Pla2g2f (NM_012045) Mouse Tagged ORF Clone
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Pla2g2f |
| Synonyms | GIIFsPLA2; sPLA2-IIF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012045 |
| ORF Size | 630 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012045.4, NP_036175.2 |
| RefSeq Size | 2437 bp |
| RefSeq ORF | 633 bp |
| Locus ID | 26971 |
| UniProt ID | Q9QZT4 |
| Cytogenetics | 4 D3 |
| MW | 23.3 kDa |
| Summary | May play a role in lipid mediator production in inflammatory conditions, by providing arachidonic acid to downstream cyclooxygenases and lipoxygenases. Phospholipase A2, which catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:10531313). Hydrolyzes phosphatidylethanolamine more efficiently than phosphatidylcholine, with only a modest preference for arachidonic acid versus linoelic acid at the sn-2 position. Comparable activity toward 1-palmitoyl-2-oleoyl-phosphatidylserine vesicles to that toward 1-palmitoyl-2-oleoyl-phosphatidylglycerol (PubMed:11877435). Prefers phosphatidylglycerol compared to phosphatidylcholine (PubMed:10531313).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012045" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC209755 | Pla2g2f (untagged) - Mouse phospholipase A2, group IIF (Pla2g2f), (10ug) | 10 ug |
$330.00
|
|
| MG218100 | Pla2g2f (tGFP-tagged) - Mouse phospholipase A2 group IIF (Pla2g2f), (10ug) | 10 ug |
$500.00
|
|
| MR218100L3 | Lenti ORF clone of Pla2g2f (Myc-DDK-tagged) - Mouse phospholipase A2, group IIF (Pla2g2f) | 10 ug |
$600.00
|
|
| MR218100L4 | Lenti ORF clone of Pla2g2f (mGFP-tagged) - Mouse phospholipase A2, group IIF (Pla2g2f) | 10 ug |
$600.00
|
|
Citations
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.