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Micall1 (NM_177461) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217273
Micall1 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing -like 1 (Micall1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $797.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Micall1
Synonyms AI448246; D15Mit260; D15N2e; EST573322; EST820961; MIRab13; mKIAA1668
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217273 representing NM_177461
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGGCCGCGGGGCGCTCTCCTGGCCTGGTGTCGCCGCCAGTGCGAGGGCTACCGCGGCGTGGACA
TCCGCGACCTGAGCAGCTCCTTCCGGGACGGCCTGGCTTTCTGCGCCATCCTGCACCGGCACCGGCCCGA
CCTGCTCGACTTCCAATCTCTCTCCAAGGAAAATGTCTTCGAGAATAATCGATTGGCCTTTGAAGTGGCT
GAAAAGGAACTGGGCATCCCTGCTCTCTTGGACCCCAATGACATGGTCTCCATGAGTGTCCCTGACTGTC
TCAGCATCATGACCTATGTGTCCCAGTACTACAACCACTTCACCAGTTCTGGCCAAGCTGCTGCCTCACC
ACCCAAACCAGGAAAGGACCCAGCACCCCCCTCCCCCACATCCACATCACCCGCTGTGCAGCCAGGAGAG
GAGGCTCAGGGTGACGACCTCTCCCCAGACAGCCTGTCCGAGCAGGGCAAACAGCAGCCCCCGAGCAGCG
CATGCGCAGCCTGTGGGCAGCGGGTGCACCTGGTGCAGCGGTACTTGGCGGAGGGCAGACTCTACCACCG
GCACTGCTTCCGATGTCGGCAGTGTTCCAGCACGCTGGTCCCAGGCTCTTACAGTAGTGGGCCCGAAGAA
GGCACCTTTGTGTGTGCAGAACGCTGCACCAGGCTGGGTCCGGGAAGTCGGTCAGGAACTAGGCTCCTTT
CACAGCAAAGGCAGCAGCCAGCGGCGGCAGAAGCTAAAGATGCAGAGGATAATGACCCAAGCCTGAGTGT
GGCTGCAGTGGCTGAGGCAGACAGGCTCCAGGCCAGCTCCGAGGTACAGTTCCACACCCCAACCAAGCCA
CCTCTTCCCAGCAAGCCCCAGGAACTGGCCAGTCCCCCTGGTGGCCGGCCCACACCTGCCCCCAGGAAGG
CTTCCGAGAGTTCAGCCTTAACTCCCCCAACGCCAAGGCCTCGGTCTAGCCTGCAGCAAGATGGCACGGT
GGAGCAGAGTGTCAGCAGTGGCCTTGTGAACGGAAGACTACAGGAACCGCCTGTTCCCAAGCCAAGAGGG
ACACCCAAGCTGTCAGAGAGGATGGCAGCGCCAAGGAAGGATCCCCCATGGATCACATTGGTGCAGACAG
AGCCAAAGAAGAAGCCAGCCCCCCAGCCGCCCAGCAGTGGTCCTGGGCCACTGAGTCAGGCCTATAGGCA
GGTGGAGGACGGAGGCCTGGAGGAGCAGACCCAGAAAAGCTCGGGAACTGAGCCAGAGCCCAAGCCCTAC
AACCCCTTTGAGGAAGAAGAGGAAGAAGAAGGAGAGCCAGCCCCACCAGTACCCAGCCCATCTCTAGCCC
CACCAGTACCCAGCCCATCTCCAGCCCCACCAGTACCCAGCCCTGCGCCTGCCCCGTCAGAGGCCACCCC
CAAGTCCCTGCACCCCTGGTACGGCATCACTCCTACCAGCAGCCCCAAGACAAAGAAGCGCCCTGCCCCG
AGAGCACCCAGTGCTTCCCCACTTGCTATCCACGCCTCCCGCCTGTCACACTCAGAACCTCCTTCAGCTA
CGCCATCACCAGCCCTTAGCGTGGAGAGTCTGTCATCCGAGAGCTCCAGTCACACTGCCAATGCAGAGCC
TTTGGAGCCGCCTGCCGTACCCAAGAGCTCCTCGGATCCTGCTGTGCACGTGCCTGGTACCCCTGGCACC
TCTGGGAACTCTGTCACCCCCTCAGCCAACTCTTCCCTGTCGTCTTCTGGGGAGCTAGGGCAGCCTAGTG
GTGAACAAATGCTGCAGGCCAGGACGAAGGGCAGCGCGGGTACCCATTCTACCAAGCCCTTCAGTGGTGC
CACCCCCACCCCCTTCTTGTTGGCTGGAGACCGGAACCCTGCCCCTCCCGTGGGGAGCGCATCCCCACAG
CTGCAAATCAAGTCTTCCTGCAAGGAGAATCCTTTCAACCGGAAACCATCTCCCTCCGCGTCTCCAACTG
TAAGGAAGGCCACCAAAGGAGCCAAGCCTGTGAGGCCACCTGCCCCCGGACACGGCTTTCCACTCATCAA
ACGCAAGGTCCAGGCTGACCAGTACATTCCCGAGGAGGACATCTACGGTGAGATGGACAATATTGAGCGC
CAGCTGGATGCCCTGGAACACAGTGGGGTTCTGTTGGAGGAGAAGCTGCGTGGCGGAGCAAATGAGGGCA
GCGAGGACGACATGCTGGTGGACTGGTTCAAACTCATCCATGAGAAGCACCTACTAGTGCGGCGAGAGTC
GGAGCTCATCTATGTCTTCAAGCAGCAGAACCTGGAGCAGCGCCAGGCTGATGTGGAGTTCGAGCTCCGG
TGCCTTCTCAACAAGCCAGAAAAGGACTGGACAGATGAGGATCGGGCCAGAGAGAAGGTGCTGATGCAGG
AACTCATGACCCTCATCGAGCAGCGAGATGCCATCGTGAACTGCCTGGACGAGGACCGGCAGAGGGAAGA
GGAGGAAGACAAGATGTTGGAAACTATGATCAAGAAGAAAGATTTCCAAAGAGAGGCCGAGTCTGACAGT
AAGAAGAAAGGCAAGTTCAAGACCATAAAGGTGCTGAAGTTTCTGGGGAATAAACGAGAAGCCAAGAGCA
AGGCCCCAGGGGACAAGAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217273 representing NM_177461
Red=Cloning site Green=Tags(s)

