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Sema3d (NM_028882) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217350
Sema3d (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $1,068.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sema3d
Synonyms 4631426B19Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217350 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGTTACTAAAGATGAGAACCCAAGATCCAGAAGTCAAGATCTTCACCTTTTTCATGCTTGGATGA
TGTTAATCATGACGGTGCTCTTTCTTCCTGTCACTGAAACGTCTAAACAAAATATTCCAAGACTCAAGCT
AACCTACAAAGACTTGCTGCTTTCAAACACCTGTATCCCCTTTTTGGGTTCATCAGAAGGATTGGATTTC
CAGACTCTTCTTTTGGATGAGGAGAGGGGCATACTGCTCCTAGGAGCCAAAGACCATGTCTTCCTGCTCA
GTCTGGTTGACTTGAACAAGAATTTTAAGAAGATTTATTGGCCTGCTGCAAAAGAACGAGTGGAGCTATG
TAAATTAGCTGGGAAAGATGCCAATGCAGAATGTGCAAATTTCATCCGTGTGCTTCAACCCTATAATAAG
ACTCACGTTTACGTGTGTGGAACTGGAGCGTTTCATCCGCTGTGTGGGTACATTGATCTCGGCGCCAACA
AGGAGGAACTCATATTTAAACTAGACACGCACAACCTGGAGTCTGGCAGACTGAAATGTCCCTTTGATCC
TCAGCAGCCTTTTGCTTCAGTAATGACAGATGAGCACCTCTACTCTGGAACAGCTTCTGATTTCCTTGGC
AAAGACACTGCATTCACAAGGTCTCTGGGGCTAATGCAGGACCACCATTCCATCAGAACTGACATTTCAG
AGCACCACTGGCTCAATGGAGCAAAATTTATCGGAACATTCCCCATTCCAGACACCTATAATCCAGATGA
TGATAAAATATATTTCTTCTTTCGAGAATCATCCCAGGAAGGCAGTACTTCTGACAGAAGCATTCTTTCA
AGAGTTGGAAGAGTTTGTAAGAATGATGTAGGTGGGCAACGAAGTCTGATAAACAAATGGACAACTTTTC
TAAAGGCAAGACTGATTTGCTCGATTCCTGGAAGCGATGGGGCAGATACCCATTTTGATGAACTCCAAGA
TATTTACTTACTCCCTACGAGAGATGAAAGAAATCCTGTAGTATATGGAGTCTTTACCACAACCAGCTCC
ATCTTCAAAGGCTCTGCTGTCTGTGTGTACAGCATGGCTGATATCCGAGCAGTCTTTAATGGTCCCTATG
CTCATAAGGAAAGTGCTGACCATCGCTGGGTGCAATATGATGGAAGGATACCTTACCCCCGACCTGGAAC
GTGTCCAAGCAAAACCTATGACCCACTGATTAAGTCCACCCGAGACTTTCCAGACGATGTTATCAGTTTC
ATAAGGCGGCACCCTGTGATGTATAAGTCCGTGTACCCAGTGGCAGGAGCACCGACCTTCAAGAGAATCA
ACGTGGATTACAGACTGACGCAGATAGTGGTGGATCACGTGGTCGCTGAAGACGGGCAGTATGATGTCAT
GTTTCTCGGAACAGACATTGGAACAGTCCTGAAAGTTGTGAGCATCTCCAAGGAGAAGTGGAATATGGAA
GAGGTCGTACTGGAGGAGCTTCAGGTATTCAAGCACCCAACAGCTATCTTGAACATGGAGTTGTCGCTGA
AGCAGCAACAGTTGTACGTTGGTTCCTGGGATGGATTGGTCCAGCTCTCCTTGCACAGATGCGACACTTA
CGGGAAAGCATGTGCAGACTGCTGTCTCGCCAGAGACCCTTACTGTGCCTGGGATGGAAATGCTTGCTCC
AGATATGCACCCACGTCTAAAAGGCGAGCTAGACGCCAGGATGTAAAATATGGGGACCCAATCACTCAGT
GCTGGGACATAGAAGACAGCATTAGTCATGAAACAGCTGATGAAAAGGTGATTTTTGGAATTGAATTTAA
TTCAACCTTTTTGGAGTGTATACCTAAATCCCAACAAGCCTCTGTTGAGTGGTACATCCAGCGGTCAGGA
GATGAGCATCGAGAGGAGTTGAAACCTGATGAAAGGATCATCAAAACTGACTATGGGCTACTGATTCGAA
GTCTGCAGAAGAAGGATTCTGGGATGTATTACTGCAAAGCACAGGAGCACACTTTCATCCACACCATAGT
GAAGCTGACTTTGAATGTCATTGAGAATGAACAGATGGAAAATACCCAGAGGGCAGAATACCAGGAGGGG
CAGGTCAAGGATCTGTTGGCTGAGTCACGGTTGAGATACAAAGACTACATCCAAATCCTTAGCAGCCCGA
ACTTCAGCCTGGACCAGTACTGTGAGCAGATGTGGTACAAGGAGAAGCGGAGACAGCGCAACAAGGGCAG
CCCAAAGTGGAAGCACATGCAGGAAATGAAGAAGAAACGAAATCGACGACATCACAGAGACCTCGATGAG
CTCCAGAGATCAGTAGCTACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217350 protein sequence
Red=Cloning site Green=Tags(s)

MNVTKDENPRSRSQDLHLFHAWMMLIMTVLFLPVTETSKQNIPRLKLTYKDLLLSNTCIPFLGSSEGLDF
QTLLLDEERGILLLGAKDHVFLLSLVDLNKNFKKIYWPAAKERVELCKLAGKDANAECANFIRVLQPYNK
THVYVCGTGAFHPLCGYIDLGANKEELIFKLDTHNLESGRLKCPFDPQQPFASVMTDEHLYSGTASDFLG
KDTAFTRSLGLMQDHHSIRTDISEHHWLNGAKFIGTFPIPDTYNPDDDKIYFFFRESSQEGSTSDRSILS
RVGRVCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTHFDELQDIYLLPTRDERNPVVYGVFTTTSS
IFKGSAVCVYSMADIRAVFNGPYAHKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISF
IRRHPVMYKSVYPVAGAPTFKRINVDYRLTQIVVDHVVAEDGQYDVMFLGTDIGTVLKVVSISKEKWNME
EVVLEELQVFKHPTAILNMELSLKQQQLYVGSWDGLVQLSLHRCDTYGKACADCCLARDPYCAWDGNACS
RYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQASVEWYIQRSG
DEHREELKPDERIIKTDYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEYQEG
QVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQMWYKEKRRQRNKGSPKWKHMQEMKKKRNRRHHRDLDE
LQRSVAT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028882
ORF Size 2331 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028882.4, NP_083158.3
RefSeq Size 6521 bp
RefSeq ORF 2334 bp
Locus ID 108151
UniProt ID Q8BH34
Cytogenetics 5 A1
MW 89.5 kDa
Summary Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028882" in other vectors (4)
SKU Description Size Price
MC221516 Sema3d (untagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d), (10ug) 10 ug
$1,068.00
MG217350 Sema3d (tGFP-tagged) - Mouse sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3D (Sema3d), (10ug) 10 ug
$1,268.00
MR217350L3 Lenti ORF clone of Sema3d (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d) 10 ug
$1,368.00
MR217350L4 Lenti ORF clone of Sema3d (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d) 10 ug
$1,368.00

Plasmid Map for MR217350

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217350
Circular map for MR217350

file-alt Citations

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