OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Trmt44 (NM_030208) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR216279
Trmt44 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2310079F23 gene (2310079F23Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $653.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Trmt44 or NM_030208"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trmt44
Synonyms 2310079F23Rik; Mettl19
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR216279 representing NM_030208
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGCTGGGTAGAGTGCGGCTAAGCGATCCTGATGCCCTGCTACCTGCAGGCTTCTGGGCCGCCG
TGACCGTGTGGCTGGAGAGGCCACAGGTGGCCAACAAGAGGCTGTGCGGCGCCCGCATGGAAGCCCGCGG
GAGAACTTTGCGGTCCCACGCCCAGGCGGAGTGTGGACCGCGGCAGGGACAGGGACACGGGCTGGAGAGG
GAACCCGGACAGGCTTCTCCTAAAGGAGAGCCAGAGTCGGGACCTAGGGCGAGCCGCGAAGGCACCGCTC
CCGCGGCTGACCTGAACTCGCTATGGGACCGAGTCTCCCAAAGCCTCGTCCACGCCAATCCGGAAATGCT
GGCCTTCCTCTGTGGCCCCTCGTTGGGTCCTCAGCCTGAAGCCGCGCAGGAGCTCGACCTGATTCTCAGG
ACTGTCATCCCGAAAGCGAGCCCGCACTCCCCACTCACAGAGCCGAAGAAAGAACTGGTAGTGCAAGATG
TCTCCAGTGGGAGTGTCACCTTTTTGCCCTTGGAAGAAGATAATGAGGGGAATTTGGAGGTTAAAACGAG
CAACGTGTATCAACTCCATCTCCATCACAACGAAGGAGAATGGTTCATATCTGTTTTAATTTTCTGTCCA
GAGAGATGGCATTCAGATGGTGTTGTCTATCCTAAGCCTGCCTGGCTCGGAGAAGAACTGCTGTCCAAGC
TGGCCAGGTGGGCGGTGGAGAACAGGAAGAGCGAGTTTAAAAGCACACTTTCCCTGGTGTCTATCCTGCG
CTATAGCAGGATGTACCAGGAGCTCAAAGAGAAATACAGAGACATGGTAAAGGTTTGGCCTGAAGTAACA
GATCCTGAAAAGTTTGTCTATGAAGATGTGGCCATTGCTACGTATCTGCTGATCCTATGGGAAGAGGAAA
GGGCTGAGAAGGGAGTCACCACCAAGCAGTCTTTCGTGGACTTGGGGTGTGGAAATGGCCTTCTGGTCCA
CATCTTGAGCAACGAGGGGCACCCAGGTAGAGGGATTGATATCCGAAGAAGAAAAATCTGGGACATGTAT
GGACCCCAAACTCAGCTGGAGGAAGGCTCCATCACTCCGAGTGACGAGACCCTGTTCCCTGGTGTTGATT
GGCTGATTGGTAACCATTCTGATGAGCTCACCCCCTGGATCCCTGTCATTGCAGCCAGGTCTTCCTACAC
CTGTCGTTTCTTTGTCCTGCCCTGCTGTTTTTTCGACTTTGTGGGGCGATACCAGCGGCAGCAGAGCAGG
AAGACACAGTACCGAGAGTATCTGGACTTTGTGCTGGAGGTGGGGCTCTCCTGTGGCTTCCACGTGCAGG
AGGACTGCCTCAGGATTCCTTCTACTAAGCGGGTCTGCCTCATTGGGAAATCCAGAACATACCCGCCTTC
TGCAGAGGTCTGGATGGATGAGCAGAGAACGCGATACCTGCACAGCAGACAGGGCCATCCACAGAGCCGG
CCTGGAGGGGCACATGCACCTTCTGCACCGCAGACTGCTGCTCATGACGCTGGTCTCCAGGACAGTTGCA
GGACCGTCAATGCTGGGTCTGAGTGCGTGCTGGAGGGCCTGGCTGCTGAGCGAGGAGCTGGAGCCCCAGC
CCCAGGACTCTGGGTGCCTGGGTTCTGCCCTAGAGAAAAGGCCGAGCGTGTGAGGAATTGTGCAGCCCTC
CCTCGAGACTTCGTTGACCAAGTGGTTTTGCAGGTGGCAAACTTGTTGTTAGATAGAAAGAAGTTCAACA
CAGGAAACTCTGAAGCCAGGAGTCTGAAGCCCTGGAATGGAGGAGGGAGCCTTTCCTTGGCAGAAGTGGC
TGCAGAACTAAACTCAGAGACGCTGCAGCGGCTGAAGCGGGAGTGTGGAGGCCTACAGACCCTTCTCAAG
AATAGCCATCAAGTGTTTGAAGTTCTGAATGGGCGCGTTCACATCCGTGACTGGCGGCAGGAGCTGCAGA
GGGGAAAGCCTCCAGAAGCCAAGCAGAACCTGTCTGCAGCAGTGTTCAAGACTCGGATCTGCTGGTTCTT
CGCCCACCATCCTGATGGCTGCGTACTGCCTGCTGCCCAATGCCCCTTTGCCCATGGGCCCGAAGAGCTG
CGCCTGTCACAGACCTTGAAGAAGCAGAGGCAGGCTCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR216279 representing NM_030208
Red=Cloning site Green=Tags(s)

