Htr5a (NM_008314) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR217656
Htr5a (Myc-DDK-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 5A (Htr5a)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Htr5a
Synonyms Htr5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217656 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCTGCCTGTAAACTTGACCTCCTTTTCTCTCTCTACTCCCTCCTCTTTGGAACCTAACCGCAGCT
TGGACACGGAAGTCCTGCGCCCTAGTCGGCCTTTTCTCTCAGCTTTCCGAGTGCTAGTCCTGACTTTGTT
GGGCTTTCTAGCTGCGGCCACATTCACTTGGAACCTGCTGGTGCTGGCTACCATCCTCAAGGTACGCACC
TTCCACCGAGTACCACACAACCTGGTAGCTTCCATGGCCATCTCGGATGTGCTAGTGGCTGTGCTGGTTA
TGCCACTGAGCCTGGTACATGAGCTGTCTGGGCGCCGCTGGCAGCTGGGCCGGCGTCTATGCCAGCTGTG
GATCGCATGTGACGTGCTCTGCTGTACTGCCAGCATCTGGAATGTGACAGCAATAGCTCTGGACCGCTAC
TGGTCAATCACGCGCCACCTGGAGTACACACTCCGTACCCGCAAGCGTGTCTCCAATGTGATGATCCTGC
TCACCTGGGCACTCTCCACTGTCATCTCTCTGGCTCCACTGCTATTTGGCTGGGGAGAGACTTATTCTGA
GCCCAGTGAGGAATGCCAAGTCAGTCGCGAGCCTTCCTACACCGTGTTCTCCACCGTGGGTGCCTTCTAC
CTGCCGCTGTGCGTGGTGCTCTTTGTGTACTGGAAAATTTACAGGGCGGCGAAATTCCGCATGGGCTCCA
GGAAGACCAACAGCGTCTCCCCCGTACCCGAAGCTGTGGAGGTGAAGAATGCTACGCAACATCCCCAGAT
GGTGTTCACGGTCCGCCATGCCACCGTCACTTTCCAGACAGAAGGGGATACGTGGAGGGAGCAGAAGGAG
CAAAGGGCAGCCCTCATGGTGGGCATCCTCATCGGAGTGTTTGTGCTCTGCTGGTTCCCTTTCTTCGTCA
CAGAGCTCATCAGTCCCCTGTGTTCCTGGGACGTCCCTGCCATCTGGAAGAGCATCTTCCTGTGGTTGGG
CTATTCTAATTCCTTCTTCAACCCACTCATCTACACAGCATTCAACAGGAGCTACAGCAGTGCTTTCAAG
GTCTTCTTCTCCAAGCAACAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR217656 protein sequence
Red=Cloning site Green=Tags(s)

MDLPVNLTSFSLSTPSSLEPNRSLDTEVLRPSRPFLSAFRVLVLTLLGFLAAATFTWNLLVLATILKVRT
FHRVPHNLVASMAISDVLVAVLVMPLSLVHELSGRRWQLGRRLCQLWIACDVLCCTASIWNVTAIALDRY
WSITRHLEYTLRTRKRVSNVMILLTWALSTVISLAPLLFGWGETYSEPSEECQVSREPSYTVFSTVGAFY
LPLCVVLFVYWKIYRAAKFRMGSRKTNSVSPVPEAVEVKNATQHPQMVFTVRHATVTFQTEGDTWREQKE
QRAALMVGILIGVFVLCWFPFFVTELISPLCSWDVPAIWKSIFLWLGYSNSFFNPLIYTAFNRSYSSAFK
VFFSKQQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008314
ORF Size 1071 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008314.1, NM_008314.2, NP_032340.2
RefSeq Size 5576 bp
RefSeq ORF 1074 bp
Locus ID 15563
UniProt ID P30966
Cytogenetics 5 13.24 cM
MW 40.7 kDa
Summary This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008314" in other vectors (4)
SKU Description Size Price
MC208728 Htr5a (untagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 5A (Htr5a), (10ug) 10 ug
$503.00
MG217656 Htr5a (tGFP-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 5A (Htr5a), (10ug) 10 ug
$489.00 $657.00
MR217656L3 Lenti ORF clone of Htr5a (Myc-DDK-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 5A (Htr5a) 10 ug
$757.00
MR217656L4 Lenti ORF clone of Htr5a (mGFP-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 5A (Htr5a) 10 ug
$757.00

Plasmid Map for MR217656

Download sequence for

Cloning scheme for MR217656

Circular map for MR217656

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