Olfr53 (NM_146960) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR213775
Olfr53 (Myc-DDK-tagged) - Mouse olfactory receptor 53 (Olfr53)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfr53
Synonyms IE12; MOR253-5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR213775 representing NM_146960
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTTGTTGAGTCTCAACCAGACAGGAGTGACAGAGTTTGTGCTGGAAGGGTTCTCAGAGCATCCTG
GTCTAAGACTGTTCCTGACAGGCTGCTTCTTGACCCTCTACATGATGGCTCTAATGGGCAACATTGTGAT
CATTGCTTTGGTCACCTCCAGCACTGGACTCCACAATCCCATGTACTTTTTCCTGTGCAACCTGGCTACC
ACGGATATTGTGTGCACCTCCTCTGTGATTCCTAAGGCCCTGATTGGCCTAGTATCTGAGGAAAACATCA
TCACCTTCAAGGGATGTATGGCCCAGCTCTTCTTCCTTGCATGGGCAACATCCGCAGAGCTGTTGCTGCT
CACGGTCATGGCCTATGACCGCTATGTGGCTATCTGCTTTCCCCTACACTACAGCTCTAGGATGAGCCCA
CAGCTCTGTGGAGCACTGGCCGTGGGTGTATGGTCCATCAGTGCTGTGAATGCATCTGTGCACACTGGCC
TGATGACACGGCTGTCATTCTGTGGACCCAAGGTCATCACCCACTTCTTCTGTGAGATACCCCCACTCCT
CCTGCTCTCCTGTAGTTCCACATACATTAATAGTGTTATGACACTTGTGGCAGATGTCTTTCTGGGAGGC
ATCAACTTCATGTTAACCCTGTTATCTTATGGCTTCATCATTGCCAGCATCCTGCGCATGCGTTCTGCTG
AGGGCAAGAGGAAGGCCTTTTCTACCTGCTCATCCCACCTCATCGTGGTCTCAGTGTACTACTCATCTGT
GTTCTGTGCCTATATCAGTCCTGCTTCCAGCTACAGCCCAGAAAGAAGCAAATTTACCTCAGTGCTATAC
TCGGTCGTCAGTCCAACCCTCAACCCCCTCATCTATACACTGAGGAACAAGGATGTCAAGCTTGCCTTGG
GCAGGATGTTGGCCTCTTTCTCACAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR213775 representing NM_146960
Red=Cloning site Green=Tags(s)

MMLLSLNQTGVTEFVLEGFSEHPGLRLFLTGCFLTLYMMALMGNIVIIALVTSSTGLHNPMYFFLCNLAT
TDIVCTSSVIPKALIGLVSEENIITFKGCMAQLFFLAWATSAELLLLTVMAYDRYVAICFPLHYSSRMSP
QLCGALAVGVWSISAVNASVHTGLMTRLSFCGPKVITHFFCEIPPLLLLSCSSTYINSVMTLVADVFLGG
INFMLTLLSYGFIIASILRMRSAEGKRKAFSTCSSHLIVVSVYYSSVFCAYISPASSYSPERSKFTSVLY
SVVSPTLNPLIYTLRNKDVKLALGRMLASFSH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146960
ORF Size 936 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146960.2, NP_667171.1
RefSeq Size 1162 bp
RefSeq ORF 939 bp
Locus ID 258962
Cytogenetics 7 F4
MW 34.2 kDa
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_146960" in other vectors (4)
SKU Description Size Price
MC214088 Olfr53 (untagged) - Mouse olfactory receptor 53 (Olfr53), (10ug) 10 ug
$330.00
MG213775 Olfr53 (tGFP-tagged) - Mouse olfactory receptor 53 (Olfr53), (10ug) 10 ug
$500.00
MR213775L3 Lenti ORF clone of Olfr53 (Myc-DDK-tagged) - Mouse olfactory receptor 53 (Olfr53) 10 ug
$600.00
MR213775L4 Lenti ORF clone of Olfr53 (mGFP-tagged) - Mouse olfactory receptor 53 (Olfr53) 10 ug
$600.00

Plasmid Map for MR213775

Download sequence for

Cloning scheme for MR213775

Circular map for MR213775

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products