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Krt6b (NM_010669) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217066
Krt6b (Myc-DDK-tagged) - Mouse keratin 6B (Krt6b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $516.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Krt6b
Synonyms BB144589; Krt2-6b; mK6b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217066 representing NM_010669
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTACCAAAACCACCATCAAGAGTCAAACTAGCCACCGTGGCTACAGTGCCAGCTCAGCCAGAGTGC
CTGGGCTCAACCGCTCTGGCTTCAGCAGTGTGTCCGTGTGCCGCTCCCGGGGCAGCGGTGGCTCCAGTGC
AATGTGTGGAGGAGCTGGCTTTGGCAGCAGGAGCCTCTATGGTGTGGGGAGCTCCAAGAGGATCTCCATC
GGAGGGGGCAGCTGTGGCATTGGAGGAGGCTATGGCAGCCGATTTGGAGGAAGCTTCGGCATTGGAGGTG
GAGCTGGTAGTGGCTTTGGCTTCGGTGGTGGAGTTGGCTTTGGTGGTGGCTATGGCGGAGCTGGCTTCCC
GGTGTGCCCCCCTGGAGGCATCCAAGAGGTCACCATCAACCAGAACCTCCTCACCCCTCTGAACGTGCAA
ATCGACCCCACCATCCAGCGGGTCAGGACTGAGGAGAGGGAGCAGATCAAGACCCTCAACAACAAGTTTG
CCTCCTTCATCGACAAGGTCCGGTTCCTGGAGCAGCAGAACAAGGTCCTGGACACCAAGTGGGCCCTGCT
GCAAGAGCAGGGCACCAAGACCGTGAGGCAGAACCTGGAGCCTATGTTTGAGCAGTACATCAGCAACCTC
CGCAGACAGCTGGACAGCATCATTGGAGAGAGGGGTCGCCTGGACTCAGAGCTGAGGAACATGCAGGACA
CAGTGGAGGACTACAAGAGCAAATATGAAGATGAAATCAACAAGCGTACAAAAGCAGAGAATGAATTTGT
AACCGTGAAAAAGGATGTAGATGCTGCCTACATGACCAAGGTTGAACTGCAAGCCAAGGCAGATAGTCTT
GCAGACGAGATCAACTTCCTGAGAGTTATTTATGAGGCAGAACTGTCTCAGATGCAAACTCACATCTCAG
ACACATCTGTGGTCCTCTCCATGGACAACAACCGTAGCCTGGACCTGGACAGCATCATCGCTGAGGTCAA
GGCCCAGTATGAGGACATTGCTCAGAGAAGTCGGGCTGAAGCTGAGTCCTGGTACCAGACTAAATATGAG
GAGCTGCAGGTCACAGCTGGCAGACATGGGGACGACCTGCGCAACACCAAGCAGGAGATTGCTGAGATCA
ACCGCATGATCCAGAGGCTGAGATCTGAGATCGACCACGTTAAGAAGCAGTGTGCCAACCTGCAAGCTGC
TATTGCTGATGCTGAGCAACGTGGGGAGATGGCCCTGAAGGATGCCAGGGGCAAGCTGGAAGGGCTGGAG
GATGCCCTGCAGAAGGCCAAACAGGAAATGGCCAGGCTGCTGAAGGAGTACCAGGAACTCATGAATGTCA
AGCTGGCCCTGGATGTGGAAATTGCCACCTACAGGAAGCTGCTGGAAGGAGAGGAGTGCAGGTTGAATGG
TGAAGGTGTTGGACCAGTCAACATCTCTGTGGTGCAGTCCACCGTGTCCAGCGGCTATGGCAGTGCCGGG
GGTGCCAGCAGCAGCTTAGGCCTGGGTGGAAGCAGCAGCTACTCCTATGGCAGCAGCCATGGCCTTGGAG
GTGGCTTCAGTGCTGGCAGTGGCAGAGCCATCGGAGGTGGCCTCAGCTCTTCTGGTGGCCTCAGCTCTTC
CACCATCAAATACACCACCAGCGCCTCCTCCAGCAGGAAGAGCTACAGGCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217066 representing NM_010669
Red=Cloning site Green=Tags(s)

MSTKTTIKSQTSHRGYSASSARVPGLNRSGFSSVSVCRSRGSGGSSAMCGGAGFGSRSLYGVGSSKRISI
GGGSCGIGGGYGSRFGGSFGIGGGAGSGFGFGGGVGFGGGYGGAGFPVCPPGGIQEVTINQNLLTPLNVQ
IDPTIQRVRTEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKWALLQEQGTKTVRQNLEPMFEQYISNL
RRQLDSIIGERGRLDSELRNMQDTVEDYKSKYEDEINKRTKAENEFVTVKKDVDAAYMTKVELQAKADSL
ADEINFLRVIYEAELSQMQTHISDTSVVLSMDNNRSLDLDSIIAEVKAQYEDIAQRSRAEAESWYQTKYE
ELQVTAGRHGDDLRNTKQEIAEINRMIQRLRSEIDHVKKQCANLQAAIADAEQRGEMALKDARGKLEGLE
DALQKAKQEMARLLKEYQELMNVKLALDVEIATYRKLLEGEECRLNGEGVGPVNISVVQSTVSSGYGSAG
GASSSLGLGGSSSYSYGSSHGLGGGFSAGSGRAIGGGLSSSGGLSSSTIKYTTSASSSRKSYRH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010669
ORF Size 1662 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010669.3
RefSeq Size 2251 bp
RefSeq ORF 1665 bp
Locus ID 16688
Cytogenetics 15 56.97 cM
MW 59.5 kDa
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Documents
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"NM_010669" in other vectors (4)
SKU Description Size Price
MC218949 Krt6b (untagged) - Mouse keratin 6B (Krt6b), (10ug) 10 ug
$568.00
MG217066 Krt6b (tGFP-tagged) - Mouse keratin 6B (Krt6b), (10ug) 10 ug
$716.00
MR217066L3 Lenti ORF clone of Krt6b (Myc-DDK-tagged) - Mouse keratin 6B (Krt6b) 10 ug
$816.00
MR217066L4 Lenti ORF clone of Krt6b (mGFP-tagged) - Mouse keratin 6B (Krt6b) 10 ug
$816.00

Plasmid Map for MR217066

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217066
Circular map for MR217066

file-alt Citations

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