MEELGRVRLSDPDALLPAGFWAAVTVWLERPQVANKRLCGARMEARGRTLRSHAQAECGPRQGQGHGLER
EPGQASPKGEPESGPRASREGTAPAADLNSLWDRVSQSLVHANPEMLAFLCGPSLGPQPEAAQELDLILR
TVIPKASPHSPLTEPKKELVVQDVSSGSVTFLPLEEDNEGNLEVKTSNVYQLHLHHNEGEWFISVLIFCP
ERWHSDGVVYPKPAWLGEELLSKLARWAVENRKSEFKSTLSLVSILRYSRMYQELKEKYRDMVKVWPEVT
DPEKFVYEDVAIATYLLILWEEERAEKGVTTKQSFVDLGCGNGLLVHILSNEGHPGRGIDIRRRKIWDMY
GPQTQLEEGSITPSDETLFPGVDWLIGNHSDELTPWIPVIAARSSYTCRFFVLPCCFFDFVGRYQRQQSR
KTQYREYLDFVLEVGLSCGFHVQEDCLRIPSTKRVCLIGKSRTYPPSAEVWMDEQRTRYLHSRQGHPQSR
PGGAHAPSAPQTAAHDAGLQDSCRTVNAGSECVLEGLAAERGAGAPAPGLWVPGFCPREKAERVRNCAAL
PRDFVDQVVLQVANLLLDRKKFNTGNSEARSLKPWNGGGSLSLAEVAAELNSETLQRLKRECGGLQTLLK
NSHQVFEVLNGRVHIRDWRQELQRGKPPEAKQNLSAAVFKTRICWFFAHHPDGCVLPAAQCPFAHGPEEL
RLSQTLKKQRQAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030208
ORF Size 2139 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030208.3, NP_084484.2
RefSeq Size 3784 bp
RefSeq ORF 2142 bp
Locus ID 78890
UniProt ID Q9D2Q2
Cytogenetics 5 B2-B3
MW 79.8 kDa
Summary Probable adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-methyltransferase.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_030208" in other vectors (4)
SKU Description Size Price
MC221024 Trmt44 (untagged) - Mouse RIKEN cDNA 2310079F23 gene (2310079F23Rik), (10ug) 10 ug
$719.00
MG216279 Trmt44 (tGFP-tagged) - Mouse RIKEN cDNA 2310079F23 gene (2310079F23Rik), (10ug) 10 ug
$853.00
MR216279L3 Lenti ORF clone of Trmt44 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2310079F23 gene (2310079F23Rik) 10 ug
$953.00
MR216279L4 Lenti ORF clone of Trmt44 (mGFP-tagged) - Mouse RIKEN cDNA 2310079F23 gene (2310079F23Rik) 10 ug
$953.00

Plasmid Map for MR216279

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR216279
Circular map for MR216279

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products