Ccl1 (NM_011329) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR217886
Ccl1 (Myc-DDK-tagged) - Mouse chemokine (C-C motif) ligand 1 (Ccl1)
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccl1
Synonyms BF534335; I-309; P500; Scya1; Tca-3; Tca3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217886 representing NM_011329
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAACCCACTGCCATGGCACTGATGTGCCTGCTGCTGGCTGCCGTGTGGATACAGGATGTTGACAGCA
AGAGCATGCTTACGGTCTCCAATAGCTGCTGCTTGAACACCTTGAAGAAAGAGCTTCCCCTGAAGTTTAT
CCAGTGTTACAGAAAGATGGGCTCCTCCTGTCCTGATCCCCCAGCTGTGGTATTCAGGCTGAACAAAGGT
AGAGAAAGCTGCGCCTCAACTAACAAAACGTGGGTTCAAAATCACCTGAAGAAGGTGAACCCCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011329
ORF Size 276 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011329.3, NP_035459.1
RefSeq Size 505 bp
RefSeq ORF 279 bp
Locus ID 20290
UniProt ID P10146
Cytogenetics 11 49.99 cM
MW 10.7 kDa
Summary Cytokine that is chemotactic for neutrophils.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011329" in other vectors (4)
SKU Description Size Price
MC209361 Ccl1 (untagged) - Mouse chemokine (C-C motif) ligand 1 (Ccl1), (10ug) 10 ug
$150.00
MG217886 Ccl1 (tGFP-tagged) - Mouse chemokine (C-C motif) ligand 1 (Ccl1), (10ug) 10 ug
$350.00
MR217886L3 Lenti ORF clone of Ccl1 (Myc-DDK-tagged) - Mouse chemokine (C-C motif) ligand 1 (Ccl1) 10 ug
$450.00
MR217886L4 Lenti ORF clone of Ccl1 (mGFP-tagged) - Mouse chemokine (C-C motif) ligand 1 (Ccl1) 10 ug
$450.00

Plasmid Map for MR217886

Download sequence for

Cloning scheme for MR217886

Circular map for MR217886

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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