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Arhgef3 (NM_027871) Mouse Untagged Clone

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SKU
MC217612
Arhgef3 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (Arhgef3), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $545.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Arhgef3
Synonyms 1200004I24Rik; 9830169H03Rik; C76747; Xpln
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217612 representing NM_027871
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGTCGGAGCGCCCCATGGTGTGGTGCTGTTTTTTTCTCCGTGCACAGCGGAAACGAAAACAGAGTT
CCCAGGATGAAGATGCTGTCAGCCTGTGCAGTCTTGACATAAGTGAACCTAGTAACAAAAGGGTCAAACC
CCTTTCCAGAGTCACATCGCTAGCAAACCTCATTCCACCTGTGAAGACCACACCATTAAAGCGCTTCAGC
CAGACCTTGCAGCGGTCCATCAGCTTCCGCAGCGAGAGCCGCCCCGACATCCTTGCCCCCCGAGCTTGGT
CCAGAAATGCCACCTCCTCAAGCACAAAGCGGAGAGACAGCAAACTGTGGAGTGAGACCTTCGATGTGTG
TGTCAATCAGGTGCTCACGGCCAAGGAGATCAAGAGACAGGAGGCGATCTTTGAACTTTCTCAAGGTGAA
GAAGACTTGATAGAGGACTTGAAATTAGCAAAAAAGGCCTACCACGACCCCATGCTGAAACTCTCCATAA
TGACGGAACAGGAACTGAATCAGATTTTTGGGACCCTGGACTCTCTAATTCCTCTCCATGAAGAGCTCCT
CAGTCAGCTTCGAGATGTTCGGAAACCCGATGGCTCGACTGAGCACGTTGGTCCCATCCTCGTGGGCTGG
CTGCCCTGTCTCAGCTCCTACGACAGCTACTGCAGCAATCAAGTCGCCGCCAAAGCCCTGCTGGACCACA
AAAAGCAGGACCACAGAGTGCAGGACTTCCTCCAGCGCTGTTTAGAATCACCCTTCAGTCGCAAACTCGA
TCTCTGGAATTTCCTTGATATTCCCCGGAGCCGGCTAGTGAAGTATCCTCTGCTCCTTCGAGAAATCCTG
AGGCATACACCAAATGATAACCCAGACCAGCAGCACCTGGAAGAAGCTATAAACATCATCCAGGGAATTG
TGGCAGAAATCAACACCAAGACTGGGGAATCGGAATGCCGCTATTACAAAGAGCGGCTCCTTTACCTGGA
AGAAGGCCAGAAAGATTCTCTGATTGACAGCTCAAGGGTCCTGTGTTGTCATGGAGAGCTGAAAAATAAC
CGGGGTGTGAAACTCCATGTTTTCCTGTTCCAAGAAGTGCTGGTGATCACCCGAGCTGTCACTCACAACG
AGCAGCTCTGCTATCAGTTGTACCGACAGCCCATCCCTGTGAAGGACCTCACGCTGGAAGACCTGCAGGA
TGGAGAAGTGCGGCTGGGTGGCTCCCTGCGCGGGGCCTTCAGCAACAACGAGAGAGTTAAAAACTTCTTC
AGAGTCAGTTTCAAAAATGGATCCCAAAGTCAGACCCACTCACTACAAGCCAACGACACCTTCAACAAAC
AGCAGTGGCTCAACTGTATCCGTCAGGCAAAAGAAACTGTTCTGTCTGCTGCCGGCCAGGCTGGCTTGCT
TGACTCTGAGGGACTGGTCCAGGGTCCCGGCACTGAGAACAGGGAGCCACAGGGAGAAACAAAACTTGAG
CAGATGGACCAATCGGACAGCGAGTCGGACTGCAGTATGGACACCAGTGAGGTCAGCCTTGAGTGTGAGC
GGATGGAACAGACAGACGCTTCCTGTGCAAACAGCAGGCCCGAGGAGAGTGTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_027871
Insert Size 1596 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027871.2, NP_082147.1
RefSeq Size 3637 bp
RefSeq ORF 1596 bp
Locus ID 71704
UniProt ID Q91X46
Cytogenetics 14 16.09 cM
Summary Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) has multiple differences at its 5' end, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 2) is shorter and has a distinct N-terminus, compared to isoform 1.
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"NM_027871" in other vectors (4)
SKU Description Size Price
MG217816 Arhgef3 (tGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (Arhgef3), (10ug) 10 ug
$694.00
MR217816 Arhgef3 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (Arhgef3) 10 ug
$289.00 $494.00
MR217816L3 Lenti ORF clone of Arhgef3 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (Arhgef3) 10 ug
$794.00
MR217816L4 Lenti ORF clone of Arhgef3 (mGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 3 (Arhgef3) 10 ug
$794.00

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