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Dnajc21 (NM_030046) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR213586
Dnajc21 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 21 (Dnajc21)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $494.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dnajc21
Synonyms 4930461P20Rik; 9930116P15Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR213586 representing NM_030046
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTGTCACTACGAGGCGCTGGGGGTGCGGCGCGACGCCAGCGAGGAGGAGCTCAAGAAGGCCTATC
GGAAGCTGGCCCTGAGGTGGCACCCGGATAAAAATCTGGACAATGCCGCAGAAGCAGCTGAGCAGTTTAA
ATTAATCCAAGCTGCATATGACGTCTTAAGTGACCCTCAGGAAAGAGCGTGGTACGATAACCACCGAGAG
GCCTTACTTAAAGGAGGGCTCGATGGGGAATATCAAGATGACAGCTTAGATCTGCTTCATTATTTCACGG
TTACCTGTTATTCTGGTTATGGGGACGATGAAAGGGGGTTCTATGCAGTGTATCGTGTTGTCTTTGAGCT
GATTGCAAAAGAAGAACTGGAGTGTATGTCAGAGGGAGATGTTGAGGATTTTCCAACCTTCGGCGACTCC
CAGAGCGACTATGACACGGTGGTCCACCCTTTCTACGCTCACTGGCAGAGTTTCTGCACACAAAAGAATT
TTTCCTGGAAGGAAGAGTATGATACCCGGCAAGCCTCGAACCGCTGGGAAAAACGAGCCATGGAAAAAGA
AAACAAAAAAATTCGAGACAGAGCAAGGAAAGAGAAAAATGAACTGGTGCGCCAGCTGGTAGCCTTCATT
CGTAAACGAGATAAGAGAGTGCAGGCCCATCGGAAGCTTGTGGAGGAGCAGAACGCAGAGAAGGCGAGGA
AGGCAGAGGAGATGAGGAGGCAGCAGAAGCTGAAGCAAGCCAAGCTGGCAGAGCAGTACAGAGAGCAGAG
CTGGATGACCATGGCCAATTTGGAGAAGGAGCTCCAGGAGATGGAAGCAAGGTACGAGAAGGAGTTTGGA
GATGGATCAGATGAAAACGAAGTGGAGGATCAGGAGCCCAGAAACGGATTGGATGGCAAGGACAGCGAGG
AGGCTGAGGAGGCTGAGCTTTACCAGGACCTTTACTGCCCAGCTTGTGACAAATCCTTCAAGACAGAAAA
GGCCATGAAGAATCACGAGAAGTCAAAGAAACATCGGGAAATGGTTGCCTTGTTAAAACAGCAGTTGGAG
GAGGAGGAAGAACAGTTTTCAGGAGTTCAAATGGATGAAAATGTATTGAATGCCAATTCTGAGGAGGAAA
TGGAAGATACACCAAAGCAGAAGCTTTCTAAAAAACAGAAGAAAAAGAAACAGAAATCAGCACAGAATTT
TGATGACAATTTCAATGAAAATGGAACTGAAGAAGGAGGAAAGATTGCTCCAGAAAAGACTAAGTCAAAT
GAAGACAATGCCAAAGAATTAGAAAATAGGCCCCAAGAAAATACCTGTATTACAGAGACCACAGAAGCCT
GTGAGGATCCAAAAAGCGAAGCTAAAAGTGTTCCTAAATCCAAAGGAAAGAAAACCAAAGATGTGAAAAA
ATCTGTCAAAGCACCTGCTGAAGCACAGCCAGTGAGTGATGTCCTCATCAGCTGTGCGACTTGCCACAGT
GAATTTCCATCCCGGAATAAACTGTTTGATCATCTAAAGGCTACTGGTCATGCAAGAGCACCTTCAGCCA
CAGCATCTTTAAACAGTGTAACTAGGAACAAGAAAGAGAAGCGTAGAAGTAGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR213586 representing NM_030046
Red=Cloning site Green=Tags(s)

MKCHYEALGVRRDASEEELKKAYRKLALRWHPDKNLDNAAEAAEQFKLIQAAYDVLSDPQERAWYDNHRE
ALLKGGLDGEYQDDSLDLLHYFTVTCYSGYGDDERGFYAVYRVVFELIAKEELECMSEGDVEDFPTFGDS
QSDYDTVVHPFYAHWQSFCTQKNFSWKEEYDTRQASNRWEKRAMEKENKKIRDRARKEKNELVRQLVAFI
RKRDKRVQAHRKLVEEQNAEKARKAEEMRRQQKLKQAKLAEQYREQSWMTMANLEKELQEMEARYEKEFG
DGSDENEVEDQEPRNGLDGKDSEEAEEAELYQDLYCPACDKSFKTEKAMKNHEKSKKHREMVALLKQQLE
EEEEQFSGVQMDENVLNANSEEEMEDTPKQKLSKKQKKKKQKSAQNFDDNFNENGTEEGGKIAPEKTKSN
EDNAKELENRPQENTCITETTEACEDPKSEAKSVPKSKGKKTKDVKKSVKAPAEAQPVSDVLISCATCHS
EFPSRNKLFDHLKATGHARAPSATASLNSVTRNKKEKRRSR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030046
ORF Size 1593 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030046.2, NP_084322.2
RefSeq Size 2053 bp
RefSeq ORF 1596 bp
Locus ID 78244
UniProt ID E9Q8D0
Cytogenetics 15 A1
MW 61.7 kDa
Summary May act as a co-chaperone for HSP70. May play a role in ribosomal RNA (rRNA) biogenesis, possibly in the maturation of the 60S subunit. Binds the precursor 45S rRNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_030046" in other vectors (4)
SKU Description Size Price
MC217622 Dnajc21 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 21 (Dnajc21), (10ug) 10 ug
$545.00
MG213586 Dnajc21 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog subfamily C member 21 (Dnajc21), (10ug) 10 ug
$694.00
MR213586L3 Lenti ORF clone of Dnajc21 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 21 (Dnajc21) 10 ug
$794.00
MR213586L4 Lenti ORF clone of Dnajc21 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 21 (Dnajc21) 10 ug
$794.00

Plasmid Map for MR213586

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR213586
Circular map for MR213586

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