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Gjd3 (NM_178596) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217547
Gjd3 (Myc-DDK-tagged) - Mouse gap junction protein, delta 3 (Gjd3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gjd3
Synonyms cx30.2; Gja11; Gjc1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217547 representing NM_178596
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAGTGGGCGTTCCTAGGCTCCCTGCTGGACGCGGTGCAGCTACAGTCGCCGCTCGTGGGTCGTC
TCTGGCTGGTGATCATGCTGATCTTCCGCATCCTGGTGCTGGCCACGGTGGGAGGTGCGGTGTTCGAGGA
CGAGCAGGAGGAGTTCGTGTGTAACACGTTGCAGCCCGGCTGTCGCCAGACCTGCTACGATCGCGCCTTC
CCGGTGTCCCACTACCGCTTCTGGCTCTTCCACATCCTGCTGCTGTCGGCGCCGCCGGTGCTGTTCGTCA
TCTACTCCATGCACCAGGCCAGCAAGGAGGCGGGTGGTGCGCAGCTGGCCCCGCCGTGCGCGCGCGGGCG
TGCCGAGGCGCCGTGCTCCCCGTGCGCCCTGCGCGCTCGCCGCGCGCGCCGCTGCTACCTGCTGAGCGTG
GCTCTGCGCCTGCTCGCCGAGCTGGCTTTCCTGGGCGGCCAGGCGCTGCTCTACGGCTTCCGCGTGGACC
CGCACTACGCGTGCGCCGGGCCACCTTGTCCGCACACGGTCGACTGTTTCGTGAGCCGGCCCACCGAGAA
GACCGTCTTCGTGGTCTTCTACTTCGCCGTCGGGCTGCTGTCGGCGCTGCTCAGCGTGGCGGAGCTGGGT
CACCTGCTCTGGAAGGGTCGCCAGCGCGCCAAGCTGCTCCCGCCGCCGCCGCCGTCGCCCTCTTTGCCAT
CGCAGCGCGGGGACCCCGACCCTTTCGGCCCGCCAGCCTACGCGCACCGCTCACCGGCAGGCGACAGCGA
GGGCGAAGGCGGCAGCGGCCACAGCAAAGCGTCGCTGGCTACCGTGCGCCAGGACCTGGCCATC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217547 representing NM_178596
Red=Cloning site Green=Tags(s)

MGEWAFLGSLLDAVQLQSPLVGRLWLVIMLIFRILVLATVGGAVFEDEQEEFVCNTLQPGCRQTCYDRAF
PVSHYRFWLFHILLLSAPPVLFVIYSMHQASKEAGGAQLAPPCARGRAEAPCSPCALRARRARRCYLLSV
ALRLLAELAFLGGQALLYGFRVDPHYACAGPPCPHTVDCFVSRPTEKTVFVVFYFAVGLLSALLSVAELG
HLLWKGRQRAKLLPPPPPSPSLPSQRGDPDPFGPPAYAHRSPAGDSEGEGGSGHSKASLATVRQDLAI

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_178596
ORF Size 834 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178596.3
RefSeq Size 837 bp
RefSeq ORF 837 bp
Locus ID 353155
UniProt ID Q91YD1
Cytogenetics 11 D
MW 30.7 kDa
Summary One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.UniProtKB/Swiss-Prot Function
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"NM_178596" in other vectors (4)
SKU Description Size Price
MC214642 Gjd3 (untagged) - Mouse gap junction protein, delta 3 (Gjd3), (10ug) 10 ug
$330.00
MG217547 Gjd3 (tGFP-tagged) - Mouse gap junction protein delta 3 (Gjd3), (10ug) 10 ug
$500.00
MR217547L3 Lenti ORF clone of Gjd3 (Myc-DDK-tagged) - Mouse gap junction protein, delta 3 (Gjd3) 10 ug
$600.00
MR217547L4 Lenti ORF clone of Gjd3 (mGFP-tagged) - Mouse gap junction protein, delta 3 (Gjd3) 10 ug
$600.00

Plasmid Map for MR217547

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217547
Circular map for MR217547

file-alt Citations

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