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Gm136 (NM_001033255) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR214286
Gm136 (Myc-DDK-tagged) - Mouse predicted gene 136 (Gm136)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gm136
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR214286 representing NM_001033255
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCAGAAATCCAAATTACACAGATTTTGTTTGCTGTGCCGTTTGCAATAAAATAATTCCACCAGCCC
CTTTCGGGGAAACCTTCAAACGGATCTATGACTACAAGCCATTTAAGACACGCTTCTATACTCACAAAGA
TATCTTGGATATTGGGGCAAGTATTCTGAACAAAGAAGAAGAGTTTCGAGAGACTGTGTTCAAGGAACAA
ATCAAAAAAGCAGAAGCCAAAGTGTGGGAAAAGGCTGAACTGCTCCAAAAACAAGCCGTGGATCAAGCAG
TTGAAGACGCAGAAGCCAGACACAAATTTGAAATTCGGGTTCTGGAAGAACAACATCAAAAAGACTTAAA
GGCTCTAGAAGACAAAACCAAGGTGAATATGATTCAACAGATGAAGGAAGAGCTGAACAGAGAGCACACA
GCTGCAGAACAGCGTATGGTCCATCGGATCCAGAGGATTATGATGGAGTGTCACCAGGAGAAGATGGAGG
CTGTGAAGAAAGCCAGGGAAGAAGAGAGAAGGATTGCTCAGAAAGCTATCGAAGAAGAGAAAAGCAAGGT
CCTGGAGGAATTTGTAACCACTGGCGTAACCGTCATTAAAGACAAGAAGACAAGCCTGGGCCAGCTGATC
AAAGCAAAGGAGCACGAGATGACCATCTACTACGGCATGGCTCAGAGGCAGAAGCAAGAAGAAGTACAAG
AAGTGCTTCAAGAGGCGGAAAAAACACACCAGGCCACTCTTGACAATATGATGGGCAAACTTGTCAATAC
CCAGGGGGAGCTGCTCTCTGTGGCCAAGCAACTGGGAATCATGACAAACTGGAAAGATTTCCTGGAGGAG
GAACTGCAGGAGACCAGGGCCGCGTTTCAGAAGTATATCAACTACACCTTCCCCAGGCTCTCCCCAGGAC
ACGCCGACTTCATTCTGCCCGAAAGAAAGAAAACACCTTCTATCCTTGCTAAGGAAAATGAGCCGAGGAC
TGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR214286 representing NM_001033255
Red=Cloning site Green=Tags(s)

MIRNPNYTDFVCCAVCNKIIPPAPFGETFKRIYDYKPFKTRFYTHKDILDIGASILNKEEEFRETVFKEQ
IKKAEAKVWEKAELLQKQAVDQAVEDAEARHKFEIRVLEEQHQKDLKALEDKTKVNMIQQMKEELNREHT
AAEQRMVHRIQRIMMECHQEKMEAVKKAREEERRIAQKAIEEEKSKVLEEFVTTGVTVIKDKKTSLGQLI
KAKEHEMTIYYGMAQRQKQEEVQEVLQEAEKTHQATLDNMMGKLVNTQGELLSVAKQLGIMTNWKDFLEE
ELQETRAAFQKYINYTFPRLSPGHADFILPERKKTPSILAKENEPRTD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033255
ORF Size 984 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033255.2, NP_001028427.1
RefSeq Size 1303 bp
RefSeq ORF 987 bp
Locus ID 214568
UniProt ID Q3V037
Cytogenetics 4 A5
MW 38.5 kDa
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Documents
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"NM_001033255" in other vectors (4)
SKU Description Size Price
MC212607 Gm136 (untagged) - Mouse predicted gene 136 (Gm136), (10ug) 10 ug
$330.00
MG214286 Gm136 (tGFP-tagged) - Mouse predicted gene 136 (Gm136), (10ug) 10 ug
$500.00
MR214286L3 Lenti ORF clone of Gm136 (Myc-DDK-tagged) - Mouse predicted gene 136 (Gm136) 10 ug
$600.00
MR214286L4 Lenti ORF clone of Gm136 (mGFP-tagged) - Mouse predicted gene 136 (Gm136) 10 ug
$600.00

Plasmid Map for MR214286

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR214286
Circular map for MR214286

file-alt Citations

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