTTCAGCGACAGCAGCAGGCCAGGAGAAGGGCTCTTGCCAGAAAACGAGCCCAGG
AGCAGCTCAAACCACGAACGCCTGAGCCCGTGGAGGGCAGAAAGCATGTGGACATACAAACAGAATTATA
CCTTGAAGAAATTGCTGATCGCATAGTTGAAGTCGACATGGAATGCCAGACAGATGCGTTTCTGGACAGA
CCACCCACACCCCTCTTCATTCCTGCCAAAACTGGCAAAGATGTGGCCACTCAGATCCTGGGAGGAGAGC
TTTTCGACTTTGACCTTGAAGTCAAGCCCATGTTAGAGGTTTTGGTGGGGAAGACGATTGAACAGTCCCT
TCTAGAGGTGATGGAAGAGGAGGAGCTGGCCAACCTGCGGGCCAGGCAGTACGCATATGAAGAGATAAGG
AACGTGGAGCTGGCCGAAGTGCAGCGACTGGAAGAGCAAGAGAGAAGGCACCGAGAAGAAAAGGAGCGCC
GTAAGAAACAGCAGTGGGAGATTGTCCATAAGCGGAATGAGACGTTACAGAAGATCTCAGCTCTGATATT
CGCTCGGCAGTACCTGGCTAACCTCCTCCCGTCTGTCTTTGACAAACTCAGGAATAGTGGCTTCTTTTAT
GATCCAATTGAAAGAGATATTGAGGTAGGATTCCTCCCATGGCTAATGAATGAAGTTGAAAAATCCATGG
AACACAGCATGGTGGGAAGAACTGTGCTTGACATGTTAATTCGCGACGTGGTTGAAAGAAGGATCAACGA
TTACGAGCACAAGGAAGCCATGCCGCCTGGTCAGAAGACAAATGTGATTAATGGTCCTAACACAGTGACA
GATCCCTCGGTGACCACATTGCACACGCAGAAGCCAGTTTTAGACAGAGTCTCCTCACAGCCAGCACCAT
CCCAAGAAAGGAAGCCTGTGGAAGAGGGGGGACACCTTATGGCAGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR218229 representing NM_025789
Red=Cloning site Green=Tags(s)

MTDRNPRTAEASGLYTYSSRPRAVACQRRRHRDSILQPVEEPMSYGNIMYDRRVIRGNTYALPTGQVPGQ
PDPLELQRQQQARRRALARKRAQEQLKPRTPEPVEGRKHVDIQTELYLEEIADRIVEVDMECQTDAFLDR
PPTPLFIPAKTGKDVATQILGGELFDFDLEVKPMLEVLVGKTIEQSLLEVMEEEELANLRARQYAYEEIR
NVELAEVQRLEEQERRHREEKERRKKQQWEIVHKRNETLQKISALIFARQYLANLLPSVFDKLRNSGFFY
DPIERDIEVGFLPWLMNEVEKSMEHSMVGRTVLDMLIRDVVERRINDYEHKEAMPPGQKTNVINGPNTVT
DPSVTTLHTQKPVLDRVSSQPAPSQERKPVEEGGHLMAE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025789
ORF Size 1167 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025789.1, NP_080065.1
RefSeq Size 1960 bp
RefSeq ORF 1551 bp
Locus ID 66832
UniProt ID Q3UFY4
Cytogenetics 17 A1
MW 45.2 kDa
Summary Functions as a protein kinase A-anchoring protein that scaffolds the cAMP-dependent protein kinase holoenzyme. May serve as a point of convergence for MAPK and PKA signaling in cilia (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025789" in other vectors (4)
SKU Description Size Price
MC217310 Rsph3a (untagged) - Mouse radial spoke 3A homolog (Chlamydomonas) (Rsph3a), (10ug) 10 ug
$529.00
MG218229 Rsph3a (tGFP-tagged) - Mouse radial spoke 3A homolog (Chlamydomonas) (Rsph3a), (10ug) 10 ug
$886.00
MR218229L3 Lenti ORF clone of Rsph3a (Myc-DDK-tagged) - Mouse radial spoke 3A homolog (Chlamydomonas) (Rsph3a) 10 ug
$986.00
MR218229L4 Lenti ORF clone of Rsph3a (mGFP-tagged) - Mouse radial spoke 3A homolog (Chlamydomonas) (Rsph3a) 10 ug
$986.00

Plasmid Map for MR218229

Download sequence for

Cloning scheme for MR218229

Circular map for MR218229

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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