Sec22c (NM_178677) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR215723
Sec22c (Myc-DDK-tagged) - Mouse SEC22 vesicle trafficking protein homolog C (S. cerevisiae) (Sec22c), transcript variant 1
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sec22c
Synonyms 4932412K21; 5930407I15Rik; C530046H07; Sec22l3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR215723 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCATGATCCTTTTTGCCAGCATCGTACGGGTGAGGGATGGACTGCCCCTCTCAGCCTCCACTGACT
TTTACTACGCCCAGGAGTTTCTGGAATGCAGGAGGCAGCTCAAGACCTTAGCCCAGAGGCTGGCCCGGCA
TCCAGGCCGAGGCTGTGCGGAAAGTTGTGACTTCCTTATCTATTTTTCATCATCGGGGGACGTGGCCTGC
ATGGCCATCTGCTCGCGCCAGTGCCCGGCAGCCATGGCCTTCTGCTTCTTGGAGGCTCTGTGGTGGGACT
TCATAGCTTCCTACGACACCACCTGCGTTGGCCTGGCCTCCCGGCCTTACGCCTTTCTTGAGTTTGACAG
TGTCATTCAGAAAACAAAGTGGCATTTTAACCACATGAGCTCCTCCCAGATGAAGAGTGGCTTAGAAAAG
ATCCAAGAGGAGCTCGAGTTCCAGCCTCCAGCAGTCCTGTCCCTAGAGGACACAGATGTGGCAAATGGCA
TGCTGAACGGCCACACCCCAGTGCACTCTGAGCCTGCTCCGAACCTCCGGATGAAGCCGGTGACAGCCCT
GGGTGTCCTCTCCCTTGTTCTCAACATCATGTGCGCGGCCCTGAACCTCATCCGTGGAGTTCACCTCGCA
GAGCATTCTTTACAGGTTGCCCAAGAGGAAGTTGGAAACATCCTGGCTTTCTTTATTCCTTCTGTGGCCT
GCATCGTCCAGTGTTATCTGTACCTGTTCTACAGTCCAGCCCGGACGCTGAAGGTGCTGCTGATGCTGGC
CTCCATCTGCCTGGGCAATGCGTACCTGCACGGGCTGAGGAACACCTGGCAAATCCTCTTCCACGTAGGA
GTGGCTTTTCTGTCTTCATATCAGATCCTGACCAGACAGCTGCAGGAGAGGCAATCTGACTATGGAGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR215723 protein sequence
Red=Cloning site Green=Tags(s)

MSMILFASIVRVRDGLPLSASTDFYYAQEFLECRRQLKTLAQRLARHPGRGCAESCDFLIYFSSSGDVAC
MAICSRQCPAAMAFCFLEALWWDFIASYDTTCVGLASRPYAFLEFDSVIQKTKWHFNHMSSSQMKSGLEK
IQEELEFQPPAVLSLEDTDVANGMLNGHTPVHSEPAPNLRMKPVTALGVLSLVLNIMCAALNLIRGVHLA
EHSLQVAQEEVGNILAFFIPSVACIVQCYLYLFYSPARTLKVLLMLASICLGNAYLHGLRNTWQILFHVG
VAFLSSYQILTRQLQERQSDYGV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178677
ORF Size 909 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178677.4, NP_848792.2
RefSeq Size 5801 bp
RefSeq ORF 912 bp
Locus ID 215474
UniProt ID Q8BXT9
Cytogenetics 9 F4
MW 33.9 kDa
Summary May be involved in vesicle transport between the ER and the Golgi complex.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178677" in other vectors (5)
SKU Description Size Price
MC204471 Sec22c (untagged) - Mouse SEC22 vesicle trafficking protein homolog C (S. cerevisiae) (Sec22c), transcript variant 1, (10ug) 10 ug
$300.00
MG204236 Sec22c (tGFP-tagged) - Mouse SEC22 vesicle trafficking protein-like C (S 10 ug
$500.00
MG215723 Sec22c (tGFP-tagged) - Mouse SEC22 vesicle trafficking protein homolog C (S. cerevisiae) (Sec22c) transcript variant 1, (10ug) 10 ug
$500.00
MR215723L3 Lenti ORF clone of Sec22c (Myc-DDK-tagged) - Mouse SEC22 vesicle trafficking protein homolog C (S. cerevisiae) (Sec22c), transcript variant 1 10 ug
$600.00
MR215723L4 Lenti ORF clone of Sec22c (mGFP-tagged) - Mouse SEC22 vesicle trafficking protein homolog C (S. cerevisiae) (Sec22c), transcript variant 1 10 ug
$600.00

Plasmid Map for MR215723

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Cloning scheme for MR215723

Circular map for MR215723

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