Msh3 (NM_010829) Mouse Tagged Lenti ORF Clone
- LentiORF®
SKU
MR218117L3
Lenti ORF clone of Msh3 (Myc-DDK-tagged) - Mouse mutS homolog 3 (E. coli) (Msh3)
$1,253.00
3 Weeks*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Msh3 |
| Synonyms | D13Em1; Rep-3; Rep3 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_010829 |
| ORF Size | 3285 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_010829.2, NP_034959.2 |
| RefSeq Size | 3945 bp |
| RefSeq ORF | 3288 bp |
| Locus ID | 17686 |
| UniProt ID | P13705 |
| Cytogenetics | 13 47.63 cM |
| Summary | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_010829" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC223548 | Msh3 (untagged) - Mouse mutS homolog 3 (E. coli) (Msh3), (10ug) | 10 ug |
$1,049.00
|
|
| MG218117 | Msh3 (tGFP-tagged) - Mouse mutS homolog 3 (E. coli) (Msh3), (10ug) | 10 ug |
$1,153.00
|
|
| MR218117 | Msh3 (Myc-DDK-tagged) - Mouse mutS homolog 3 (E. coli) (Msh3) | 10 ug |
$589.00
$953.00
|
|
| MR218117L4 | Lenti ORF clone of Msh3 (mGFP-tagged) - Mouse mutS homolog 3 (E. coli) (Msh3) | 10 ug |
$1,253.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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