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Atrip (NM_172774) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218096
Atrip (Myc-DDK-tagged) - Mouse ATR interacting protein (Atrip)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $719.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atrip
Synonyms 6620401K05Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218096 representing NM_172774
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGGACCCCCGCACCGAACAGCCACAGGAAACAGAGTGGCGGCCTGGAGCCTTTCCCCGGTCTGT
CTCGATCCATTGAGAACCCTCCGAGCAAGCGGGCCCGGAGCTTCTCTGAGACCACAGTCCCGGACCCCGA
AGACCCATTCGGTGAGCACGCGGAATTTACTGCGGACGACCTAGAGGAACTCGACATCCTCGCGTCACAG
GCCCTGAGCCAGTGTCCCGTCGCGCCTCGGAACCTGTCCAGTGCTCATAAGGTCCGCCGATTAGATGGGT
TACCAAATAGTCCTATAAGGAAAAGCAGAGAAGATATTCCAGTTAAAGATAATTTTGAATTAGAAGTACT
TCAGATACAATACAAAGAACTTAAAGAAAAGCTGAAAGCAATGGAAGAAGAAATCCTAATTAAGAATGGA
GAAATTAAAATTTTGCGTGACTCTCTGCGTCAGACAGAATCCGTTCTAGAAGAACAGAAAAGATCACATT
TCCTTCTTGAGCAAGAAAAGACTCAAGCACTCAGTGAAAAGGAAAAGGAATTCTCCAGAAAGCTCCAATC
ACTGCAGTCTGAACTGCAGTTTAAAGACGCAGAAATGAATGAGTTAAGGACAAAGTCTCAGAGCAACGGA
CGAACAAATAAGCCAGCCGCTCCCTCCGTCTCCCACGTCAGTCCTAGGAAAGGCTCTTCTGTGGTTCTCA
AGTCAGAAGCATGTTCTCCACATGTTGGAAAAACAACTTTCCCTACAAAAGAATCTTTTAGTGCTAACAC
GCCTCTCTTCCACCCCTGTCAGACTGAGGCAGGACACAGATTTCTGGTGGGCCAAGAGGTTTCAGATAAT
AAAAACCACAGTCTGGGAGGTAGCCTCATGAAGCAAGATGTGCAGCAGAGAATTCTGGCTGATGGCTGGA
TGCAGAGAAAAGATGCTCAAGGTTCTATTTTGATAAACTTGCTCCTGAAGCAACCTTTGGTGCCAGGGTC
ATCTCTAGGTCTTTGTCACCTTCTGAGCAGTTGTCCTGAGGTTCCTACTGGCACCCTCTTGCAGCCACCA
GGGCTCAGTACTTTGCCTGGGACTTCAGGCCTCAGGACCATCAGTTCTTCTGATGGGCCATTTTCCCCCT
CTGCCCTGAGAGAAGCACAGAACTTGGCATTCACTGGATTAAATCTGGTTGCCAGGACTGAGAGCTCACA
TGATGGAGACATGGCAGGCAGAAGAGTCTTCCCGCTCCACCAGCTTCCTGGAGCTGTGCATCTCCTTCCT
CTTGTGCAGTTTTTCGTCGGCTTACACTGCCAGGCTCTGCAAGATTTAGCCCCAGCTAAGAAGAGTGGGG
TGCCTGGGGACTCTGCGACACATACCTCCTGCATGAGCTCTGGGGTCGAGGCTAGCCCAGAGGACTCCAT
TCATGGCTTGGAGAGCTTTTCTGTGGCATCACTCAGCGTCCTTCAGAACCTGGTGTGCCACAGTGGGGCA
GTTGTCTGCCTGTTACTGTCAGGCATGGGGACAGAAGCTGCCGCCAGGGAAGGAAACTTGGTCCAGACTT
GTGCAGATACAACCTCAGCCTCCAGGGAGGATGCTCATGACCAAGACCAGCACCCACTGCTAAAGATGCT
TCTTCAGCTGATGGCTTCCTCCTCTGCAGCATCAGGTCATTTTCAAGCCAGTGTCCTGGGCCTGTGCCTC
AAAGTTTTGGTGAAATTAGCAGAAAACGCTTCCTCTGATTTGTTGCCCAGGTTCTCCTGTGTGTTCCCGG
TGTTGCCACAGTGCCTCGGCTCAGCGCTGCCGCTGCCCTGTGTGCTCCTGGCTGTTGAGCTGCTCTCTGT
GCTGCTGGACCATGACAGCCTAGCATGGCAGCTATGCTCCCACCCAGAAGGCTGCCTTCTCCTGAGGCTG
TACATGTACATCACATCAAGGCCTGACAGAACGGCCTCAGAGACACAGTGGCTTCAGCTGGAACAAGAGG
TAGTATGGCTCTTGGCCAAGCTGAGTGTACAGAGCCCTGCCCCCGCTGGCATTGGTTCTGACTGTCACTG
CAATGTAGAGGCAGTCAGAGCGCTCACTGTGATGTTGCACAGGCAATGGCTGACGGTGCGGCGGGCAGGA
GGTCCAAGGACCCACCAGCAGAAGCAGACGATACGCTGTCTGCGGGACACTGTGCTGCTGCTGCATAGCC
TATCTCAGAAGGACAAGCTCTTCACTGTGCACTGTGTCGAGGTCCTGCATCAGTACGACCAGGTGATGCC
AGGGGTCAGCATGCTGATTCGAGCCCTTCCTGATGTGACTGACTGTGAAGAGGCTGCCCTGGATGACCTC
TGTGCTGCGGAGACTGATTTGGAAGACTCTGAGATGGACTGTAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218096 representing NM_172774
Red=Cloning site Green=Tags(s)

