Smco3 (NM_001039558) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR213783
C030030A07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA C030030A07 gene (C030030A07Rik)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Smco3
Synonyms C030030A07Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR213783 representing NM_001039558
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAGAGTGACTTCCTCTACCCACAAAACCCAAGGAGGCGACAGGAAGTAAACCGCCTTCACCAGC
AGCTGCTGGACTGCTTGTCTGACAGCTTCCAGGTCACCAACAAGCTCACAGGGGTTCTTAATACCCACTT
GGGTTGCAGGCTGGCCTTCATTGAAATGAAAAGCGATGGAACCATCAAAGAAAACTGTGACATCATTATC
CAAGCCATGACAAAAATCCAAAAGGAACTGCAAAAGATTGACGAGGCACTGAAAGACCAACTAGAGCCAA
CCCTTTATAGGAAGCTGCAGGATATTAAGGAAAGGGAGACGGAGAAGATTGCCATCGTGCAGAAGGTCAT
TTCTGTCATCCTGGGAGAAGCTACATCTGCAGCCAGTGCAGTGGCTGTTAAACTGGTGGGCTCAAGTGTC
ACAACTGGCATAATTAGCAAGCTGGTCTCTGTGCTAGCTCACATCGGGACTTCTCTCCTTGGAAGCATTG
GCGTTGCCGTGCTCAGCCTCGGGATAGATATGATCATTCAAGCCATCTTGGGAGCAGTGGAGAGGACACA
GCTTCAGGCAGCCATCAAAAGTTATGAGAAGCACCTGGAGGAGTTCAAGGCTGCCTCAGCAAAGTACCAT
CATGCTATCACTGAGGTTGCCACTGCTGTGAAGCGCCAGCTAAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR213783 representing NM_001039558
Red=Cloning site Green=Tags(s)

MAQSDFLYPQNPRRRQEVNRLHQQLLDCLSDSFQVTNKLTGVLNTHLGCRLAFIEMKSDGTIKENCDIII
QAMTKIQKELQKIDEALKDQLEPTLYRKLQDIKERETEKIAIVQKVISVILGEATSAASAVAVKLVGSSV
TTGIISKLVSVLAHIGTSLLGSIGVAVLSLGIDMIIQAILGAVERTQLQAAIKSYEKHLEEFKAASAKYH
HAITEVATAVKRQLR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039558
ORF Size 675 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039558.2, NP_001034647.2
RefSeq Size 2052 bp
RefSeq ORF 678 bp
Locus ID 654818
UniProt ID Q8BQM7
Cytogenetics 6 G1
MW 25.2 kDa
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Documents
Resources
"NM_001039558" in other vectors (4)
SKU Description Size Price
MC215345 Smco3 (untagged) - Mouse RIKEN cDNA C030030A07 gene (C030030A07Rik), (10ug) 10 ug
$330.00
MG213783 C030030A07Rik (tGFP-tagged) - Mouse RIKEN cDNA C030030A07 gene (C030030A07Rik), (10ug) 10 ug
$500.00
MR213783L3 Lenti ORF clone of C030030A07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA C030030A07 gene (C030030A07Rik) 10 ug
$600.00
MR213783L4 Lenti ORF clone of C030030A07Rik (mGFP-tagged) - Mouse RIKEN cDNA C030030A07 gene (C030030A07Rik) 10 ug
$600.00

Plasmid Map for MR213783

Download sequence for

Cloning scheme for MR213783

Circular map for MR213783

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