MAGPRGALLAWCRRQCEGYRGVDIRDLSSSFRDGLAFCAILHRHRPDLLDFQSLSKENVFENNRLAFEVA
EKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYYNHFTSSGQAAASPPKPGKDPAPPSPTSTSPAVQPGE
EAQGDDLSPDSLSEQGKQQPPSSACAACGQRVHLVQRYLAEGRLYHRHCFRCRQCSSTLVPGSYSSGPEE
GTFVCAERCTRLGPGSRSGTRLLSQQRQQPAAAEAKDAEDNDPSLSVAAVAEADRLQASSEVQFHTPTKP
PLPSKPQELASPPGGRPTPAPRKASESSALTPPTPRPRSSLQQDGTVEQSVSSGLVNGRLQEPPVPKPRG
TPKLSERMAAPRKDPPWITLVQTEPKKKPAPQPPSSGPGPLSQAYRQVEDGGLEEQTQKSSGTEPEPKPY
NPFEEEEEEEGEPAPPVPSPSLAPPVPSPSPAPPVPSPAPAPSEATPKSLHPWYGITPTSSPKTKKRPAP
RAPSASPLAIHASRLSHSEPPSATPSPALSVESLSSESSSHTANAEPLEPPAVPKSSSDPAVHVPGTPGT
SGNSVTPSANSSLSSSGELGQPSGEQMLQARTKGSAGTHSTKPFSGATPTPFLLAGDRNPAPPVGSASPQ
LQIKSSCKENPFNRKPSPSASPTVRKATKGAKPVRPPAPGHGFPLIKRKVQADQYIPEEDIYGEMDNIER
QLDALEHSGVLLEEKLRGGANEGSEDDMLVDWFKLIHEKHLLVRRESELIYVFKQQNLEQRQADVEFELR
CLLNKPEKDWTDEDRAREKVLMQELMTLIEQRDAIVNCLDEDRQREEEEDKMLETMIKKKDFQREAESDS
KKKGKFKTIKVLKFLGNKREAKSKAPGDKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177461
ORF Size 2610 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177461.1, NP_803412.1
RefSeq Size 6708 bp
RefSeq ORF 2613 bp
Locus ID 27008
UniProt ID Q8BGT6
Cytogenetics 15 37.7 cM
MW 94.1 kDa
Summary Probable lipid-binding protein with higher affinity for phosphatidic acid, a lipid enriched in recycling endosome membranes. On endosome membranes, may act as a downstream effector of Rab proteins recruiting cytosolic proteins to regulate membrane tubulation. May be involved in a late step of receptor-mediated endocytosis regulating for instance endocytosed-EGF receptor trafficking. Alternatively, may regulate slow endocytic recycling of endocytosed proteins back to the plasma membrane. May indirectly play a role in neurite outgrowth.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_177461" in other vectors (4)
SKU Description Size Price
MC222320 Micall1 (untagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing -like 1 (Micall1), (10ug) 10 ug
$877.00
MG217273 Micall1 (tGFP-tagged) - Mouse microtubule associated monoxygenase calponin and LIM domain containing -like 1 (Micall1), (10ug) 10 ug
$997.00
MR217273L3 Lenti ORF clone of Micall1 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing -like 1 (Micall1) 10 ug
$1,097.00
MR217273L4 Lenti ORF clone of Micall1 (mGFP-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing -like 1 (Micall1) 10 ug
$1,097.00

Plasmid Map for MR217273

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217273
Circular map for MR217273

file-alt Citations

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