MAGTPAPNSHRKQSGGLEPFPGLSRSIENPPSKRARSFSETTVPDPEDPFGEHAEFTADDLEELDILASQ
ALSQCPVAPRNLSSAHKVRRLDGLPNSPIRKSREDIPVKDNFELEVLQIQYKELKEKLKAMEEEILIKNG
EIKILRDSLRQTESVLEEQKRSHFLLEQEKTQALSEKEKEFSRKLQSLQSELQFKDAEMNELRTKSQSNG
RTNKPAAPSVSHVSPRKGSSVVLKSEACSPHVGKTTFPTKESFSANTPLFHPCQTEAGHRFLVGQEVSDN
KNHSLGGSLMKQDVQQRILADGWMQRKDAQGSILINLLLKQPLVPGSSLGLCHLLSSCPEVPTGTLLQPP
GLSTLPGTSGLRTISSSDGPFSPSALREAQNLAFTGLNLVARTESSHDGDMAGRRVFPLHQLPGAVHLLP
LVQFFVGLHCQALQDLAPAKKSGVPGDSATHTSCMSSGVEASPEDSIHGLESFSVASLSVLQNLVCHSGA
VVCLLLSGMGTEAAAREGNLVQTCADTTSASREDAHDQDQHPLLKMLLQLMASSSAASGHFQASVLGLCL
KVLVKLAENASSDLLPRFSCVFPVLPQCLGSALPLPCVLLAVELLSVLLDHDSLAWQLCSHPEGCLLLRL
YMYITSRPDRTASETQWLQLEQEVVWLLAKLSVQSPAPAGIGSDCHCNVEAVRALTVMLHRQWLTVRRAG
GPRTHQQKQTIRCLRDTVLLLHSLSQKDKLFTVHCVEVLHQYDQVMPGVSMLIRALPDVTDCEEAALDDL
CAAETDLEDSEMDCN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172774
ORF Size 2355 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172774.3, NP_766362.2
RefSeq Size 2572 bp
RefSeq ORF 2358 bp
Locus ID 235610
UniProt ID Q8BMG1
Cytogenetics 9 F2
MW 86 kDa
Summary Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172774" in other vectors (4)
SKU Description Size Price
MC221598 Atrip (untagged) - Mouse ATR interacting protein (Atrip), (10ug) 10 ug
$792.00
MG218096 Atrip (tGFP-tagged) - Mouse ATR interacting protein (Atrip), (10ug) 10 ug
$919.00
MR218096L3 Lenti ORF clone of Atrip (Myc-DDK-tagged) - Mouse ATR interacting protein (Atrip) 10 ug
$1,019.00
MR218096L4 Lenti ORF clone of Atrip (mGFP-tagged) - Mouse ATR interacting protein (Atrip) 10 ug
$1,019.00

Plasmid Map for MR218096

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218096
Circular map for MR218096

file-alt Citations

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