Pla2g2f (NM_012045) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR218100
Pla2g2f (Myc-DDK-tagged) - Mouse phospholipase A2, group IIF (Pla2g2f)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pla2g2f
Synonyms GIIFsPLA2; sPLA2-IIF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218100 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGATGGGGCACAGGCCAACCCCAAAGGGTTCAGGAAGAAGGCGCTGGTTAAACACTCCACTGGAC
GGAAGAGCCCAAGCCTCAGGGCCTCTCCCTCTAAAACCTCCAGGTCCAGCCTGGGTATGAAGAAATTCTT
TGCCATCGCAGTCCTGGCCGGCAGTGTGGTAACCACGGCCCACAGCAGCCTGCTGAACCTGAAGTCCATG
GTGGAGGCCATCACACACAGAAACTCCATCCTGTCCTTTGTGGGCTACGGCTGCTACTGCGGGCTGGGGG
GACGCGGCCATCCCATGGATGAGGTAGACTGGTGCTGCCATGCCCACGACTGTTGCTATGAGAAGCTCTT
TGAGCAGGGCTGCCGCCCCTACGTGGACCACTATGACCACAGGATCGAGAATGGCACCATGATTGTCTGC
ACTGAGCTCAATGAGACGGAGTGTGACAAGCAGACATGCGAGTGTGACAAGAGCCTGACTCTGTGCCTCA
AGGATCACCCATACAGGAACAAGTACCGAGGCTACTTCAACGTCTACTGCCAGGGCCCCACACCCAACTG
CAGCATCTATGACCCGTACCCAGAGGAAGTCACCTGTGGGCATGGCCTCCCTGCGACCCCTGTCTCAACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR218100 protein sequence
Red=Cloning site Green=Tags(s)

MADGAQANPKGFRKKALVKHSTGRKSPSLRASPSKTSRSSLGMKKFFAIAVLAGSVVTTAHSSLLNLKSM
VEAITHRNSILSFVGYGCYCGLGGRGHPMDEVDWCCHAHDCCYEKLFEQGCRPYVDHYDHRIENGTMIVC
TELNETECDKQTCECDKSLTLCLKDHPYRNKYRGYFNVYCQGPTPNCSIYDPYPEEVTCGHGLPATPVST

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012045
ORF Size 630 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012045.4, NP_036175.2
RefSeq Size 2437 bp
RefSeq ORF 633 bp
Locus ID 26971
UniProt ID Q9QZT4
Cytogenetics 4 D3
MW 23.3 kDa
Summary May play a role in lipid mediator production in inflammatory conditions, by providing arachidonic acid to downstream cyclooxygenases and lipoxygenases. Phospholipase A2, which catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:10531313). Hydrolyzes phosphatidylethanolamine more efficiently than phosphatidylcholine, with only a modest preference for arachidonic acid versus linoelic acid at the sn-2 position. Comparable activity toward 1-palmitoyl-2-oleoyl-phosphatidylserine vesicles to that toward 1-palmitoyl-2-oleoyl-phosphatidylglycerol (PubMed:11877435). Prefers phosphatidylglycerol compared to phosphatidylcholine (PubMed:10531313).UniProtKB/Swiss-Prot Function
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Resources
"NM_012045" in other vectors (4)
SKU Description Size Price
MC209755 Pla2g2f (untagged) - Mouse phospholipase A2, group IIF (Pla2g2f), (10ug) 10 ug
$330.00
MG218100 Pla2g2f (tGFP-tagged) - Mouse phospholipase A2 group IIF (Pla2g2f), (10ug) 10 ug
$500.00
MR218100L3 Lenti ORF clone of Pla2g2f (Myc-DDK-tagged) - Mouse phospholipase A2, group IIF (Pla2g2f) 10 ug
$600.00
MR218100L4 Lenti ORF clone of Pla2g2f (mGFP-tagged) - Mouse phospholipase A2, group IIF (Pla2g2f) 10 ug
$600.00

Plasmid Map for MR218100

Download sequence for

Cloning scheme for MR218100

Circular map for MR218100

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