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Arhgef3 (NM_027871) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217816
Arhgef3 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (Arhgef3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $494.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arhgef3
Synonyms 1200004I24Rik; 9830169H03Rik; C76747; Xpln
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217816 representing NM_027871
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGTCGGAGCGCCCCATGGTGTGGTGCTGTTTTTTTCTCCGTGCACAGCGGAAACGAAAACAGAGTT
CCCAGGATGAAGATGCTGTCAGCCTGTGCAGTCTTGACATAAGTGAACCTAGTAACAAAAGGGTCAAACC
CCTTTCCAGAGTCACATCGCTAGCAAACCTCATTCCACCTGTGAAGACCACACCATTAAAGCGCTTCAGC
CAGACCTTGCAGCGGTCCATCAGCTTCCGCAGCGAGAGCCGCCCCGACATCCTTGCCCCCCGAGCTTGGT
CCAGAAATGCCACCTCCTCAAGCACAAAGCGGAGAGACAGCAAACTGTGGAGTGAGACCTTCGATGTGTG
TGTCAATCAGGTGCTCACGGCCAAGGAGATCAAGAGACAGGAGGCGATCTTTGAACTTTCTCAAGGTGAA
GAAGACTTGATAGAGGACTTGAAATTAGCAAAAAAGGCCTACCACGACCCCATGCTGAAACTCTCCATAA
TGACGGAACAGGAACTGAATCAGATTTTTGGGACCCTGGACTCTCTAATTCCTCTCCATGAAGAGCTCCT
CAGTCAGCTTCGAGATGTTCGGAAACCCGATGGCTCGACTGAGCACGTTGGTCCCATCCTCGTGGGCTGG
CTGCCCTGTCTCAGCTCCTACGACAGCTACTGCAGCAATCAAGTCGCCGCCAAAGCCCTGCTGGACCACA
AAAAGCAGGACCACAGAGTGCAGGACTTCCTCCAGCGCTGTTTAGAATCACCCTTCAGTCGCAAACTCGA
TCTCTGGAATTTCCTTGATATTCCCCGGAGCCGGCTAGTGAAGTATCCTCTGCTCCTTCGAGAAATCCTG
AGGCATACACCAAATGATAACCCAGACCAGCAGCACCTGGAAGAAGCTATAAACATCATCCAGGGAATTG
TGGCAGAAATCAACACCAAGACTGGGGAATCGGAATGCCGCTATTACAAAGAGCGGCTCCTTTACCTGGA
AGAAGGCCAGAAAGATTCTCTGATTGACAGCTCAAGGGTCCTGTGTTGTCATGGAGAGCTGAAAAATAAC
CGGGGTGTGAAACTCCATGTTTTCCTGTTCCAAGAAGTGCTGGTGATCACCCGAGCTGTCACTCACAACG
AGCAGCTCTGCTATCAGTTGTACCGACAGCCCATCCCTGTGAAGGACCTCACGCTGGAAGACCTGCAGGA
TGGAGAAGTGCGGCTGGGTGGCTCCCTGCGCGGGGCCTTCAGCAACAACGAGAGAGTTAAAAACTTCTTC
AGAGTCAGTTTCAAAAATGGATCCCAAAGTCAGACCCACTCACTACAAGCCAACGACACCTTCAACAAAC
AGCAGTGGCTCAACTGTATCCGTCAGGCAAAAGAAACTGTTCTGTCTGCTGCCGGCCAGGCTGGCTTGCT
TGACTCTGAGGGACTGGTCCAGGGTCCCGGCACTGAGAACAGGGAGCCACAGGGAGAAACAAAACTTGAG
CAGATGGACCAATCGGACAGCGAGTCGGACTGCAGTATGGACACCAGTGAGGTCAGCCTTGAGTGTGAGC
GGATGGAACAGACAGACGCTTCCTGTGCAAACAGCAGGCCCGAGGAGAGTGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217816 representing NM_027871
Red=Cloning site Green=Tags(s)

MRSERPMVWCCFFLRAQRKRKQSSQDEDAVSLCSLDISEPSNKRVKPLSRVTSLANLIPPVKTTPLKRFS
QTLQRSISFRSESRPDILAPRAWSRNATSSSTKRRDSKLWSETFDVCVNQVLTAKEIKRQEAIFELSQGE
EDLIEDLKLAKKAYHDPMLKLSIMTEQELNQIFGTLDSLIPLHEELLSQLRDVRKPDGSTEHVGPILVGW
LPCLSSYDSYCSNQVAAKALLDHKKQDHRVQDFLQRCLESPFSRKLDLWNFLDIPRSRLVKYPLLLREIL
RHTPNDNPDQQHLEEAINIIQGIVAEINTKTGESECRYYKERLLYLEEGQKDSLIDSSRVLCCHGELKNN
RGVKLHVFLFQEVLVITRAVTHNEQLCYQLYRQPIPVKDLTLEDLQDGEVRLGGSLRGAFSNNERVKNFF
RVSFKNGSQSQTHSLQANDTFNKQQWLNCIRQAKETVLSAAGQAGLLDSEGLVQGPGTENREPQGETKLE
QMDQSDSESDCSMDTSEVSLECERMEQTDASCANSRPEESV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027871
ORF Size 1593 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027871.2, NP_082147.1
RefSeq Size 3637 bp
RefSeq ORF 1596 bp
Locus ID 71704
UniProt ID Q91X46
Cytogenetics 14 16.09 cM
MW 60.7 kDa
Summary Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027871" in other vectors (4)
SKU Description Size Price
MC217612 Arhgef3 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (Arhgef3), (10ug) 10 ug
$545.00
MG217816 Arhgef3 (tGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (Arhgef3), (10ug) 10 ug
$694.00
MR217816L3 Lenti ORF clone of Arhgef3 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (Arhgef3) 10 ug
$794.00
MR217816L4 Lenti ORF clone of Arhgef3 (mGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (Arhgef3) 10 ug
$794.00

Plasmid Map for MR217816

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217816
Circular map for MR217816

file-alt Citations

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